Development and Validation of a HPLC Method for Determination and Quantification of α-­mangostin in Bark Extract of Garcinia cowa Roxb

Abstract

Kandis, Garcinia cowa Roxb, traditionally has been used for many purposes. Many parts of G. cowa have been used in traditional folk medicine as antipyretic and anti-­inflammatory. α-­mangostin represents the majority of the clinical  benefits  of  this  herbal  medicine.  It  is  reasonable  and  logical  to  determine  the  concentration  of  α-­mangostin as a chemical marker for the quality control of G. cowa and its products. The aim of this study was to set  up  a  validated  and  stability-­indicated  isocratic  reverse  phase  high  performance  liquid  chromatographic  (RP-­ HPLC) method for quality control and quantity determination of α-­mangostin from ethanol extract of G. cowa. The assay was fully validated and shown to be linear (r² = 0.999), sensitive (LOD = 0.04 μg/ml and LOQ = 0.16 μg/ml) and precise (intra-day variation ≤ 1.6 %, inter-­day variation ≤ 4.3%). Accuracy of the method was determined by a recovery study conducted at 3 different levels, and the average recovery was 86.67%. Total analysis was ~ 15 min. The  present  method  should  be  useful  for  analytical  research  and  for  routine  quality  control  analysis  of  α-­mangostin in ethanol extract of G. cowa.