Ameliorative effect of Cubeba Officinalis dried fruits against Tacrolimus induced nephrotoxicity in Wistar albino rats

Abstract

Cubeba Officinalis is traditionally effective in the treatment of various kidney ailments, and the main adverse effect of tacrolimus is nephrotoxicity. There is no documented evidence about the ameliorative potential of Cubeba Officinalis  in tacrolimus induced nephrotoxicity. The main endeavor of the study was to determine the nephroprotective activity of ethanolic extract of Cubeba Officinalis  dried fruits  against tacrolimus induced nephrotoxicity in Wistar albino rats. The Cubeba Officinalis  dried fruits were collected from the local market, and Male albino rats weighing 200-250 g were used for the study. The dose of 200 mg/kg as lower dose and the higher dose of 400mg/kg of test drug (EECO) was used, and silymarin is used as the standard at the dose of 20 mg/kg. The animals were divided into five groups, six animals each, which is started prior to oral administration of tacrolimus and continued with the fourteen days tacrolimus treatment. After the whole period of study, the rats were sacrificed, and histopathological studies and biochemical estimations were carried out. The BUN values were decreased from 33.60±3.84 in nephrotoxic rats to 28.27±2.48 (200mg/kg) and 20.70±0.81 (400mg/kg),creatinine levels from 1.645±0.21 to 0.926±0.19 (200mg/kg) and 0.638±0.07(400 mg/kg),uric acid levels from 1.822±0.249 to 1.092±0.306 (200 mg/kg) and 0.806±0.181 (400 mg/kg) sodium, potassium and chloride levels from 1.607± 0.091, 2.548± 0.293  and 259.8±6.42 to 1.302± 0.169 , 1.023±0.174 and 134.7±9.138 (200mg/kg of EECO) and 0.586±0.092 , 0.831±0.174 and 130.2±2.29 (400mg/kg of EECO).  The Ethanolic extract of cubeba officinalis was found to be effective in treating the nephrotoxicity in tacrolimus induced nephrotoxicity.