Evaluation of antioxidant property and anticancer prospective of the leaf extract and biofractions of Bauhinia foveolata Dalzell – A native of the Indian Western Ghats

Authors

  • Liesl Maria Fernandes e Mendonça Department of Pharmacology, Goa College of Pharmacy, 18th June Road, Panaji, Goa – 403001, India
  • Arun B Joshi Department of Pharmacognosy and Phytochemistry, Goa College of Pharmacy, 18th June Road, Panaji, Goa – 403001, India
  • Anant V Bhandarkar Department of Pharmacognosy and Phytochemistry, Goa College of Pharmacy, 18th June Road, Panaji, Goa – 403001, India
  • Himanshu Joshi Department of Pharmacology, College of Pharmacy, Graphic Era Hill University, Bhimtal campus, Uttarakhand – 263156, India

Abstract

The current study was designed to evaluate the antioxidant and anticancer potential of ethanolic leaf extract of Bauhinia foveolata Dalzell. (EEBF) and its toluene, ethyl acetate and methanolic biofractions viz., TFBF, EFBF and MFBF. Phytoconstituents were screened by adopting established procedures. Total phenolic and flavonoid content were assessed spectrophotometrically. In vitro antioxidant activity was assayed using DPPH (2,2-diphenyl-1-picrylhydrazyl), hydrogen peroxide and nitric oxide as free radicals, whereas anticancer activity was evaluated using sulforhodamine B assay. EEBF showed maximum phenolic content of 49.12±0.31 mg GAE/g and flavonoidal content of 28.75±0.42 mg QUE/g, than its biofractions. EEBF showed considerable antioxidant activity with IC50=19.04±0.24 μg/mL and IC50=65.85±1.22 μg/mL when compared to the standards Ascorbic acid (IC50=12.06±0.05 μg/mL) and Gallic acid (IC50=64.65±0.72 μg/mL) in DPPH and nitric oxide scavenging assays, respectively. MFBF showed significant activity with IC50=26.76±0.75 μg/mL in hydrogen peroxide scavenging assay compared to the standard Gallic acid (IC50=76.60±1.31 μg/mL). TFBF showed favourable growth inhibition of MCF-7 cells with GI50=73.5±11.96 µg/mL when compared to other samples screened (GI50>80 μg/mL) as against the standard Adriamycin (GI50<10 μg/mL) in SRB assay. The therapeutic virtues of EEBF and MFBF as free radical scavengers and TFBF as an antiproliferative may be attributed to the phenolics, flavonoids, steroids and triterpenoids present.

Keywords:

DPPH (2-2-diphenyl-1-picrylhydrazyl), Hydrogen Peroxide, MCF-7 Cells, Nitric Oxide, Sulforhodamine B

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References

Alam, M. N., Bristi, N. J., Rafiquzzaman, M. 2013. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal, 21(2):143–152.

Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D., Lightfoot, D. 2017. Phytochemicals: Extraction, Isolation, and Identification of Bioactive Compounds from Plant Extracts. Plants, 6(4):42.

Bandyopadhyay, S., Thothathri, K., Sharma, B. D. 2005. The genus Bauhinia L. (Leguminosae: Caesalpinioideae) in India. Journal of Economic and Taxonomic Botany, 29(4):763–801.

Boora, F., Chirisa, E., Mukanganyama, S. 2014. Evaluation of Nitrite Radical Scavenging Properties of Selected Zimbabwean Plant Extracts and Their Phytoconstituents. Journal of Food Processing, 2014:1–7.

Bouayed, J., Bohn, T. 2010. Exogenous Antioxidants—Double-Edged Swords in Cellular Redox State: Health Beneficial Effects at Physiologic Doses versus Deleterious Effects at High Doses. Oxidative Medicine and Cellular Longevity, 3(4):228–237.

