In vitro anti-microbial activity of combined isolates from ethyl acetate extract of Cynodon dactylon and Piper betle

Abstract

The antibacterial effect of isolated compound from Cynodon dactylon and Piper betle was evaluated on both Gram-positive and Gram-negativie bacteria. The in-vitro antibacterial activity was performed by Cylinder or cup plate method. The dried materials were coarsely powdered using an electric blender. Powdered materials (500g) were then packed in soxhlet apparatus and successively extracted with petroleum ether, Benzene, chloroform, ethyl acetate, methanol and water. Each time before extraction with the next solvent, the powdered materials were dried in hot air oven at below 50ºC. About 2 gms of the concentrated ethyl acetate extract was mixed with suitable quantity of silica gel (100-200 mesh). The elute was concentrated by evaporating the solvent and the residues named as CD isolate (90% of Chloroform: 10 % of Ethyl acetate) and PB isolate (50% of Toluene: 50 % of Ethyl acetate) were identified by High performance Thin Layer Chromatography. The selected isolates from Cynodon dactylon and Piper betle and the combination of both were found to have anti-microbial activity in the present study. The MIC value of CD Isolate against gram positive bacteria was 4.4 ± 0.5 mg/ml and against gram negative bacteria was 4.5 mg/ml. The MIC value of PB Isolate against gram positive bacteria was 4.2 mg/ml and against gram negative bacteria was 4.1 mg/ml. The MIC values of Test Antibiotics were found to be 0.001- 0.412 mg/ml and 0.008 -0.412 mg/ml against gram positive bacteria and gram negative bacteria respectively. The result data revealed that the isolates of individual and combined samples were showed better results for both Gram positive and Gram negative organisms. In that it was very effective against S.Typhi when compare to other organisms which are compared with standard solution. The results obtained in the present study suggest that the isolates can be used in treating diseases caused by the test organisms.