\documentclass[twocolumn,twoside]{article}
\makeatletter\if@twocolumn\PassOptionsToPackage{switch}{lineno}\else\fi\makeatother

\usepackage{amsfonts,amssymb,amsbsy,latexsym,amsmath,tabulary,graphicx,times,xcolor}

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
% Following additional macros are required to function some 
% functions which are not available in the class used.
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
\usepackage{url,multirow,morefloats,floatflt,cancel,tfrupee}
\makeatletter


\AtBeginDocument{\@ifpackageloaded{textcomp}{}{\usepackage{textcomp}}}
\makeatother
\usepackage{colortbl}
\usepackage{xcolor}
\usepackage{pifont}
\usepackage[nointegrals]{wasysym}
\urlstyle{rm}
\makeatletter

%%%For Table column width calculation.
\def\mcWidth#1{\csname TY@F#1\endcsname+\tabcolsep}

%%Hacking center and right align for table
\def\cAlignHack{\rightskip\@flushglue\leftskip\@flushglue\parindent\z@\parfillskip\z@skip}
\def\rAlignHack{\rightskip\z@skip\leftskip\@flushglue \parindent\z@\parfillskip\z@skip}

%Etal definition in references
\@ifundefined{etal}{\def\etal{\textit{et~al}}}{}


%\if@twocolumn\usepackage{dblfloatfix}\fi
\usepackage{ifxetex}
\ifxetex\else\if@twocolumn\@ifpackageloaded{stfloats}{}{\usepackage{dblfloatfix}}\fi\fi

\AtBeginDocument{
\expandafter\ifx\csname eqalign\endcsname\relax
\def\eqalign#1{\null\vcenter{\def\\{\cr}\openup\jot\m@th
  \ialign{\strut$\displaystyle{##}$\hfil&$\displaystyle{{}##}$\hfil
      \crcr#1\crcr}}\,}
\fi
}

%For fixing hardfail when unicode letters appear inside table with endfloat
\AtBeginDocument{%
  \@ifpackageloaded{endfloat}%
   {\renewcommand\efloat@iwrite[1]{\immediate\expandafter\protected@write\csname efloat@post#1\endcsname{}}}{\newif\ifefloat@tables}%
}%

\def\BreakURLText#1{\@tfor\brk@tempa:=#1\do{\brk@tempa\hskip0pt}}
\let\lt=<
\let\gt=>
\def\processVert{\ifmmode|\else\textbar\fi}
\let\processvert\processVert

\@ifundefined{subparagraph}{
\def\subparagraph{\@startsection{paragraph}{5}{2\parindent}{0ex plus 0.1ex minus 0.1ex}%
{0ex}{\normalfont\small\itshape}}%
}{}

% These are now gobbled, so won't appear in the PDF.
\newcommand\role[1]{\unskip}
\newcommand\aucollab[1]{\unskip}
  
\@ifundefined{tsGraphicsScaleX}{\gdef\tsGraphicsScaleX{1}}{}
\@ifundefined{tsGraphicsScaleY}{\gdef\tsGraphicsScaleY{.9}}{}
% To automatically resize figures to fit inside the text area
\def\checkGraphicsWidth{\ifdim\Gin@nat@width>\linewidth
	\tsGraphicsScaleX\linewidth\else\Gin@nat@width\fi}

\def\checkGraphicsHeight{\ifdim\Gin@nat@height>.9\textheight
	\tsGraphicsScaleY\textheight\else\Gin@nat@height\fi}

\def\fixFloatSize#1{}%\@ifundefined{processdelayedfloats}{\setbox0=\hbox{\includegraphics{#1}}\ifnum\wd0<\columnwidth\relax\renewenvironment{figure*}{\begin{figure}}{\end{figure}}\fi}{}}
\let\ts@includegraphics\includegraphics

\def\inlinegraphic[#1]#2{{\edef\@tempa{#1}\edef\baseline@shift{\ifx\@tempa\@empty0\else#1\fi}\edef\tempZ{\the\numexpr(\numexpr(\baseline@shift*\f@size/100))}\protect\raisebox{\tempZ pt}{\ts@includegraphics{#2}}}}