Brighente, I. M. C., Dias, M., Verdi, L. G., Pizzolatti, M. G. 2007. Antioxidant Activity and Total Phenolic Content of Some Brazilian Species. Pharmaceutical Biology, 45(2):156–161.

Chanda, S., Dave, R. 2009. In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: An overview. African Journal of Microbiology Research, 3(13):981–996.

Chudzik, M., Korzonek-Szlacheta, I., Król, W. 2015. Triterpenes as Potentially Cytotoxic Compounds. Molecules, 20:1610–1625.

De Oliveira, A. M. F., Pinheiro, L. S., Pereira, C. K. S., Matias, W., Gomes, R., Chaves, O. S., Vanderlei De Souza, M. D. F., De Almeida, R. N., Simões de Assis, T. 2012. Total phenolic content and antioxidant activity of some Malvaceae family species. Antioxidants, 1(1):33–43.

Do, Q. D., Angkawijaya, A. E., Tran-Nguyen, P. L., Huynh, L. H., Soetaredjo, F. E., Ismadji, S., Ju, Y.-H. 2014. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis, 22(3):296–302.

Filho, V. C. 2009. Chemical composition and biological potential of plants from the genus Bauhinia. Phytotherapy Research, 23(10):1347–1354.

Gamit, S. B., Sapra, P., Vasava, M. S., Solanki, H. A., Patel, H., Rajani, D. 2018. Antimicrobial and antimalarial activities of some selected ethno-medicinal plants used by tribal communities of Tapi District, Gujarat, India. International Research Journal of Pharmacy, 9(10):151–156.

Gupta, A., Kumar, B. S., Negi, A. S. 2013. Current status on development of steroids as anticancer agents. The Journal of Steroid Biochemistry and Molecular Biology, 137:242–270.

Habbu, P. V., Miskin, N., Kulkarni, V. H., Bhat, P., Joshi, A., Bhandarkar, A., Kulkarni, S., Dixit, S. R., Vaijanathappa, J. 2020. Isolation, characterization, and cytotoxic studies of secondary metabolites from the leaves of Bauhinia foveolata Dalzell: An endemic tree from the Western Ghats, India. Journal of Applied Pharmaceutical Science, 10(03):135–148.

Kasote, D. M., Katyare, S. S., Hegde, M. V., Bae, H. 2015. Significance of Antioxidant Potential of Plants and its Relevance to Therapeutic Applications. International Journal of Biological Sciences, 11(8):982–991.

Khandelwal, K. R. 2010. Practical Pharmacognosy. Techniques and Experiments. 20th Edn. Nirali Prakashan.

Lalhminghlui, K., Jagetia, G. C. 2018. Evaluation of the free-radical scavenging and antioxidant activities of Chilauni, Schima wallichii Korth in vitro. Future Science OA, 4(2):FSO272.

Lopez, D., Cherigo, L., de Sedas, A., Spadafora, C., Martinez-Luis, S. 2018. Evaluation of antiparasitic, anticancer, antimicrobial and hypoglycemic properties of organic extracts from Panamanian mangrove plants. Asian Pacific Journal of Tropical Medicine, 11(1):32.

Malvia, S., Bagadi, S. A., Dubey, U. S., Saxena, S. 2017. Epidemiology of breast cancer in Indian women. Asia-Pacific Journal of Clinical Oncology, 13(4):289–295.

Mates, J. M., Segura, J. A., Alonso, F. J., Marquez, J. 2011. Anticancer Antioxidant Regulatory Functions of Phytochemicals. Current Medicinal Chemistry, 18(15):2315–2338.

Mathur, P., Sathishkumar, K., Chaturvedi, M., Das, P., Sudarshan, K. L., Santhappan, S., Nallasamy, V., John, A., Narasimhan, S., Roselind, F. S. 2020. Cancer Statistics, 2020: Report from National Cancer Registry Programme, India. JCO Global Oncology, 6(6):1063–1075.

