Detection of metallo beta lactamase genes in heteroresistant Pseudomonas aeruginosa isolated from clinical isolates in Egypt

Alaa El-Din M.S. Hosny (1) , Ali A. Abdelrahman (2) , Dalia M. Hamed (3) , Samira Zakeer (4)
(1) Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt, Egypt ,
(2) Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt, Egypt ,
(3) Department of Quality Control, VACSERA, Giza, Egypt, Egypt ,
(4) Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt, Egypt

Abstract

The aim of the study is to isolate and characterize Pseudomonas aeruginosa recovered from different clinical specimens and then study the susceptibility of the isolated strains to different antibiotics, screening for heteroresistant isolates and detecting the metalloβ-lactamase genes in these isolates. A total of two hundred and fifty clinical isolates were collected from which one hundred forty five isolates revealed Pseudomonas aeruginosa. They were collected from different clinical specimens applied for bacteriological testing from hospitalized in-patients admitted to Kasr Al Aini Hospital and Al-Demerdash Hospital, Egypt, in the period from February 2016 to December 2017. Antibiotic susceptibility testing was done using ten antibiotics. The study covered the heteroresistance of P.aeruginosa  towards several classes of antibiotics to make the statistical analysis convenient and to overview the significance of this resistance. The minimum inhibitory concentration was detected for the heteroresistant P.aeruginosa  resistant isolates.  The polymerase chain reaction of the heteroresistant strains was performed to detect the metallo-β-lactamase genes SIM, IMP and SPM and then sequencing was done consequently. SIM, IMP and SPM metalloβ-lactam e genes were detected in the heteroresistant isolates. The isolates showed a high resistance pattern to ampicillin (98.62%) and a high sensitivity rate to imipenem (96.55%) and the IMP gene was the highly significant gene.

Full text article

Generated from XML file

References

Alam, M. R., Donabedian, S., Brown, W., Gordon, J., Chow, J. W., Zervos, M. J., Hershberger, E. 2001. Heteroresistance to Vancomycin in Enterococcus faecium. Journal of Clinical Microbiology, 39(9):3379–3381. ISSN: 0095-1137.

Ayalew, K., Nambiar, S., Yasinskaya, Y., Jantausch, B. A. 2003. Carbapenems in Pediatrics. Therapeutic Drug Monitoring, 25:593–599. ISSN: 0163- 4356.

Balasubramanian, D., Harper, L., Shopsin, B., Torres, V. J. 2017. Staphylococcus aureus pathogenesis in diverse host environments. Pathogens and Disease, 75(1): ftx005. ISSN: 2049-632X.

Baldwin, C. M., Lyseng-Williamson, K. A., Keam, S. J. 2008. Meropenem a review of its use in the treatment of serious bacterial infections. Drugs, 68(6):803–841.

Bush, K., Jacoby, G. A., Medeiros, A. A. 1995. A functional classification scheme for beta lactamases and its correlation with molecular structure. Antimicrobial Agents and Chemotherapy, 39(6):1211–1233. ISSN: 0066-4804, 1098-6596.

Campanile, F., Borbone, S., Perez, M., Bongiorno, D., Cafiso, V., Bertuccio, T., Purrello, S., Nicolosi, D., Scuderi, C., Stefani, S. 2010. Heteroresistance to glycopeptides in Italian methicillin resistant Staphylococcus aureus (MRSA) isolates. International Journal of Antimicrobial Agents, 36(5):415– 419. ISSN: 0924-8579.

Chabbert, Y. A. 1967. Behaviour of methicillin heteroresistant Staphylococci to cephaloridine. Postgraduate Medical Journal, 43:40–42.

Cheesbrough, M. 2006. District Laboratory Practice in Tropical Countries, Part 2. volume 2, pages 9780511543470–9780511543470. Edition: 2nd, ISBN: 9780511543470.

Davies, J. C., Bilton, D. 2009. Bugs, biofilms, and resistance in cystic fibrosis. Respiratory care, 54(5):628–640.

