Enantiomeric separation of Midodrine hydrochloride in bulk and pharmaceutical dosage form by chiral HPLC

Jenifer Ashwini S (1) , Narenderan ST (2) , Meyyanathan SN (3) , Babu B (4) , Gowramma B (5)
(1) Department of Pharmaceutical Analysis, JSS College of Pharmacy, Udhagamandalam, JSS Academy of Higher Education & Research, Tamil Nadu, India, India ,
(2) Department of Pharmaceutical Analysis, JSS College of Pharmacy, Udhagamandalam, JSS Academy of Higher Education & Research, Tamil Nadu, India, India ,
(3) Department of Pharmaceutical Analysis, JSS College of Pharmacy, Udhagamandalam, JSS Academy of Higher Education & Research, Tamil Nadu, India, India ,
(4) Department of Pharmaceutical Analysis, JSS College of Pharmacy, Udhagamandalam, JSS Academy of Higher Education & Research, Tamil Nadu, India, India ,
(5) Department of Pharmaceutical Chemistry, JSS College of Pharmacy, Udhagamandalam, JSS Academy of Higher Education & Research, Tamil Nadu, India, India

Abstract

A simple, sensitive chiral liquid chromatographic method was developed for the separation and quantification of enantiomers Midodrine. A chiral PAK IG3 (150 x 4.6 mm) 3µm column was used for the separation of the enantiomers. The mobile phase consists of 10 mM ammonium bicarbonate in water and acetonitrile in the ratio of 95:5, v/v with a flow rate of 0.7 ml/min. The detection was done at 290 nm with column temperature maintained at 40°C. The method linear ranged between 10 – 110 μg/ml and 5 – 100 μg/ml for (+) and (-) Midodrine enantiomers. The recovery of the method was found to be in the range of 99.1 to 101.2 %. The detection limit for the (+) and (-) enantiomers was found to be 4 μg/ml and 1 μg/ml, respectively. A simple validated chiral HPLC method with reverse elusion is described for the separation and quantification of the enantiomers of Midodrine in bulk and formulation.

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Authors

Jenifer Ashwini S
Narenderan ST
Meyyanathan SN
thammababu@gmail.com (Primary Contact)
Babu B
Gowramma B
Jenifer Ashwini S, Narenderan ST, Meyyanathan SN, Babu B, & Gowramma B. (2019). Enantiomeric separation of Midodrine hydrochloride in bulk and pharmaceutical dosage form by chiral HPLC. International Journal of Research in Pharmaceutical Sciences, 10(3), 1608–1611. Retrieved from https://ijrps.com/home/article/view/3763

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