%\renewcommand{\includegraphics}[1]{\ts@includegraphics[width=\checkGraphicsWidth]{#1}}
\AtBeginDocument{\def\includegraphics{\@ifnextchar[{\ts@includegraphics}{\ts@includegraphics[width=\checkGraphicsWidth,height=\checkGraphicsHeight,keepaspectratio]}}}

\DeclareMathAlphabet{\mathpzc}{OT1}{pzc}{m}{it}

\def\URL#1#2{\@ifundefined{href}{#2}{\href{#1}{#2}}}

%%For url break
\def\UrlOrds{\do\*\do\-\do\~\do\'\do\"\do\-}%
\g@addto@macro{\UrlBreaks}{\UrlOrds}



\edef\fntEncoding{\f@encoding}
\def\EUoneEnc{EU1}
\makeatother
\def\floatpagefraction{0.8} 
\def\dblfloatpagefraction{0.8}
\def\style#1#2{#2}
\def\xxxguillemotleft{\fontencoding{T1}\selectfont\guillemotleft}
\def\xxxguillemotright{\fontencoding{T1}\selectfont\guillemotright}

\newif\ifmultipleabstract\multipleabstractfalse%
\newenvironment{typesetAbstractGroup}{}{}%

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%

\usepackage[authoryear]{natbib}



\newif\ifnokeywords\nokeywordsfalse

\makeatletter\input{size10-pointfive.clo}\makeatother%
\definecolor{kwdboxcolor}{RGB}{242,242,242}
\usepackage[hidelinks,colorlinks=true,allcolors=blue]{hyperref}
\linespread{1}                 
\def\floatpagefraction{0.8}

\usepackage[paperheight=11.69in,paperwidth=8.26in,top=1in,bottom=1in,left=1in,right=.75in,headsep=24pt]{geometry}

\usepackage{multirow-custom}

\makeatletter

\def\hlinewd#1{%
  \noalign{\ifnum0=`}\fi\hrule \@height #1%
  \futurelet\reserved@a\@xhline}
\def\tbltoprule{\hlinewd{1pt}\\[-14pt]}
\def\tblbottomrule{\noalign{\vspace*{6pt}}\hline\noalign{\vspace*{2pt}}}
\def\tblmidrule{\hline\noalign{\vspace*{2pt}}}



\let\@articleType\@empty
\let\@journalDoi\@empty
\let\@journalVolume\@empty
\let\@journalIssue\@empty
\let\@crossMarkLink\@empty
\let\@receivedDate\@empty
\let\@acceptedDate\@empty
\let\@revisedDate\@empty
\let\@publishedDate\@empty
\let\@copyrightYear\@empty
\let\@firstPage\@empty
\def\articleType#1{\gdef\@articleType{#1}}
\def\journalDoi#1{\gdef\@journalDoi{#1}}
\def\crossMarkLink#1{\gdef\@crossMarkLink{#1}}
\def\receivedDate#1{\gdef\@receivedDate{#1}}
\def\acceptedDate#1{\gdef\@acceptedDate{#1}}
\def\revisedDate#1{\gdef\@revisedDate{#1}}
\def\publishedDate#1{\gdef\@publishedDate{#1}}
\def\copyrightYear#1{\gdef\@copyrightYear{#1}}
\def\journalVolume#1{\gdef\@journalVolume{#1}}
\def\journalIssue#1{\gdef\@journalIssue{#1}}
\def\firstPage#1{\gdef\@firstPage{#1}}
\let\@issuelogo\@empty%
\let\@issuelogoex\@empty%
\def\issuelogo#1{\gdef\@issuelogo{#1}}
\def\issuelogoex#1{\gdef\@issuelogoex{#1}}


\def\author#1{%
  \gdef\@author{%
    \hskip-\dimexpr(\tabcolsep)\hskip5pt%
    \parbox{\dimexpr\textwidth-1pt}%
    {\fontsize{11}{13}\selectfont\raggedright #1}%
  }%
}


