In-Vitro CT-DNA Cleavage of Newly Synthesized Zn(II) Complex with P-Mercaptoaniline and Benzoate Ion

Sudhakar P (1) , Rajasekar K (2) , Balasubramaniyan S (3) , Selvarani R (4) , Veeravel C (5) , Narasimhavarman S (6)
(1) Research Department of Chemistry, Government Arts College (Affiliated to Bharathidasan University, Tiruchirappalli-620024), Ariyalur – 621 713, Tamil Nadu, India, India ,
(2) Research Department of Chemistry, Government Arts College (Affiliated to Bharathidasan University, Tiruchirappalli-620024), Ariyalur – 621 713, Tamil Nadu, India, India ,
(3) Research Department of Chemistry, Government Arts College (Affiliated to Bharathidasan University, Tiruchirappalli-620024), Ariyalur – 621 713, Tamil Nadu, India, India ,
(4) Research Department of Chemistry, Government Arts College (Affiliated to Bharathidasan University, Tiruchirappalli-620024), Ariyalur – 621 713, Tamil Nadu, India, India ,
(5) Research Department of Chemistry, Government Arts College (Affiliated to Bharathidasan University, Tiruchirappalli-620024), Ariyalur – 621 713, Tamil Nadu, India, India ,
(6) Research Department of Chemistry, Government Arts College (Affiliated to Bharathidasan University, Tiruchirappalli-620024), Ariyalur – 621 713, Tamil Nadu, India, India

Abstract

Coordination complex of Zn(II) with 4-mercaptoaniline(4-MTP or 4-ATP) were synthesized under microwave irradiation. The synthesized complex was characterized by elemental analysis, metal estimation, UV-Visible, conductance measurement, IR, Far-IR & NMR (proton and carbon) spectral studies. The metal complex was found to mononuclear, neutral and colorless in nature which are confirmed by the elemental analysis and molar conductance studies. The geometry for 1:2:2 complex exhibits to be square planar were confirmed by its spectral absorption study. The ligand was coordinated to the metal ion through its ‘N’ or ‘S’ atom and diamagnetic nature of the complex to be confirmed by the IR, Far-IR and NMR spectral studies. The electrochemical properties of complex shows the well redox properties of them with Zn(II)/Zn(I) couple by predicting the values of anodic and cathodic current. In-vitro antibacterial and antifungal activities of microorganisms viz., S. Aureus, bacillus and C. Albicance were carried out by Agar disc diffusion method using Amikacin and ketoconazole as standard control and DMSO as solvent control. The metal complex shows enhanced biological activities than the 4-ATP. The CT-DNA cleavage study of 4-ATP and its Zn(II) complex were performed and calculated the IC50 values. The results show the antioxidant property of 4-ATP and its complex.

Full text article

Generated from XML file

References

Alaghaz, A.-N. M. A., Zayed, M. E., Alharbi, S. A., Ammar, R. A. A., Chinnathambi, A. 2015. Synthesis, spectroscopic identification, thermal, potentiometric and antibacterial activity studies of 4-amino-5-mercapto-S-triazole Schiff’s base complexes. Journal of Molecular Structure, 1087:60–67.

Aziz, A. A., Elantabli, F. M., Moustafa, H., El-Medani, S. M. 2017. Spectroscopic, DNA binding ability, biological activity, DFT calculations and non linear optical properties (NLO) of novel Co (II), Cu (II), Zn (II), Cd (II) and Hg(II) complexes with ONS Schiff base. Journal of Molecular Structure, 1141:563–576.

Chen, Y., Liu, X. M., Wu, X., Liu, X. C., Dong, W. H., Han, B. K., Chen, Q. 2017. An array of poly-l-histidine functionalized multi-walled carbon nanotubes on 4-aminothiophenol self-assembled monolayer and the application for sensitively glucose sensing. Electrochimica Acta, 258:988–997.

Denizot, F., Lang, R. 1986. Rapid colorimetric assay for cell growth and survival: Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. Journal of Immunological Methods, 89(2):271–277.

El-Maali, N. A., Osman, A. H., Aly, A. A. M., Al-Hazmi, G. A. A. 2005. Voltametric analysis of Cu (II), Cd (II) and Zn (II) complexes and their cyclic voltammetry with several cephalosporin antibiotics. Bioelectrochemistry, 65(2):95–104.

Faniran, J. A., Patel, K. S., Nelson, L. O. 1976. Physicochemical studies of metal β-diketonates-I Infrared spectra of 1-(3-pyridyl)-1,3-butanedione and its divalent metal complexes. Journal of Inorganic and Nuclear Chemistry, 38(1):77–80.

