Comprehensive GC-MS and FT-IR Profiling of Coleus zeylanicus essential oil and investigation of its anti-fungal and anti - oxidant activities
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Essential Oil, Hydro-Distillation, GC-MS, Anti-Fungal Activity, Inhibitory Zone, Antioxidant, IC 50
Abstract
To evaluate the leaf volatile constituents of essential oil of Coleus zeylanicus and evaluate their anti-oxidant and anti-fungal activity. The Chemical composition of Coleus zeylanicus essential oil was determined using GC-MS and FT-IR analytical techniques. The antioxidant activity was evaluated using DPPH scavenging assay. The anti-fungal effect was tested against two potential pathogenic fungal strains Candida albicans and Malassezia furfur using agar well diffusion method. The essential oil was profiled by the presence of sesquiterpene hydrocarbons 90.67% of their total composition followed by oxygenated monoterpenes and monoterpene hydrocarbons as 5.3% and 2.1% respectively. The GC-MS results showed 14 compounds from Coleus zeylanicus leaf EO representing 98.07% of the total oil composition. The major component was identified as a-Gurjunene (35.94%), a-bisabolol (10.82%) and G-selinene (4.26%). EO showed remarkable antioxidant activity values of IC50 = 59.78 ± 3.21µg/ml by DPPH scavenging assay. The essential oil showed interesting anti-fungal effects against two pathogenic fungal strains. The most sensible strains to Coleus zeylanicus EO was Malassezia furfur (32.00 ± 0.50mm) compared to that of Candida albicans (15.00 ± 1.25mm). Hence, Coleus zeylanicus EO has potential application against fungal infection and oxidative stress related diseases. However, further investigations are necessary to isolate and investigate the action mechanism of these bioactive compounds.
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