Umbilical cord derived mesenchymal stem cell proliferation in various platelet rich plasma and xeno-material containing medium

Ecie Budiyanti (1) , Isabella Kurnia Liem (2) , Jeanne Adiwinata Pawitan (3) , Dewi Wulandari (4) , Taufik Jamaan (5) , Kanadi Sumapradja (6)
(1) Biomedical Master program, Faculty of Medicine Universitas Indonesia, Jl. Salemba 6, Jakarta, Indonesia, Indonesia ,
(2) Stem Cell Medical Technology Integrated Service Unit, Cipto Mangunkusumo Central Hospital - Faculty of Medicine Universitas Indonesia, CMU 2 Building, 5th floor, Jl. Diponegoro 71, Jakarta, Indonesia / Department of Anatomy, Faculty of Medicine Universitas Indonesia, Jl. Salemba 6, Jakarta, Indonesia , Indonesia ,
(3) Stem Cell Medical Technology Integrated Service Unit, Cipto Mangunkusumo Central Hospital - Faculty of Medicine Universitas Indonesia, CMU 2 Building, 5th floor, Jl. Diponegoro 71, Jakarta, Indonesia / Department of Histology, Faculty of Medicine Universitas Indonesia, Jl. Salemba 6, Jakarta, Indonesia , Indonesia ,
(4) Department of Clinical Pathology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba 6, Jakarta, Indonesia , Indonesia ,
(5) Department of Obstetrics and Gynecology, Bunda Maternal and Child Hospital, Jl.Teuku Cik Ditiro 28, Central Jakarta, Jakarta, Indonesia, Indonesia ,
(6) Stem Cell Medical Technology Integrated Service Unit, Cipto Mangunkusumo Central Hospital - Faculty of Medicine Universitas Indonesia, CMU 2 Building, 5th floor, Jl. Diponegoro 71, Jakarta, Indonesia / Department of Obstetrics and Gynecology, Cipto Mangunkusumo Central Hospital, Jl. Diponegoro 71, Jakarta, Indonesia, Indonesia

Abstract

Previous studies used fetal bovine serum (FBS) containing medium for umbilical cord derived mesenchymal stem cell culture. Xenoproteins in FBS may be incorporated into the cultured cells and cause immune rejection, when the cells are used in patients. Therefore, the aim of this study was to compare propagation performance of umbilical cord derived stem cells that were cultured in various platelet rich plasma (PRP) containing media and FBS derivate containing commercial medium.This study was an in vitro analytical study on multiple harvest explant culture derived stem cells, which compared the stem cell population doubling time (PDT) of passage 1-3 cultures in human AB PRP containing media (low glucose and high glucose DMEM, and αMEM) compared to xenopretein containing commercial medium, MesenCult®. Overall, the lowest PDT was achieved by αMEM-PRP, and PDT in DMEM LG-PRP and αMEM PRP was comparable to those of MesenCult®. Features of cluster of differentiation (CD) expressions in P-1 suggest that the umbilical cord derived stem cells obtained from multiple harvest explant culture were not homogenous for MSCs. However, MSCs tended to become more homogenous with passages, especially for cells that were cultured in MesenCult®, followed by those cultured in αMEM-PRP. In conclusion, the best propagation performance of umbilical cord derived stem cells passage 1-3 cultures was achieved in PRP containing αMEM.

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Authors

Ecie Budiyanti
Isabella Kurnia Liem
Jeanne Adiwinata Pawitan
jeanneadiwip@fk.ui.ac.id (Primary Contact)
Dewi Wulandari
Taufik Jamaan
Kanadi Sumapradja
Ecie Budiyanti, Isabella Kurnia Liem, Jeanne Adiwinata Pawitan, Dewi Wulandari, Taufik Jamaan, & Kanadi Sumapradja. (2015). Umbilical cord derived mesenchymal stem cell proliferation in various platelet rich plasma and xeno-material containing medium. International Journal of Research in Pharmaceutical Sciences, 6(1), 7–13. Retrieved from https://ijrps.com/home/article/view/3891

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