Chromatographic finger print analysis of budmunchiamines in Albizia amara by HPTLC technique

Abstract

Recently, there has been an increase in the use of herbal drugs for healthcare. Herbal Medicine is a time-tested and valuable resource for healing. In India, the herbal drug market is about $ one billion and the export of plant based crude drugs is around $ 80 million. So it is absolutely essential to provide scientifically proven and evidence based herbal formulations for global acceptance. Chromatographic fingerprint symbolize the active chemical constituents of herbal medicines for desired therapeutic action. This study presents a simple, rapid and selective HPTLC method for separation and determination of Albizia amara. Albizia amara (Fabaceae) is the medicinal plant which has been used to treat piles, diarrhoea, gonorrhoea, leprosy, and leucoderma. The macro cyclic alkaloid Budmunchiamines is the main constituent which has been separated and identified by camag HPTLC. The coarsely dried powdered leaves were extracted with Petroleum Ether (60-80)⁰C by cold maceration, and hot percolation with 90% methanol for 72 h. TLC profile and HPTLC fingerprints were recorded by using silica gel GF₂₅₄ as stationary phase. Separation was performed on pre-coated 10x10cm HPTLC Aluminium sheets. Sample volumes of (5μl) were applied to the 6 tracks contain bandwidth of 5mm and 10 secs/μl using a TLC applicator. The plates were developed using a mobile phase of Chloroform: diethylamine (86:22 v/v). The peak 2, 6 reveals that the presence of macro cyclic alkaloids Budmunchiamines L4, L5 were characterised and confirmed by FTIR, ¹H NMR, MASS and CHN analysis. The results were compared with earlier reports. The developed HPTLC fingerprints will help the manufacturer for quality control and standardization of herbal formulations.