Solution state stress degradation studies of antifilarial drug albendazole

Abstract

This study was conducted to explore the stress degradation studies of antifilarial drug albendazole (ALB) in the solution state. The stress conditions targeted were hydrolytic (acidic, neutral, and basic) as well as oxidative. The drug was degraded under different concentrations of stressor and the time of exposure. All the degraded samples were then subjected to reversed-phase high-performance liquid chromatography using photodiode array (HPLC-PDA) as a detector. The final concentration of stressor, as well as the duration of exposure for each condition, were optimized from the results. The method optimization was done on a C18 stationary phase with a varying mobile phase composition. The parameters of the mobile phase varied were organic modifier, buffer (type and concentration) and gradient as well as run time. The optimized method included acetonitrile as an organic modifier, 10 mM potassium dihydrogen orthophosphate (pH 3.0) as a buffer that was pumped in a gradient mode, and run time was fixed at 57.00 min. The samples were further characterized by subjecting to instrumental technique Liquid Chromatography High-Resolution Mass Spectrometry (LC-HRMS). The results highlighted the degradation products formed under the study conditions. Some of the degradation products were common to all hydrolytic conditions, but some were seen only under oxidative conditions.