He, Y., Zhong, C., Zhou, Y., Zhangj, H. 2009. Synthesis and luminescent properties of novel Cu (II), Zn (II) polymeric complexes based on 1, 10-phenanthroline and biphenyl groups. Chem. Sci., 121(4):407–412.

Kumar, K. A., Reddy, K. L., Satyanarayana, S. 2010. Synthesis, DNA interaction and photocleavage studies of ruthenium (II) complexes with 2-(pyrrole) imidazo [4, 5-f]-1,10-phenanthroline as an intercalative ligand. Transition Metal Chemistry, 35(6):713–720.

Lanjekar, K. J., Rathod, V. K. 2021. Microwave catalysis in organic synthesis. Green Sustainable Process for Chemical and Environmental Engineering and Science, pages 1–50.

Li, F., Chen, W., Dong, P., Zhang, S. 2009. A simple strategy of probe DNA immobilization by diazotization-coupling on self-assembled 4-aminothiophenol for DNA electrochemical biosensor. Biosensors and Bioelectronics, 24(7):2160– 2164.

Parekh, J., Inamdhar, P., Nair, R., Baluja, S., Chanda, S. 2005. Synthesis and antibacterial activity of some Schiff bases derived from 4-aminobenzoic acid. Journal of the Serbian Chemical Society, 70(10):1155–1161.

Raman, N., Raja, J. D., Sakthivel, A. 2008. Template synthesis of novel 14-membered tetraazamacro-cyclic transition metal complexes: DNA cleavage and antimicrobial studies. Journal of the Chilean Chemical Society, 53(3):1568–1571.

Shafaatian, B., Mousavi, S. S., Afshari, S. 2016. Synthesis, characterization, spectroscopic and theoretical studies of new zinc (II), copper (II) and nickel (II) complexes based on imine ligand containing 2-aminothiophenol moiety. Journal of Molecular Structure, 1123:191–198.

Silva, J. F., Pavez, J., Silva, C. P., Zagal, J. H. 2013. Electrocatalytic actvity of modified gold electrodes based on self-assembled monolayers of 4-mercaptopyridine and 4-aminothiophenol on Au (111) surfaces chemically functionalized with substituted and unsubstituted iron phthalocyanines. Electrochimica Acta, 114:7–13.

Sitlani, A., Long, E. C., Pyle, A. M., Barton, J. K. 1992. DNA photocleavage by phenanthrenequinone diimine complexes of rhodium (III): shape-selective recognition and reaction. Journal of the American Chemical Society, 114(7):2303–2312.

Taha, R. H., El-Shafiey, Z. A., Salman, A. A., El-Fakharany, E. M., Mansour, M. M. 2018. Synthesis and Characterization of newly synthesized Schiff base ligand and its metal complexes as potent anti-cancer. Journal of Molecular Structure, 1181:536–545.

Tas, E., Kilic, A., Durgun, M., Küpecik, L., Yilmaz, I., Arslan, S. 2010. Cu (II), Co (II), Ni (II), Mn (II), and Fe (II) metal complexes containing N, N’-(3,4-diaminobenzophenon)-3,5-Bu(t)(2)- salicylaldimine ligand: Synthesis, structural characterization, thermal properties, electro chemistry, and spectroelectrochemistry. Spectrochim Acta A Mol Biomol Spectrosc, 75(2):811–818.

Yilmaz, I., Temel, H., Alp, H. 2008. Synthesis, electrochemistry and in situ spectroelectrochemistry of a new Co (III) thio Schiff-base complex with N, N′-bis(2-aminothiophenol)-1, 4-bis (carboxylidene phenoxy) butane. Polyhedron, 27(1):125–132.

Yilmaz, Y. Y., Yalcinkaya, E. E., Demirkol, D. O., Timur, S. 2020. 4-aminothiophenol-intercalated montmorillonite: Organic-inorganic hybrid material as an immobilization support for biosensors. Sensors and Actuators B: Chemical, 307:127665.

Zhang, N., Fan, Y., Zhang, Z., et al. 2012. Syntheses, crystal structures and anticancer activities of three novel transition metal complexes with Schiff base derived from 2-acetylpyridine and l-tryptophan. Inorganic Chemistry Communications, 22:68–72.

Authors

Sudhakar P
sudhakar9715@gmail.com (Primary Contact)
Rajasekar K
Balasubramaniyan S
Selvarani R
Veeravel C
Narasimhavarman S
Sudhakar P, Rajasekar K, Balasubramaniyan S, Selvarani R, Veeravel C, & Narasimhavarman S. (2021). In-Vitro CT-DNA Cleavage of Newly Synthesized Zn(II) Complex with P-Mercaptoaniline and Benzoate Ion. International Journal of Research in Pharmaceutical Sciences, 12(3), 1950–1955. Retrieved from https://ijrps.com/home/article/view/145

Article Details

No Related Submission Found