Effect of some beta-lactum antibiotics on liver enzyme activities and their residues in vital organs following long term administration in goats

Abstract

Liver biochemical parameters and residue levels were studied in black Bengal goats following daily intramuscular administration of amoxicillin and ceftriaxone at two dose levels (10 and 5mg/kg for amoxicillin and 50 and 25mg/kg for ceftriaxone) for 60 days. Liver aspartate and alanine transminase (ALT and AST) activities were significantly (P<0.05) depressed at both dose levels of amoxicillin and ceftriaxone suggesting liver injury. Catalase, lipid peroxidation, superoxide dismutase and reduced glutathione levels were non-significantly altered by both antibiotics at both dose levels. Both amoxicillin and ceftriaxone caused significant induction of cytochrome P₄₅₀ content of liver tissue of goats at both dose levels. Higher concentration of amoxicillin remained in different tissues of goats on day 60 compared to ceftriaxone. Residues of amoxicillin and ceftriaxone were higher in kidney followed by other organs on day 60 at both dose levels. On day 81 (21 days of withdrawal period) concentration of amoxicillin was below detection limit in different organs at both dose levels. Ceftriaxone could not be detected in most of the organs at both dose levels on day 60 and 81. On histopathological examination, remarkable changes were recorded in liver, kidney and spleen by amoxicillin and mild alterations were noticed in heart and liver by ceftriaxone.