Determination of Balofloxacin in Pharmaceutical formulation by Zero, First and Second order Derivative Spectrophotometric methods

Abstract

In this study, zero, first and second order derivative spectrophotometric methods were developed for quantitative determination of Balofloxacin in pharmaceutical preparation. In zero order spectrophotometry, absorbance values were measured at 286 nm. In first derivative spectrophotometry, amplitude values were measured at 295 nm. In second derivative spectrophotometry, amplitude values were measured at 266 nm. Parameters such as linearity, precision, accuracy were studied according to the International Conference on Harmonization Guidelines. Calibration curves were linear between the concentration range of 10-50 μg/ml, 2-10 μg/ml and 10-50 μg/ml for zero, first and second order derivative spectrophotometric methods. The RSD values of system and method precision for Balofloxacin were less than 2%, the mean recovery value of Balofloxacin was in between 98-102% for pharmaceutical preparation. All the methods developed were successfully applied to a tablet formulation and the results were found to be within the limits.