Antibacterial Activity of Bee Venom Against Multidrug Resistance Staphylococcus Aureus From Milk of Cow and Buffaloes

Abstract

Bacteriological study includes bacteria isolated from Buffaloes milk, and Cow at Wassit Province was done in Technical institute. 120 milk samples were collected randomly from different places in Wassit Province included 60 samples from Buffaloes milk and 60 samples from Cow milk during the period October to December 2018. A bacteriological study was conducted for isolation and identification S. aureus by morphological and biochemical tests. The results showed that (20%) of Buffaloes milk and (13.33%) in Cow’s milk (13.33 %) of Cow milk contaminated with S. aureus. Anti-biogram pattern of  S. aureus  was carried out by using a diffusion method from an antibiotic saturated disk, Results showed more effect in S. aureus  isolated from Buffaloes milk was COT in efficiency ratio ( 78.33 % ), CLR in efficiency ratio ( 61 % ), SPX in efficiency ratio ( 51.66 % ), and L in efficiency ratio ( 15 % ). Also the results appeared more effective in S. aureus isolated from Cow milk was COT in efficiency ratio ( 56.66 % ), CLR in efficiency ratio ( 35 % ), SPX in efficiency ratio ( 30 % ), and L in efficiency ratio ( 8.33 % ), while S. aureus  appeared resistant in all milk samples from Buffaloes and Cow for antibiotic OX (100%), also MIC to staphylococcal ( 0.70 - 3.10 μg/ml ) While MBC concentration was (0.12-0.101 μg/ml).