The anti-malignant activity of Macrotyloma uniƒlorum mediated green synthesized Cu and Zn metal-ligand nano complexes
The green biological route has been employed to convert macro-sized zinc and copper metal complexes into nano-sized metal complexes. These bioactive metal complexes were synthesized by template condensation process in the chemistry laboratory. The newly synthesized biologically active complexes were converted into nano range from phytochemical aqueous extract of Macrotyloma uniflorum (horse gram). Biologically converted nano complexes have been characterized by physicochemical as well as spectroscopic techniques such as UV-visible spectrophotometer and FTIR. The reduced Cu and Zn metal nanoparticles were analyzed with SEM for shape and size, which showed most of the nanoparticles, were nearly spherical with nano range size. To estimate the potentiality of newly manufactured copper and zinc nanoparticles in vitro and in in vivo studies, i.e. antifungal and antibacterial and anti-cancer activities were performed. The biogenic nanoparticles of Cu and Zn were evaluated for their activity on cancer A-549 cell lines by standard MTT assay for metabolically active mitochondria and cell viability. Further flow cytometric studies showed Cu, and Zn nano complexes had inhibition efficacy of cancer cells compared to normal cells. This study elevates that biosynthesized Cu & Zn nano complexes can be an alternative for the treatment of cancer.
Keywords:Green Nanoparticles, Macrotyloma uniflorum, SEM, Flow cytometry, Malignant, Lung Cancer
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