Identification of Bacteria Sample Plaque from Periodontal Disease Among Diabetic Patients

Shahida Mohd-Said (1) , Nur Fatimah Zaharah Salehuddin (2) , Siti Nursyuhada Jafar (3) , Haslinda Ramli (4) , Tuti Ningseh Mohd-Dom (5) , Rohazila Mohamad Hanafiah (6)
(1) Faculty of Dentistry Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Federal Territory of Kuala Lumpur, Malaysia, Malaysia ,
(2) Faculty of Dentistry Universiti Sains Islam Malaysia, Persiaran MPAJ, Jalan Pandan Utama 55100 Pandan Indah, Ampang, Malaysia, Malaysia ,
(3) Faculty of Dentistry Universiti Sains Islam Malaysia, Persiaran MPAJ, Jalan Pandan Utama 55100 Pandan Indah, Ampang, Malaysia, Malaysia ,
(4) Faculty of Dentistry Universiti Sains Islam Malaysia, Persiaran MPAJ, Jalan Pandan Utama 55100 Pandan Indah, Ampang, Malaysia, Malaysia ,
(5) Faculty of Dentistry Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Federal Territory of Kuala Lumpur, Malaysia, Malaysia ,
(6) Faculty of Dentistry Universiti Sains Islam Malaysia, Persiaran MPAJ, Jalan Pandan Utama 55100 Pandan Indah, Ampang, Malaysia, Malaysia

Abstract

Molecular identification of bacteria are very important to develop database gene bank in university.  Databases of genes are very important to annotate a collection of nucleotide sequence and protein translation of oral bacteria. This research was conducted to identify bacteria that present in the plaque sample among diabetic patients collected from the Department of Periodontology and Community Oral health Universiti Sains Islam Malaysia (USIM). A total of bacteria was successfully isolated on Blood Agar from periodontal patients’ plaques. Colony Forming Unit (CFU/mL) was calculated to estimate the number of viable bacteria in a sample after performed serial dilution. Gram stained was done for all isolated samples to differentiate between Gram positive and negative organisms. About 2.07 x 107 CFU/mL of bacteria was successfully collected from periodontal plaques on diabetes patient. From the numbers, only 17 were selected to identify the species. Seven from the samples were Gram-positive bacteria, meanwhile ten sam- ples were identified as Gram-negative bacteria. PCR products were isolated from the samples by using polymerase chain reaction analysis. Streptococcus mutans, Streptococcus gordonii,Streptococcus oralis,Streptococcus sanguinis,Streptococcus agalactiae,Streptococcus lutetiensis,Streptococcus downei,, Aggregatibacter actinomycetemcomitans, Klebsiella pneumoniae,Enterobacter clocae,Salmonella enterica subs enterica, Enterobacter ludwigii, Enterobacter mori, Enterobacter roggen kampii, Enterobacter cloacae, Enterobacter tabaci and Klebsiella pneumoniae were identified from all samples via DNA sequencing.

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Authors

Shahida Mohd-Said
Nur Fatimah Zaharah Salehuddin
Siti Nursyuhada Jafar
Haslinda Ramli
Tuti Ningseh Mohd-Dom
Rohazila Mohamad Hanafiah
rohazila@usim.edu.my (Primary Contact)
Shahida Mohd-Said, Nur Fatimah Zaharah Salehuddin, Siti Nursyuhada Jafar, Haslinda Ramli, Tuti Ningseh Mohd-Dom, & Rohazila Mohamad Hanafiah. (2021). Identification of Bacteria Sample Plaque from Periodontal Disease Among Diabetic Patients. International Journal of Research in Pharmaceutical Sciences, 12(1), 366–373. Retrieved from https://ijrps.com/home/article/view/252

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