Hepatoprotective effect on methanolic extracts of Tagetes erecta leaves and Tridax procumbens against drug induced hepatic injury
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Tagetes erecta, Tridax procumbens, Isoniazid, Rifampicin, Hepatotoxicity, Oxidative stress, Antioxidants
Abstract
The current study was undertaken to evaluate the preclinical efficacy of methanolic extract of Tagetes erecta (METE) and methanolic extract of Tridax procumbens (METP) against Isoniazid and Rifampicin (INH-RIF) induced hepatic injury. Animals were randomly divided into seven groups, vehicle (control) or INH-RIF (50 mg/kg, i.p.), METE (200 and 400mg/kg, p.o.), METP (200 and 400mg/kg, p.o.) and standard silymarin for 28 days. INH-RIF intoxicated rats displayed significant (p<0.05) elevation in serum hepatic markers, lipid peroxidation and decrease in antioxidants like SOD, CAT, Gpx and GSH in liver tissue. Treatment with METE & METP (200 and 400 mg/kg, p.o.) restored the altered biochemical level to normalcy. Thus, the outcome of the study reveals that METE & METP showed promising hepatoprotective activity in INH-RIF induced hepatic damage mediated by its membrane stabilizing and antioxidant effect. All the methanolic plant extracts produce significant hepatoprotective activity against drug induced hepatic injury.
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Abbara, A., Chitty, S., Roe, J. K., Ghani, R., Collin, S. M., Ritchie, A., Kon, O. M., Dzvova, J., Davidson, H., Edwards, T. E., Hateley, C., Routledge, M., Buckley, J., Davidson, R. N., John, L. 2017. Drug- induced liver injury from antituberculous treatment: a retrospective study from a large TB centre in the UK. BMC Infectious Diseases, 17(1):231–231. ISSN 1471-2334.
Ali, M., Ravinder, E., Ramachandram, R. 2001. A new flavonoid from the aerial parts of Tridax procumbens. Fitoterapia, 72(3):313–315. ISSN 0367- 326X.
Baniasadi, S., Eftekhari, P., Tabarsi, P., Fahimi, F., Raoufy, M. R., Masjedi, M. R., Velayati, A. A. 2010. Protective effect of N-acetylcysteine on antituberculosis drug-induced hepatotoxicity. European Journal of Gastroenterology & Hepatology, 22(10):1235–1238. ISSN 0954-691X.
Bhagat, M. S., Kondawar 2019. A comprehensive review on phytochemistry and pharmacological use of Tridax procumbens Linn. Journal of Pharmacognosy and Phytochemistry, 8(4):2349–8234.
Darvin, S. S., Esakkimuthu, S., Toppo, E., Balakrishna, K., Paulraj, M. G., Pandikumar, P., Ignacimuthu, S., Al-Dhabi, N. A. 2018. Hepatoprotective effect of lawsone on rifampicin isoniazid induced hepatotoxicity in in vitro and in vivo models. Environmental Toxicology and Pharmacology, 61:87–94. ISSN 1382-6689.
Gopi, E. G., Jayasri, P. 2012. A concise review on Tegetes eracta. International Journal of Phytopharmacy research, 3(1):16–19.
Huang, Y.S., Chern, H.D., Su,W.J. 2003. Cytochrome P450 2E1 genotype and the susceptibility to antituberculosis drug-induced hepatitis. Hepatology, 37(4):924–930.
Jachak, S.M., Gautam, R., Selvam, C., Madhan, H., Srivastava, A., Khan, T. 2011. Anti-inflammatory, cyclooxygenase inhibitory and antioxidant activities of standardized extracts of Tridax procumbens L. Fitoterapia, 82(2):173–177. ISSN 0367- 326X.
Jadhav, V.B., Thakare, V.N., Suralkar, A.A., Deshpande, A.D., Naik, S.R. 2010. Hepatoprotective activity of Luffa acutangula against CCl4 and Rifampicin induced liver toxicity in rats: a biochemical and histopathological evaluation. Indian Journal of Experimental Biology, 48(8):822–829.
Khulbe, A. 2015. A review on Tagetes erecta. World journal of Pharmaceutical Sciences, 3(3):645–649.
Kumar, A., Khulbe, P. 2016. Influence of abiotic factors and hosts on seasonal dynamic of green lacewing, Chrysoperla carnea (Stephens). Journal of Agri Search, 3(3):645–649. ISSN 2348-8808, 2348-8867.
