Antioxidant and antiproloferative activity of methanolic and ethanolic extract of Excoecarica Agallocha leaves on human breast cancer cell lines MCF-7

Abstract

A comparative free radical scavenging and antiproliferative potential of methanolic and ethanolic extracts of leaves of Excoecaria agallocha (MEEA, EEEA) were investigated. The free radicals such as superoxide, nitric oxide and hydrogen peroxide scavenging activity were evaluated using standard protocols. Tryptan blue assay was performed to evaluate the antiproliferative activity. Quantitative estimation phenols and flavonoids were also done. The EEEA extract exhibited significant antiproliferative and antioxidant activity than the MEEA extract which was comparable to the positive drug doxorubicin and ascorbic acid used in the present study which may be due to the presence of phenols and flavanoids. The antioxidant and anticancer activity observed in the present study may be due to the presence of phytochemicals along with the phenols and flavanoids.