In-Vitro Alterations in Biohostability of three commonly used surgical sutures at pH of 5,6,7

Abstract

Periodontitis is a multifactorial disease, one of the responsible factors include Bacteria present in the oral cavity. Bacteria, like all other organisms, are dependent on the environment for their survival and are influenced by the various host as well as environmental factors for their sustenance. Some of the major environmental factors include nutrition, the presence of oxygen, along with pH, which dictate the nature of the bacteria present as certain bacteria thrive better in acidic environments while others in Alkali environments. In medicine, sutures are extensively used with the aim of post-surgical wound edge approximation or wound healing by the primary union. However, pH is one factor that is constantly changing in the oral cavity due to dietary intake or systemic factors or local accumulation of pus. The present study wished to assess the biohostability of different suture material such as silk, vicryl and chromic gut when pH was changed to 5,6,7 keeping all other environmental factors a constant, including five pieces of sterilised standardised suture lengths & diameter, incubated in ph of 5,6, and 7 exposed to a microbial load of 0.5 McFarland units Lactobacillus subculture in brain heart infusion and incubated at 35 degrees Celsius for four hours to assess the number of bacterial colonies using visual click method. It was found that pH plays a vital role in the Biohostability of suture material as the current study suggests an acidic pH of 5 had more colony-forming units seen among all three suture materials as compared to the other two groups.