Marasani, A. 2014. Hepatoprotective activity of Bauhinia variegata against Isonized and Rifampicin induced toxicity in experimental rats. International Journal of Pharmacy and Pharmaceutical Sciences, 6(4):177–181.
Mitchell, J.R., Thorgeirsson, U.P., Black, M., Timbrell, J.A., Snodgrass, W.R., Potter, W.Z., Jollow, D.J., Keiser, H.R. 1975. Increased incidence of isoniazid hepatitis in rapid acetylators: possible relation to hydrazine metabolites. Clinical Pharmacology & Therapeutics, 18(1):70–79. ISSN 0009-9236.
Nannelli, A., Chirulli, V., Longo, V., Gervasi, P. G. 2008. Expression and induction by rifampicin of CAR and PXR-regulated CYP2B and CYP3A in liver, kidney and airways of pig. Toxicology, 252(1-3):105– 112. ISSN 0300-483X.
Osman, A.M., Al-Harbi, A.S. 1993. Effect of desferrioxamine on doxorubicin-induced cardiotoxicity and haematotoxicity in mice. Med Sci Res, 21(5):269–8951.
Pandey, A., Tripathi, S. 2014. A Review on Pharmacognosy, Pre-phytochemistry and Pharmacological analysis of Tridax procumbens. Pharma Tutor, 2(4):78–86. ISSN 2347–7881.
Priyanka, D., Shalini, T., Navneet, V. 2013. A brief study on marigold (tagetes species), a review. International research journal of pharmacy, 4:43– 48.
Rajaram, S.S., Ashvin, G. 2013. Preliminary Phyto- chemical Analysis of Leaves of Tridax Procumbens Linn. International Journal of Science, 2(3):388– 394.
Ramaiah, S. K. 2007. A toxicologist guide to the diagnostic interpretation of hepatic biochemical parameters. Food and Chemical Toxicology, 45(9):1551–1557. ISSN 0278-6915.
Ramappa, V., Aithal, G.P. 2013. Hepatotoxicity Related to Anti-tuberculosis Drugs: Mechanisms and Management. Journal of Clinical and Experimental Hepatology, 3(1):37–49. ISSN 0973-6883.
Sanghavi, N., Srivastava, R., Malode, Y. 2014. Isolation and identification of the flavonoid “Quercetin” from Tridax procumbens linn. International Journal of Pharmaceutical Sciences and Research, 5(4):1454–59.
Sarich, T.C., Adams, S.P., Wright, J.M. 1998. The Role of L-Thyroxine and Hepatic Reductase Activity in Isoniazid-Induced Hepatotoxicity in Rabbits. Pharmacological Research, 38(3):199–207.
Satish, A., Bhalerao, T.S., Kelkar 2012. Phytochemical and pharmacological potential of Tridax Procumbens linn. International Journal of Advanced Biological Research, 2(3):392–395.
Shanmugapriya, A., Maneemegalai, S. 2018. Cardioprotective Potential of Tridax Procumbens against Isoproterenol Induced Myocardial Infarction in Experimental Rats. World Journal of Pharmaceutical Research, 7(10):885–893.
Singh, D., Cho, W.C., Upadhyay, G. 2016. Drug Induced Liver Toxicity and Prevention by Herbal Antioxidants: An Overview. Frontiers in Physiology, 6(363). ISSN 1664-042X.
Sotgiu, G., Sulis, G., Matteelli, A. 2017. Tuberculosis—a World Health Organization Perspective. Microbiology Spectrum, 5(1):7–0036. ISSN 2165-0497.
Tostmann, A., Boeree, M.J., Peters, W. H., Roelofs, H.M., Aarnoutse, R.E., van der Ven, A.J., Dekhuijzen, P.R. 2008. Isoniazid and its toxic metabolite hydrazine induce in vitro pyrazinamide toxicity. International Journal of Antimicrobial Agents, 31(6):577–580. ISSN 0924-8579.
Zhang,S., Liu, X., Bawa-Khalfe, T., Lu, L.-S., Lyu, Y. L., Liu, L. F., Yeh, E. T. H. 2012. Identification of the molecular basis of doxorubicin-induced cardiotoxicity. Nature Medicine, 18(11):1639–1642. ISSN 1078-8956, 1546-170X.
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