Albumin, as a Therapeutic Protein: Potential Source, Application, Isolation and Purification

Ukun MS Soedjanaatmadja; Nadiya Adnani; Wildan Abiyyi; Saadah Diana Rachman; Shabarni Gaffar; Safri Ishmayana; Ari Hardiyanto

Ukun MS Soedjanaatmadja ⁽¹⁾ , Nadiya Adnani ⁽²⁾ , Wildan Abiyyi ⁽³⁾ , Saadah Diana Rachman ⁽⁴⁾ , Shabarni Gaffar ⁽⁵⁾ , Safri Ishmayana ⁽⁶⁾ , Ari Hardiyanto ⁽⁷⁾

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences Universitas, Padjadjaran, Indonesia, Indonesia ,

(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences Universitas, Padjadjaran, Indonesia, Indonesia ,

(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences Universitas, Padjadjaran, Indonesia, Indonesia ,

(4) Department of Chemistry, Faculty of Mathematics and Natural Sciences Universitas, Padjadjaran, Indonesia, Indonesia ,

(5) Department of Chemistry, Faculty of Mathematics and Natural Sciences Universitas, Padjadjaran, Indonesia, Indonesia ,

(6) Department of Chemistry, Faculty of Mathematics and Natural Sciences Universitas, Padjadjaran, Indonesia, Indonesia ,

(7) Department of Chemistry, Faculty of Mathematics and Natural Sciences Universitas, Padjadjaran, Indonesia, Indonesia

Issue
Vol. 12 No. 3 (2021): Volume 12 Issue 3

Keywords:

Albumin, Hypoalbuminemia, HSA, C. gariepinus, P. hypophthalmus

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Abstract

Albumin is one of the plasma proteins found in the human body, which is about 55-60%, and the total normal serum protein level is 3.8-5.0 g/dL. Albumin consists of a single chain of a polypeptide with a molecular weight of 66.5kDa and consists of 585 amino acids. In the albumin molecule, 17 disulfide bonds connect amino acids containing sulfur. The molecular albumin is elliptical so that with such molecular forms, it will not increase the plasma viscosity and dissolve perfectly. Albumin is protein and its action as a transport agent and maintains colloid osmotic pressure. The need for albumin in the world reaches 500 tons annually, which is used hypoalbuminemia therapy. Hypoalbuminemia therapy which is often used is HSA (Human Serum Albumin) therapy. The use of HSA has disadvantages such as causing infections, expensive processing costs, and the cost of products. Thus other sources of albumin are needed. One that can be the potential source of albumin is Indonesian catfish (Clariasgariepinus). Our research studies have obtained the albumin from Indonesian catfish (C. gariepinus) and Patin fish (Pangasianodon hypophthalmus) with high yield and purity. The Indonesian catfish is the potential albumin source after the isolation and purification process was obtained that the molecular weight, the purity, and the total albumin content of purified albumin were 66.7 kDa, 95.38%, and 118.5 mg /g of wet weight, respectively.

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