Green tea phytocompounds targets Lansterol 14-α demethylase against ergosterol biosynthesis in Candida glabrata

Priyanka Sirari (1) , Jigisha Anand (2) , Devvret (3) , Ashish Thapliyal (4) , Nishant Rai (5)
(1) Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, Uttarakhand, India, India ,
(2) Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, Uttarakhand, India, India ,
(3) Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, Uttarakhand, India, India ,
(4) Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, Uttarakhand, India, India ,
(5) Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, Uttarakhand, India, India

Abstract

Green tea is credited as one of the world’s healthiest drinks with enriched antioxidants. It is known for its multi-beneficial health benefits against diabetes, blood pressure, hypertension, gastro-intestinal upset and is bestowed with significant antimicrobial potential. There are previous scientific evidence highlighting the antifungal potential of green tea and has identified it as a potential inhibitor of non-albicans Candida species. Lansterol 14-α demethylase (Erg 11) or CYP51 protein belongs to the cytochrome P450 monooxygenase (CYP) superfamily. Erg 11 is involved in ergosterol biosynthesis and has a significant role in azole drug resistance in Candida glabrata. The present study attempted to identify the inhibitory potential of green tea phytocompounds against inhibition of Erg 11 in Candida glabrata using bioinformatics tool viz., autodock vina software. Out of 15 green tea phytocompounds investigated, the study identified, Rutin (-10.5 kcal) Kaempferitrin (-9.4kcal), Epigallocatechin gallate (-10kcal), Epicatechin gallate (-8.7kcal), and Coumaroylquinic acid (-8.6kcal) acid as the potent phytocompounds which showed significant molecular interaction with Erg 11 in Candida glabrata. In attribution to the constant emergence of azole-resistant isolates, this preliminary analysis therefore, indicated the potential of green tea phytocompounds against inhibition of non-albicans Candida specific candidiasis. However, further, in vitro antimicrobial efficacy of these phytocompounds, the dose regime, drug likeliness, and cytotoxic analysis are required to be investigated and validated.

Full text article

Generated from XML file

References

Anand, J., Semwal, P., Gautam, P., Thapliyal, A., Rai, N. 2015. Prediction of novel drug targets in Ergosterol biosynthesis pathway: a proposed mechanism of anticandidal activity of green tea phytocompounds. Journal of Chemical and Pharmaceutical Research, 7(2):672–684.

Arendrup, M. C. 2013. Candida and candidaemia. Susceptibility and epidemiology. Danish Medical Journal, 60(11):B4698.

Arnold, K., Bordoli, L., Kopp, J., Schwede, T. 2006. The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling. Bioinformatics, 22(2):195–201.

Deorukhkar, S. C., Saini, S., Mathew, S. 2014. Non-albicans Candida Infection: An Emerging Threat. Interdisciplinary Perspectives on Infectious Diseases, 2014:1–7.

Du, H., Bing, J., Hu, T., Ennis, C. L., Nobile, C. J., Huang, G. 2020. Candida auris: Epidemiology, biology, antifungal resistance, and virulence. PLOS Pathogens, 16(10):e1008921.

Gasteiger, E., Hoogland, C., Gattiker, A., Duvaud, S., Wilkins, M. R., Appel, R. D., Bairoch, A. 2005. Protein identification and analysis tools on the ExPASy server. The proteomics protocols handbook, pages 571–607.

Geber, A., Hitchcock, C. A., Swartz, J. E., Pullen, F. S., Marsden, K. E., Kwon-Chung, K. J., Bennett, J. E. 1995. Deletion of the Candida glabrata ERG3 and ERG11 genes: effect on cell viability, cell growth, sterol composition, and antifungal susceptibility. Antimicrobial Agents and Chemotherapy, 39(12):2708–2717.

Lv, Q.-Z., Yan, L., Jiang, Y.-Y. 2016. The synthesis, regulation, and functions of sterols in Candida albicans: Well-known but still lots to learn. Virulence, 7(6):649–659.

Mishra, N., Prasad, T., Sharma, N., Payasi, A., Prasad, R., Gupta, D., Singh, R. 2007. Pathogenicity and drug resistance in Candida albicans and other yeast species. Acta Microbiologica et Immunologica Hungarica, 54(3):201–235.

Presterl, E., Daxböck, F., Graninger, W., Willinger, B. 2007. Changing pattern of candidaemia 2001–2006 and use of antifungal therapy at the University Hospital of Vienna, Austria. Clinical Microbiology and Infection, 13(11):1072–1076.

Rudramurthy, S. M., Chakrabarti, A., Paul, R. A., Sood, P., Kaur, H., Capoor, M. R., Kindo, A. J., Marak, R. S. K., Arora, A., Sardana, R., Das, S., Chhina, D., Patel, A., Xess, I., Tarai, B., Singh, P., Ghosh, A. 2017. Candida auris candidaemia in Indian ICUs: analysis of risk factors. Journal of Antimicrobial Chemotherapy, 72(6):1794–1801.

Villasmil, M. L., Barbosa, A. D., Cunningham, J. L., Siniossoglou, S., Nickels, J. T. J. 2020. An Erg11 lanosterol 14-α-demethylase-Arv1 complex is required for Candida albicans virulence. PLOS ONE, 15(7):e0235746.

Zhang, J., Li, L., Lv, Q., Yan, L., Wang, Y., Jiang, Y. 2019. The Fungal CYP51s: Their Functions, Structures, Related Drug Resistance, and Inhibitors. Frontiers in Microbiology, 10:691.

Zhou, Y., Liao, M., Zhu, C., Hu, Y., Tong, T., Peng, X., Li, M., Feng, M., Cheng, L., Ren, B., Zhou, X. 2018. ERG3 and ERG11 genes are critical for the pathogenesis of Candida albicans during the oral mucosal infection. International Journal of Oral Science, 10(2):9.

Authors

Priyanka Sirari
Jigisha Anand
Devvret
Ashish Thapliyal
Nishant Rai
nishantrai1@gmail.com (Primary Contact)
Priyanka Sirari, Jigisha Anand, Devvret, Ashish Thapliyal, & Nishant Rai. (2021). Green tea phytocompounds targets Lansterol 14-α demethylase against ergosterol biosynthesis in Candida glabrata. International Journal of Research in Pharmaceutical Sciences, 12(3), 1793–1797. Retrieved from https://ijrps.com/home/article/view/304

Article Details

No Related Submission Found