\usepackage{pharmascope-abs}
\usepackage{caption}
\usepackage{lastpage}
\usepackage{fancyhdr}
\usepackage[noindentafter,explicit]{titlesec}
\usepackage{fontspec}

\setmainfont[%
BoldFont=cambriab.ttf,%
ItalicFont=CAMBRIAI.TTF,%
BoldItalicFont=CAMBRIAZ.TTF]{Cambria.ttf}
\lefthyphenmin = 3

\def\title#1{%
  \gdef\@title{%
      \vspace*{-40pt}%
       \ifx\@articleType\@empty\else{\fontsize{10}{12}\scshape\selectfont\hspace{8pt}\@articleType\hfill\mbox{}\par\vspace{2pt}}\fi%
       \minipage{\linewidth}
         \hrulefill\\[-0.7pt]%%{\includegraphics[width=75pt,height=50pt]{ijrps_logo.png}}\hfill
         \mbox{~}\hspace{5pt}\parbox{.1\linewidth}{\IfFileExists{images/\@issuelogo.\@issuelogoex}{\includegraphics[width=79pt,height=61pt]{images/\@issuelogo.\@issuelogoex}}{\includegraphics[width=75pt,height=50pt]{ijrps_logo.png}}}\hfill
         \fcolorbox{kwdboxcolor}{kwdboxcolor}{\parbox{.792\linewidth}{%
          \begin{center}\fontsize{17}{17}\selectfont\scshape\vskip-7pt International Journal of Research in Pharmaceutical Sciences\hfill\end{center}%
          \vspace*{-10pt}\hspace*{4pt}{\fontsize{8}{9}\selectfont Published by JK Welfare \& Pharmascope Foundation\hfill  Journal Home Page: \href{www.ijrps.com}{\color{blue}\underline{\smash{www.ijrps.com}}}}\hspace*{4pt}\mbox{}}}%
       \par\vspace*{-1pt}\rule{\linewidth}{1.3pt}%
       \endminipage%
    \par\vspace*{9.2pt}\parbox{.98\linewidth}{\linespread{.9}\raggedright\fontsize{14}{17}\selectfont #1}%
    \vspace*{-8pt}%
  }
}


\setlength{\parindent}{0pt}
\setlength{\parskip}{0.4pc plus 1pt minus 1pt}

\def\abbrvJournalTitle{Int. J. Res. Pharm. Sci.}

\fancypagestyle{headings}{%
  \renewcommand{\headrulewidth}{0pt}%
  \renewcommand{\footrulewidth}{0.3pt}
  \fancyhf{}%
  \fancyhead[R]{%
    \fontsize{9.12}{11}\selectfont\RunningAuthor,\ \abbrvJournalTitle,\ \ifx\@journalVolume\@empty X\else\@journalVolume\fi%
    \ifx\@journalIssue\@empty\else(\@journalIssue)\fi%
    ,\ \ifx\@firstPage\@empty 1\else\@firstPage\fi-\pageref*{LastPage}%
  }%
  \fancyfoot[LO,RE]{\fontsize{9.12}{11}\selectfont\textcopyright\ International Journal of Research in Pharmaceutical Sciences}%
  \fancyfoot[RO,LE]{\fontsize{9.12}{11}\selectfont\thepage}
}\pagestyle{headings}

                                                         
\fancypagestyle{plain}{%
  \renewcommand{\headrulewidth}{0pt}%
  \renewcommand{\footrulewidth}{0.3pt}%
  \fancyhf{}%
  \fancyhead[R]{%
    \fontsize{9.12}{11}\selectfont\RunningAuthor,\ \abbrvJournalTitle,\ \ifx\@journalVolume\@empty X\else\@journalVolume\fi%
    \ifx\@journalIssue\@empty\else(\@journalIssue)\fi%
    ,\ \ifx\@firstPage\@empty 1\else\@firstPage\fi-\pageref*{LastPage}%
  }%
  \fancyfoot[LO,RE]{\fontsize{9.12}{11}\selectfont\textcopyright\ International Journal of Research in Pharmaceutical Sciences}%
  \fancyfoot[RO,LE]{\fontsize{9.12}{11}\selectfont\thepage}
  \ifx\@firstPage\@empty\else\setcounter{page}{\@firstPage}\fi
}