Meda, A., Lamien, C. E., Romito, M., Millogo, J., Nacoulma, O. G. 2005. Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chemistry, 91(3):571–577.

Medeiros, B., Allan, A. L. 2019. Molecular Mechanisms of Breast Cancer Metastasis to the Lung: Clinical and Experimental Perspectives. International Journal of Molecular Sciences, 20(9):2272.

Medini, F., Fellah, H., Ksouri, R., Abdelly, C. 2014. Total phenolic, flavonoid and tannin contents and antioxidant and antimicrobial activities of organic extracts of shoots of the plant Limonium delicatulum. Journal of Taibah University for Science, 8(3):216–224.

Mishra, A., Sharma, A. K., Kumar, S., Saxena, A. K., Pandey, A. K. 2013. Bauhinia variegate Leaf Extracts Exhibit Considerable Antibacterial, Antioxidant, and Anticancer Activities. BioMed Research International, 2013:1–10.

Mukhopadhyay, D., Dasgupta, P., Roy, D. S., Palchoudhuri, S., Chatterjee, I., Ali, S., Dastidar, S. G. 2016. A Sensitive In vitro Spectrophotometric Hydrogen Peroxide Scavenging Assay using 1,10-Phenanthroline. Free Radicals and Antioxidants, 6:124–132.

Nely, M. M., Jenny, P. C., Yanet, C. O., Ruben, D. S., Carla, L. D., Luis, A. F. 2020. Evaluation of antioxidant potential and total phenolic content of exotic fruits grown in Colombia. Journal of Applied Pharmaceutical Science, 10(09):50–58.

Parul, R., Kundu, S. K., Saha, P. 2013. In vitro nitric oxide scavenging activity of methanol extracts of three Bangladeshi medicinal plants. The Pharma Innovation-Journal, 1(12):83–88.

Patel, D. K., Kumar, R., Prasad, S. K., Hemalatha, S. 2011. Pedalium murex Linn (Pedaliaceae) fruits: a comparative antioxidant activity of its different fractions. Asian Pacific Journal of Tropical Biomedicine, 1(5):395–400.

Pham-Huy, L. A., He, H., Pham-Huy, C. 2008. Free radicals, antioxidants in disease and health. International Journal of Biomedical Science, 4(2):3614697.

Skehan, P., Storeng, R., Scudiero, D., Monks, A., McMahon, J., Vistica, D., Warren, J. T., Bokesch, H., Kenney, S., Boyd, M. R. 1990. New Colorimetric Cytotoxicity Assay for Anticancer-Drug Screening. JNCI Journal of the National Cancer Institute, 82(13):1107–1112.

Vanicha Vichai, Kirtikara, K. 2006. Sulforhodamine B colorimetric assay for cytotoxicity screening. Nature Protocols, 1(3):1112–1116.

Vijayan, R., Joseph, S., Mathew, B. 2019. Anticancer, antimicrobial, antioxidant, and catalytic activities of green-synthesized silver and gold nanoparticles using Bauhinia purpurea leaf extract. Bioprocess and Biosystems Engineering, 42(2):305–319.

Zakaria, Z. A., Rofiee, M. S., Teh, L. K., Salleh, M. Z., Sulaiman, M. R., Somchit, M. N. 2011. Bauhinia purpurea leaves’ extracts exhibited in vitro antiproliferative and antioxidant activities. African Journal of Biotechnology, 10(1):65–74.

Published

2021-07-07

How to Cite

Liesl Maria Fernandes e Mendonça, Arun B Joshi, Anant V Bhandarkar, & Himanshu Joshi. (2021). Evaluation of antioxidant property and anticancer prospective of the leaf extract and biofractions of Bauhinia foveolata Dalzell – A native of the Indian Western Ghats. International Journal of Research in Pharmaceutical Sciences, 12(3), 1886–1894. Retrieved from https://ijrps.com/index.php/home/article/view/124

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