Assessment of Chromosome Cassette Pattern of MRSA in Correlation to Coagulase Gene

Salama Mostafa Abdel-Hafez (1) , Essam Hassan Mohamed (2) , Youssef Saeed Alghamdi (3) , Mohamed Mohamed Soliman (4) , Saad Hommod Alotaibi (5) , Adel Alkhedaide (6) , Magdy Yassin Hassan (7) , Hamada Hassan Amer (8)
(1) Department of Biology, Turabah University College, Taif University, Turabah-21995, Saudi Arabia, Saudi Arabia ,
(2) Department of Biology, Turabah University College, Taif University, Turabah-21995, Saudi Arabia, Saudi Arabia ,
(3) Department of Biology, Turabah University College, Taif University, Turabah-21995, Saudi Arabia, Saudi Arabia ,
(4) Cinical Laboratories Sciences, Turabah University College, Taif University, Turabah-21995, Saudi Arabia, Saudi Arabia ,
(5) Chemistry Department, Turabah University College, Turabah-29541, Taif University, Saudi Arabia, Saudi Arabia ,
(6) Cinical Laboratories Sciences, Turabah University College, Taif University, Turabah-21995, Saudi Arabia, Saudi Arabia ,
(7) Department of Biology, Turabah University College, Taif University, Turabah-21995, Saudi Arabia, Saudi Arabia ,
(8) Chemistry Department, Turabah University College, Turabah-29541, Taif University, Saudi Arabia, Saudi Arabia

Abstract

The resistance of methicillin-resistant Staphylococcus aureus (MRSA) to antimicrobials drugs is due to expression of the mecA gene. Current study was conducted on 33 MRSA clinical samples (Cefotaxime and Oxacillin positive). All MRSA isolates were examined using Antibiogram, Minimum Inhibitory concentration (MIC) and PCR to clarify the expression of SCCmec genes and to detect the differences on repeats of coagulase gene (Coa). Results showed that all isolates were 100% resistant against Amoxycillin-clavulanate, Ampicillin and Cefotaxime, 45.5% were resistant against Ciprofloxacin and erythromycin. mecA gene is expressed in all examined isolates (100%). All examined isolates harbored and expressed coagulase gene repeats. Coagulase repeats were 27.27% with 5 repeats (81pb), and 72.72% with 4 repeats. In conclusion, the virulence of MRSA strains is increased and gave different antibiogram activities from different global regions and the repeats of Coa gene give no detectable differences among MRSA strains.

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Authors

Salama Mostafa Abdel-Hafez
drsalamahafez@gmail.com (Primary Contact)
Essam Hassan Mohamed
Youssef Saeed Alghamdi
Mohamed Mohamed Soliman
Saad Hommod Alotaibi
Adel Alkhedaide
Magdy Yassin Hassan
Hamada Hassan Amer
Salama Mostafa Abdel-Hafez, Essam Hassan Mohamed, Youssef Saeed Alghamdi, Mohamed Mohamed Soliman, Saad Hommod Alotaibi, Adel Alkhedaide, Magdy Yassin Hassan, & Hamada Hassan Amer. (2021). Assessment of Chromosome Cassette Pattern of MRSA in Correlation to Coagulase Gene. International Journal of Research in Pharmaceutical Sciences, 12(3), 2207–2214. Retrieved from https://ijrps.com/home/article/view/257

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