\def\NormalBaseline{\def\baselinestretch{1.1}}
\usepackage{textcase}
\setcounter{secnumdepth}{0}

\titleformat{\section}[block]{\bfseries\boldmath\NormalBaseline\filright\fontsize{10.5}{13}\selectfont}
{\thesection}
{6pt}
{\MakeTextUppercase{#1}}
[]
\titleformat{\subsection}[block]{\bfseries\boldmath\NormalBaseline\filright\fontsize{10.5}{12}\selectfont}
{\thesubsection}
{6pt}
{#1}
[]
\titleformat{\subsubsection}[block]{\NormalBaseline\filright\fontsize{10.5}{12}\selectfont}
{\thesubsubsection}
{6pt}
{#1}
[]
\titleformat{\paragraph}[block]{\NormalBaseline\filright\fontsize{10.5}{10}\selectfont}
{\theparagraph}
{6pt}
{#1}
[]
\titleformat{\subparagraph}[block]{\NormalBaseline\filright\fontsize{10.5}{12}\selectfont}
{\thesubparagraph}
{6pt}
{#1}
[]

\titlespacing{\section}{0pt}{.5\baselineskip}{.5\baselineskip}  
\titlespacing{\subsection}{0pt}{.5\baselineskip}{.5\baselineskip}  
\titlespacing{\subsubsection}{0pt}{.5\baselineskip}{.5\baselineskip}  
\titlespacing{\paragraph}{0pt}{.5\baselineskip}{.5\baselineskip}  
\titlespacing{\subparagraph}{0pt}{.5\baselineskip}{.5\baselineskip}

\captionsetup[figure]{skip=1.4pt,font=bf,labelsep=colon,justification=raggedright,singlelinecheck=false}
\captionsetup[table]{skip=1.4pt,font=bf,labelsep=colon,justification=raggedright,singlelinecheck=false}

\def\bibyear#1{#1}
%\def\bibjtitle#1{#1}   %%Publisher request
\def\bibauand{}
\setlength\bibsep{3pt}
\setlength\bibhang{8pt}

\makeatother
\date{}


\usepackage{float}

\newfloat{scheme}{tbp}{Scheme}
\floatname{scheme}{Scheme}
\makeatletter
\@ifpackageloaded{endfloat}{\@ifpackagewith{endfloat}{figuresonly}{\DeclareDelayedFloat{scheme}{Scheme}}{\@ifpackagewith{endfloat}{tablesonly}{}{\DeclareDelayedFloat{scheme}{Scheme}}}}{}\makeatother

\begin{document}


\def\RunningAuthor{Madan Kumar Shankar et al.}
\firstPage{1375}
\articleType{Original Article}
\receivedDate{08 Feb 2021}
\acceptedDate{03 Apr 2021}
\revisedDate{04 Mar 2021}
\journalVolume{2021, 12}
\journalIssue{2}
\journalDoi{ijrps.v12i2.4693}
\copyrightYear{2021}
\def\authorCount{5}
\def\affCount{4}

\def\journalTitle{International Journal of Research in Pharmaceutical Sciences}

\title{Antioxidant studies of 3-(5-(2,4-dichlorophenoxy)-3-methyl-1-phenyl-1H-pyrazole-4-yl)-1-(furan-2-yl)prop-2-en-1-one}
\author{Ganesh~Somashekarachar\textsuperscript{1},  
            Arpita~Goswami\textsuperscript{2},  
            Kannika~Byadarahalli~Ravindranath\textsuperscript{3},  
            Madan~Kumar~Shankar\textsuperscript{*}\textsuperscript{3}, 
            Manju~Nagaraja\textsuperscript{4}~\\[5pt]{\textsuperscript{1}Department of Prosthodontics, JSS Dental College and Hospital, JSS Academy Of Higher Education and Research, Mysuru-570023, Karnataka, India}~\\{\textsuperscript{2}Department of Structural biology, Centre for DNA Fingerprinting and Diagnostics, Hyderabad-500001, Karnataka, India}~\\{\textsuperscript{3}Department of IOE, Vijnana Bhavan, University of Mysore, Mysore-570006, Karnataka, India }~\\{\textsuperscript{4}Department of Chemistry, Govt. Science College, Hassan, Karnataka, India}}

