Ulipristal acetate determination using MBTH

Abstract

A simple visible spectrophotometric method is proposed for the determination of ulipristal acetate present in bulk and tablet formulation. The currently proposed method is established based on MBTH oxidation by ferric ions to form an active coupling species (electrophile), followed by its coupling with the ulipristal in acidic medium to form high intensified green colored chromophore having λmax at 609 nm. Validated the method as per the current guidelines of ICH. Beer’s law was obeyed in the concentration range of 6.25 – 37.50 μg mL-1 with a high regression coefficient (r > 0.999). Reproducibility, accuracy, and precision of the method are evident from the low values of R.S.D. This method can be used in quality control laboratories for routine analysis of ulipristal acetate in bulk drug and pharmaceutical dosage forms.