Extraction of polyphenols using afroyal gala and granny smith for antimicrobial activity

Abstract

Apples contain many useful human health-related contents. Polyphenols exhibit antimicrobial effects against human pathogens. In this study, the polyphenolic contents are removed by the skin of Royal Gala and Granny Smith, which are the two varieties of apples. The phenolic compounds were removed from fresh fruit peels of Royal Gala and Granny Smith with the solvents: A-(water), B- (ethanol: water). The antimicrobial happenings of the excerpts are verified in contradiction of Escherichia coli and Staphylococcus aureus bacteria by jelly healthy dispersion technique. The extracts from both apples by solvent A presented advanced anti micro bisection as associated with excerpts by solvent B. Among the 2 varieties of apples, the Granny Smith extracts presented a superior antimicrobial result as compared to Royal Gala extracts against the selected microorganisms E-coli and S -aureus. The zone of inhibition by the apple peel extracts is more in case of S -aureus compared with E-coli; this reveals that the organism S-aureus is more sensitive to polyphenolic compounds than Escherichia coli. With this study, it was concluded that the water extract of Granny Smith peels showed superior antimicrobial movement because of advanced amounts of polyphenolic mixtures as associated to Royal Gala and the best solvent to extract polyphenolic compounds from apple peels is water.