\begin{abstract}
Chalcones are flavonoid precursors, abundant in edible plants. The pyrazole moieties in chalcones show various pharmacological activities. Notably, Chalcone-based structures exhibit numerous pharmacological activities like antiinflammatory, antibacterial, antitumor, antioxidant functions because of having \ensuremath{\alpha }, \ensuremath{\beta }-unsaturated ketone moiety. Such broad spectrum biological activities were also observed in several nitrogen containing heterocycles. Among the heterocyclic systems, pyrazole stand unique. All these observations led us to design and synthesize pyrazole fused hybrid thiophenechalcone molecule 3-(5-(2,4-dichlorophenoxy)-3-methyl-1-phenyl-1H-pyrazole-4-yl)-1-(furan-2-yl)prop-2-en-1-one (3a). The synthesis was performed using base catalyzed reaction of pyrazole aldehyde and acetyl furan. Further characterization of the molecule was done by spectroscopy and antioxidant studies.  Spectroscopic methods included Fourier transform infrared (FTIR), nuclear magnetic resonance (NMR) and mass spectra (MS). The antioxidant potency of the compound was carried out based on the scavenging activity of 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH). This compound showed moderate free radical scavenging activity of 45.12 \%. 
\end{abstract}\def\keywordstitle{Keywords}

\begin{keywords}Chalcones,\newline Pyrazoles,\newline FTIR,\newline NMR,\newline antioxidant activity
\end{keywords}
\twocolumn[ \maketitle {\printKwdAbsBox}]
\makeatletter{\raggedright\textsuperscript{*}Corresponding Author\par Name:\ Madan~Kumar~Shankar~\\ Phone:\ ~\\  Email:\ madan.mx@gmail.com
        \par}\vspace*{-11pt}\hrulefill\par{\fontsize{12}{14}\selectfont ISSN: 0975-7538}\par% 
        \textsc{DOI:}\ \href{https://doi.org/10.26452/\@journalDoi}{\textcolor{blue}{\underline{\smash{https://doi.org/10.26452/\@journalDoi}}}}\par%
        \vspace*{-11pt}\hrulefill\\{\fontsize{9.12}{10.12}\selectfont Production and Hosted by}\par{IJRPS |~\href{www.ijrps.com}{www.ijrps.com}}\par%
        \vspace*{-2pt}{\fontsize{9.12}{10.12}\selectfont\textcopyright\ \@copyrightYear\ $|$ All rights reserved.}\par%
        \vspace*{-11pt}\rule{\linewidth}{1.2pt}
        \makeatother
        
    
\section{Introduction}
Chalcones are present in many edible plants and are the precursors of flavonoids and isoflavonoids. These consist of two aromatic rings are joined by a three-carbon \ensuremath{\alpha }, \ensuremath{\beta }-unsaturated carbonyl system. There are reports with chalcone-based structure exhibit a variety of pharmacological activities such as anti-inflammatory \unskip~\citep{1053311:21818139}, antibacterial \unskip~\citep{1053311:21818138}, antitumor \unskip~\citep{1053311:21818140}, antioxidant \unskip~\citep{1053311:21818141}, and these activity are largely attributed due to the \ensuremath{\alpha }, \ensuremath{\beta }-unsaturated ketone moiety. The nitrogen-containing heterocycles also exhibited a broad spectrum of biological activities in the field of medicinal chemistry. Among the heterocyclic system, pyrazole stands unique and exhibited varied pharmacological activity such as anti-inflammatory \unskip~\citep{1053311:21818143}, antibacterial \unskip~\citep{1053311:21818142}, antitumor \unskip~\citep{1053311:21818174}, antioxidant \unskip~\citep{1053311:21818393} and anti-fungal \unskip~\citep{1053311:21818166}. 

