Cytokine Mediated Immunomodulatory Properties of Kaempferol-5-O-β-D- glucopyranoside from methanol extract of aerial parts of Indigofera aspalathoides Vahl ex DC

Subramaniam Swarnalatha (1) , Ayarivan Puratchikody (2)
(1) Department of Pharmacology, Aadhi Bhagawan College of Pharmacy, Rantham, Cheyyar - 604 407, Tamil Nadu, India , India ,
(2) Department of Pharmaceutical Technology, Bharathidasan Institute of Technology, Anna University, Tiruchirappalli - 620 024, Tamil Nadu, India, India

Abstract

The objective of the study was to evaluate the immunomodulatory effect of isolate compound Kaempferol-5-O-β- D-glucopyranoside from methanol extract of aerial parts of Indigofera aspalathoides Vahl ex DC. on Swiss albino rats by Cyclophosphamide induced myelosuppression, In vivo phagocytic activity by carbon clearance assay, Pla- que forming cell (PFC) assay, flow cytometric studies for the estimation of T lymphocytes (CD3 and CD19) and Th1 cytokines (IL-2, IFN-γ and IL-4). Levamisole (50 mg/kg b.w.p.o) and Cyclophosphamide (30mg/kg b.w.i.p.) were used as immunostimulatory and immunosuppressive standard drugs. Administration of Kaempferol-5-O-β-D- glucopyranoside at the doses of 10, 20, 40, 50 mg/kg b. w. p. o. showed significant increase in the phagocytic in- dex and in Plaque forming cell assay, and an significant protection against cyclophosphamide induced neutropenia and showed dose dependent increase in the production of T lymphocytes (CD3 and CD19) and Th 1 cytokines (IL-2, IFN-γ and IL-4). The maximum increase in the production of CD3, CD19, IL-2, IFN-γ and IL-4 at 50 mg kg b.w of the test compound was observed. The above results suggest that Kaempferol-5-O-β-D-glucopyranoside from methanol extract of aerial parts of Indigofera aspalathoides Vahl ex DC. can be further explored for the development of potent immunomodulatory drug.

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Authors

Subramaniam Swarnalatha
Ayarivan Puratchikody
puratchikodypharma@gmail.com (Primary Contact)
Subramaniam Swarnalatha, & Ayarivan Puratchikody. (2014). Cytokine Mediated Immunomodulatory Properties of Kaempferol-5-O-β-D- glucopyranoside from methanol extract of aerial parts of Indigofera aspalathoides Vahl ex DC. International Journal of Research in Pharmaceutical Sciences, 5(1), 74–79. Retrieved from https://ijrps.com/home/article/view/3788

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