Antioxidant activity and Hepatoprotective potential of Ethanolic leaf extract of Artabotrys hexapetalus against various Hepatotoxins induced Hepatotoxicity in Albino wister Rats

Suresh K (1) , Hindustan Abdul Ahad (2) , Satyanarayana SV (3)
(1) Department of Pharmacognosy, Mother Theresa Institute of Pharmaceutical Education & Research (MIPER), Kurnool, Andhra Pradesh, India, India ,
(2) Department of Industrial Pharmacy, Raghavendra Institute of Pharmaceutical Education and Research (RIPER)- Autonomous, Ananthapuramu, Andhra Pradesh, India, India ,
(3) Department of Chemical Engineering, College of Engineering and Director, Academic and Planning, JNTU Anantapur, Andhra Pradesh, India, India

Abstract

The objective of this research was to see whether the ethanolic extract of Artabotrys hexapetalus leaves had antioxidant and hepatoprotective properties against paracetamol (PCT), ethanol (ETN), and isoniazid and rifampicin (IR)-induced hepatotoxicity in Albino Wister rats. The materials were dried in the shade, pulverised, and extracted using ethanol. Phytochemical experiments were carried out as a first step. The ethanol extract's hepatoprotective activity was evaluated in Albino Wister rats. PCT (3 g/kg), ETN (5 g/kg), and IR (100 mg/kg) reduced the levels of SGOT, SGPT, ALP, and bilirubin, which are all biochemical indicators of liver injury. Both hepatotoxin-treated and untreated group of animals determined for their antioxidant levels. Aspartate aminotransferase (SGOT), alanine transaminase (SGPT), alkaline phosphatase (ALP), bilirubin, an antioxidant function of DPPH (1,1-diphenyl 2-picryl hydroxyl), hydrogen peroxide (H2O2), lipid peroxidation methods, hydroxyl radicals, and nitric oxide were among the biochemical and histopathological tests performed. The altered levels of biochemical markers were restored to near-normal levels in a dose-dependent fashion after treatment with A. hexapetalus ethanolic leaf extract (100 mg/kg, 200 mg/kg, and 400 mg/kg body weight). The findings of the current research indicated that the ethanol leaf extract of A. hexapetalus had potent antioxidant and hepatoprotective properties.

Full text article

Generated from XML file

References

Acharya, K., Chatterjee, S., Biswas, G., Chatterjee, A., Saha, G. 2012. Hepatoprotective Effect of a wild edible mushroom on carbon tetrachloride- induced hepatotoxicity in mice. International Journal of Pharmacy and Pharmaceutical Sciences, 4:285–288.

Akhila, J., Manikkoth, S., Shyam, D., Alwar, M. 2007. Acute toxicity studies and determination of median lethal dose. Current Science, 93:917–920.

Annapurna, A., Ganapathi, S. 2016. Evaluation of hepatoprotective activity and oxidative stress parameters of alcoholic extract of Artabotryshexapetalus (L.f) Bhandari. Journal of Global Trends in Pharmaceutical Sciences, 7:3192–3199.

Armeni, T., Principato, G. 2020. Glutathione, an Over One Billion Years Ancient Molecule, Is Still Actively Involved in Cell Regulatory Pathways. The First Outstanding 50 Years of “UniversitàPolitecnicadelle Marche”, pages 417–429.

Dash, D.K., Yeligar, V.C., Nayak, S.S., Ghosh, T., Rajalingam, R., Sengupta, P., Maiti, B.C., Maity, T.K. 2007. Evaluation of the hepatoprotective and antioxidant activity of Ichnocarpus frutescens (Linn.) R. Br. on paracetamol-induced hepatotoxicity in rats. Tropical Journal of Pharmaceutical Research, 6(3):755–765.

David, B., Wolfender, J.-L., Dias, D.A. 2015. The pharmaceutical industry and natural products: historical status and new trends. Phytochemistry Reviews, 14(2):299–315.

Dhiman, A. 2012. A recent update in research on the antihepatotoxic potential of medicinal plants. Journal of Chinese Integrative Medicine, 10(2):117–127.

Haldar, P.K., Adhikari, S., Bera, S., Bhattacharya, S., Panda, S.P., Kandar, C.C. 2011. Hepatoprotective efficacy of Swietenia mahagoni L. Jacq. (Meli-aceae) bark against paracetamol-induced hepatic damage in rats. Indian Journal of Pharmaceutical Education and Research, 45(2):108–113.