Prompted by these observations, we have designed pyrazole fused thiophene chalcone, synthesized, characterized using using spectroscopic (FT-IR, NMR, MS), and antioxidant studies were performed.


\bgroup
\fixFloatSize{images/6ffe4148-aa74-4a9a-a4f5-b7510c03c42f-upicture1.png}
\begin{scheme}[!htbp]
\centering \makeatletter\IfFileExists{images/6ffe4148-aa74-4a9a-a4f5-b7510c03c42f-upicture1.png}{\includegraphics{images/6ffe4148-aa74-4a9a-a4f5-b7510c03c42f-upicture1.png}}{}
\makeatother 
\caption{\boldmath {Reaction route of the title compound}}
\label{f-e92e88423ffc}
\end{scheme}
\egroup

\bgroup
\fixFloatSize{images/c809c60f-5e3a-4643-9e19-253fb05a8fd4-upicture2.png}
\begin{figure}[!htbp]
\centering \makeatletter\IfFileExists{images/c809c60f-5e3a-4643-9e19-253fb05a8fd4-upicture2.png}{\includegraphics{images/c809c60f-5e3a-4643-9e19-253fb05a8fd4-upicture2.png}}{}
\makeatother 
\caption{\boldmath {IR Spectrum of the title compound}}
\label{f-ca751c237843}
\end{figure}
\egroup

\bgroup
\fixFloatSize{images/ce516599-226f-47f4-9125-7036a0179cb7-upicture3.png}
\begin{figure}[!htbp]
\centering \makeatletter\IfFileExists{images/ce516599-226f-47f4-9125-7036a0179cb7-upicture3.png}{\includegraphics{images/ce516599-226f-47f4-9125-7036a0179cb7-upicture3.png}}{}
\makeatother 
\caption{\boldmath {\ensuremath{^{I}}H NMR Spectrum of the title compound}}
\label{f-ee908047ae27}
\end{figure}
\egroup

\bgroup
\fixFloatSize{images/d2cc7d92-cbba-4c86-96af-5875b5e32cdb-upicture4.png}
\begin{figure}[!htbp]
\centering \makeatletter\IfFileExists{images/d2cc7d92-cbba-4c86-96af-5875b5e32cdb-upicture4.png}{\includegraphics{images/d2cc7d92-cbba-4c86-96af-5875b5e32cdb-upicture4.png}}{}
\makeatother 
\caption{\boldmath {Free radical scavenging activity of the title compound}}
\label{f-d23f260524d6}
\end{figure}
\egroup

    
\section{Materials and Methods}
All reagents and solvents purchased commercially are used directly. IR spectra were recorded on a Shimadzu FT-IR spectrometer. \ensuremath{^{13}}C NMR was recorded on a BRUKER ADVANCE {\textendash} II 400 NMR Spectrometer.

\textbf{Synthesis}

The aetyl furan (2) (10 mmol) and pyrazole aldehyde (1) (10 mmol) are mixed in ethanol (25 mL). To this mixture, dropwise potassium hydroxide (5 mL, 30\%) was added under an ice bath. The reaction mixture was stirred for 4 hours, and the solid is obtained. The resultant solid was washed with water. The resultant solid is recrystallized using ethanol. And the reaction scheme is shown in Scheme~\ref{f-e92e88423ffc},\unskip~\citep{1053311:21818168}.
    
\section{Results and Discussion}
Spectroscopy M.P: 166-167 \ensuremath{^{o}}C, Yield: 73 \%

Mass Spectrometry

Molecular mass of the compound was determined by mass spectra and was recorded using a triple TIF 5600 Sciex mass spectrometer in both ESI positive and negative modes. \ensuremath{^{}} Mass (m/z) 439/ 441 (M.F. C\ensuremath{_{23}}H\ensuremath{_{16}}Cl\ensuremath{_{2}}N\ensuremath{_{2}}O\ensuremath{_{3}}) (M\ensuremath{^{+}} + 1)/ (M\ensuremath{^{+}} + 3).

