Evaluation of in vitro anticancer potential in Punica granatum, Psidium guajava, and Vitis vinifera seed extracts

Abstract

The anticancer potentials of the seed extracts of edible fruits pomegranate (Punica granatum L.), guava (Psidium guajava L.) and grapes (Vitis vinifera L.) were evaluated. Doxorubicin was used as a reference drug. MTT assay was used to determine the anticancer potential of the selected seed extracts. Among the selected edible fruit seeds and their different organic solvent extracts, the ethyl acetate extract of Punica granatum possessed a higher inhibitory effect against lung cancer cell line, compared to other solvent extracts of Psidium guajava and Vitis vinifera fruit seeds, and the IC₅₀ value was 51.25± 1.25 μg/ml, 60.21 ± 1.35 μg/ml and 61.21±1.45μg/ml, respectively. The IC₅₀ of doxorubicin was 49.25 ± 1.85 μg/ml. The inhibitory effects of fruit seeds against lung cancer cell line (A549) could be ranked as P. granatum > P. guajava. >V. Vinifera. In the overall observations of the study, the P. granatum seed extract showed the highest inhibitory effect on lung cancer cell line among the other seeds.