Hoofnagle, J.H., Carithers, R.L.J., Shapiro, C., Ascher, N. 1995. Fulminant hepatic failure: summary of a workshop. Hepatology, 21(1):240–252.

Jindal, S.L. 2017. Flowering shrubs in India.1st Edition. Publications Division Ministry of Information & Broadcasting, pages 32–34.

King, J. 1965. The hydrolases-acid and alkaline phosphatases. Practical clinical enzymology, pages 121–122.

Larbie, C., Arthur, F.N., Woode, E., Terlabi, E. 2012. Evaluation of hepatoprotective effect of aqueous extract of Annona muricata (Linn.) leaf against carbon tetrachloride and acetaminophen-induced liver damage. Journal of Natural Pharmaceuticals, 3(1):25–25.

Malloy, HT., Evelyn, K.A. 1937. The determination of bilirubin with the photoelectric colorimeter. Journal of Biological Chemistry, 119(2):481– 490.

Manokaran, S., Jaswanth, A., Sengottuvelu, S., Nandhakumar, J., Duraisamy, R., Karthikeyan, D., Mallegaswari, R. 2008. Hepatoprotective activity of Aervalanata Linn. against paracetamol-induced hepatotoxicity in rats. Research journal of pharmacy and technology, 1(4):2008–2009.

Marinova, G., Batchvarov, V. 2011. Evaluation of the methods for determination of the free radical scavenging activity by DPPH. Bulgarian Journal of Agricultural Science, 17(1):11–24.

Mathew, J.V.K.G 2013. Artabotryszeylanicus var. kottavasaliyana (Annonaceae): a new variety from the southern Western Ghats. International Journal of Plant-Animal and Environmental Sciences, 3:153–155.

Meharie, B.G., Amare, G.G., Belayneh, Y.M. 2020. Evaluation of Hepatoprotective Activity of the Crude Extract and Solvent Fractions of Clutiaabyssinica (Euphorbiaceae) Leaf Against CCl 4-Induced Hepatotoxicity in Mice. Journal of Experimental Pharmacology, 12:137–150.

National Research Council 2010. Guide for the care and use of laboratory animals. National Academies Press. 8th edition, page: 1-246.

Prabhukumar, K.M., Robi, A.J., Hareesh, V.S., Dantas, K.J., Sujanapal, P., Indira, B. 2017. Artabotrys sahyadricus (Annonaceae), a new species from Western Ghats of India. Webbia, 72(1):117–120.

Reitman, S., Frankel, S. 1957. A Colorimetric Method for the Determination of Serum Glutamic Oxalacetic and Glutamic Pyruvic Transaminases. American Journal of Clinical Pathology, 28(1):56– 63.

Shahjahan, M., Sabitha, K.E., Jainu, M., Devi, C.S.S. 2004. Effect of Solanum trilobatum against carbon tetrachloride-induced hepatic damage in albino rats. The Indian Journal of Medical Research, 120(3):194–198.

Sroka, Z., Cisowski, W. 2003. Hydrogen peroxide scavenging, antioxidant and anti-radical activity of some phenolic acids. Food and Chemical Toxicology, 41(6):753–758.

Yadav, R.N.S., Agarwala, M. 2011. Phytochemical analysis of some medicinal plants. Journal of phytology, 3(2):10–14.

Zhang, C., Wang, N., Xu, Y., Tan, H.Y., Li, S., Feng, Y. 2018. Molecular Mechanisms Involved in Oxidative stress-associated Liver Injury Induced by Chinese Herbal Medicine: An Experimental Evidence-Based Literature Review and Network Pharmacology Study. International Journal of Molecular Sciences, 19(9).

Authors

Suresh K
Hindustan Abdul Ahad
abdulhindustan@gmail.com (Primary Contact)
Satyanarayana SV
Suresh K, Hindustan Abdul Ahad, & Satyanarayana SV. (2021). Antioxidant activity and Hepatoprotective potential of Ethanolic leaf extract of Artabotrys hexapetalus against various Hepatotoxins induced Hepatotoxicity in Albino wister Rats. International Journal of Research in Pharmaceutical Sciences, 12(2), 1679–1688. Retrieved from https://ijrps.com/home/article/view/348

Article Details

No Related Submission Found