FT-IR Spectroscopy (Figure~\ref{f-ca751c237843})- IR absorptions measured in ATR mode. IR (\ensuremath{\upsilon}\ensuremath{_{max}}, cm\ensuremath{^{-1}}): 1328 (C-O-C), 1512 (C=C), 1564 (C=N), 1671 (C=O) and 2918 (C-H).

\ensuremath{^{1}}H NMR and \ensuremath{^{13}}C NMR observations were shown in Figure~\ref{f-ee908047ae27}. For \ensuremath{^{1}}H NMR spectrum at 400 MHz, CDCl\ensuremath{_{3\ }} \ensuremath{\delta } ppm, peaks were observed: 7.59 (m, 4H, C3-H of chalcone \& 3H of Ar-H), 7.39 (m, 3H, C5-H of furan \& 2H of Ar-H), 7.26 (m, 1H, Ar-H), 7.14 (d, J= 3.40 Hz, 1H, C2-H furan proton), 7.08 (d, J= 15.96 Hz, 1H, C3-H, chalcone proton), 7.00 (dd, J=2.52 Hz, J=8.88 Hz, 1H, C5-H 2,4-di chloro phenyl), 6.52 ( m, 2H Ar-H) and 2.52 (s, 3H, -CH\ensuremath{_{3}} pyrazole). For \ensuremath{^{13}}C NMR spectrum at 100 MHz, CDCl\ensuremath{_{3}} \ensuremath{\delta } ppm, peaks were observed: 177.5 (C=O), 145.7 (C=N), 111.2-143.7 (Aromatic carbons), 14.11 (CH\ensuremath{_{3}}).

\textbf{Antioxidant Activity}

The concept of the scavenging activity of DDPH was used to quantify free radical scavenging activity for the compound. The stock solution (150\ensuremath{\mu}g/mL) of a compound and standard butylated hydroxyanisole (BHA) was taken in different test tubes. The MeOH was adjusted to make 1mL volume in test tubes. DPPH solution (1mL of 0.1 mM) was added in each test tube. These test tubes are vortexed and kept in the dark for 30 min, and they're (stable DPPH radical) showed absorbance at 517 nm. The control (DPPH, without sample) was prepared using the same procedure. The reported literature is used to calculate the radical scavenging activity \unskip~\citep{1053311:21818171}. 

$DPPH\;radical\;scavenging\;activity\;(\%)=\frac{(Abs\;Control\;-Abs\;Sample)}{Abs\;Control}\times100 $

 Where absorbance of DPPH radical + solvent (Methanol) is Abs Control, the absorbance of DPPH radical + test sample/standard BHA is Abs Sample. Synthesized compounds exhibited moderate radical scavenging activity. Pyrazole carrying furan (3a) showed 45.12 \% DPPH radical scavenging activity (Figure~\ref{f-d23f260524d6}). 
    
\section{Conclusions}
The compound 3-(5-(2,4-dichlorophenoxy)-3-methyl-1-phenyl-1H-pyrazole-4-yl)-1-(furan-2-yl)prop-2-en-1-one (3a) is prepared using base-catalyzed reaction of pyrazole aldehyde and acetyl furan. Spectroscopic methods (FTIR, NMR and mass spectra) were carried out to confirm the molecular structure of 3a. The antioxidant activity of the compound was carried out based on the scavenging activity of DPPH, and this compound moderate scavenging activity of 45.12 \%. 

\textbf{Acknowledgement}

Authors thank IOE, Vijnana Bhavan, University of Mysore, Mysuru and JSS Academy of Higher Education and Research, Mysuru 570023. 

\textbf{Funding Support}

The authors declare that they have no funding support for this study.

\textbf{Conflict of Interest}

The authors declare that they have no conflict of interest for this study.
    

\bibliographystyle{pharmascope_apa-custom}

\bibliography{\jobname}
\end{document}