Diab, M., Fam, N., El-Said, M., El-Defrawy, E. E. D. I., Saber, M. 2013. The occurrence of VIM-2 Metallo Lactamases in imipenem resistant and susceptible Pseudomonas aeruginosa clinical isolates from Egypt. African Journal of Microbiology Research, 7(35):4465–4472.

Dinesh, S. D., Grundmann, H., Pitt, T. L., Römling, U. 2003. European-wide distribution of Pseudomonas aeruginosa clone C. Clinical Microbiology and Infection, 9(12):1228–1233. ISSN: 1198-743X.

Drancourt, M., Bollet, C., Carlioz, A., Martelin, R., Gayral, J.-P., Raoult, D. 2000. 16S Ribosomal DNA Sequence Analysis of a Large Collection of Environmental and Clinical Unidentifiable Bacterial Isolates. Journal of Clinical Microbiology, 38(10):3623–3630. ISSN: 0095-1137, 1098-660X.

Fabiane, S. M., Sohi, M. K., Wan, T., Payne, D. J., Bate- son, J. H., Mitchell, T., Sutton, B. J. 1998. Crystal Structure of the Zinc-Dependent β-Lactamase from Bacillus cereusat 1.9 Å Resolution: Binuclear Active Site with Features of a Mononuclear Enzyme. Biochemistry, 37(36):12404–12411. ISSN: 0006-2960, 1520-4995.

Gales, A. C., Jones, R. N., Turnidge, J., Rennie, R., Ramphal, R. 2001. Characterization of Pseudomonas aeruginosaIsolates: Occurrence Rates, Antimicrobial Susceptibility Patterns, and Molecular Typing in the Global Sentry Antimicrobial Surveillance Program, 1997–1999. Clinical Infectious Diseases, 32(s2): S146–S155. ISSN: 1058-4838, 1537-6591.

Gordon, N. C., Wareham, D. W. 2009. Failure of the Micro Scan Walkaway System to Detect Heteroresistance to Carbapenems in a Patient with Enterobacter aerogenes Bacteremia. Journal of Clinical Microbiology, 47(9):3024–3025. ISSN: 0095-1137.

Hauser, A. R., Sriram, P. 2005. Severe Pseudomonas aeruginosa infections: tackling the conundrum of drug resistance. Postgraduate medicine, 117(1):41–48.

Huang, H., Siehnel, R. J., Bellido, F., Rawling, E., Hancock, R. E. 1992. Analysis of two gene regions involved in the expression of the imipenem-specific, outer membrane porin protein OprD of Pseudomonas aeruginosa. FEMS Microbiology Letters, 97(3):267–273. ISSN: 0378-1097, 1574-6968.

Ikonomidis, A., Neou, E., Gogou, V., Vrioni, G., Tsakris, A., Pournaras, S. 2009. Heteroresistance to Meropenem in Carbapenem-Susceptible Acinetobacter baumannii. Journal of Clinical Microbiology, 47(12):4055–4059. ISSN: 0095-1137.

Köhler, T., Kok, M., Michea-Hamzehpour, M., Plesiat, P., Gotoh, N., Nishino, T., Curty, L. K., Pechere, J. C. 1996. Multidrug efflux in intrinsic resistance to trimethoprim and sulfamethoxazole in Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy, 40(10):2288–2290. ISSN: 0066- 4804, 1098-6596.

Lister, P. D., Wolter, D. J., Hanson, N. D. 2009. Antibacterial-Resistant Pseudomonas aeruginosa: Clinical Impact and Complex Regulation of Chromosomally Encoded Resistance Mechanisms. Clinical Microbiology Reviews, 22(4):582–610. ISSN: 0893-8512, 1098-6618.

Majiduddin, F. K., Materon, I. C., Palzkill, T. G. 2002. Molecular analysis of beta-lactamase structure and function. International Journal of Medical Microbiology, 292(2):127–137. ISSN: 1438-4221.

Mansour, S. A., Eldaly, O., Fatani, A. J., Mohamed, M. L., Ibrahim, E. M. 2013. Epidemiological characterization of P. aeruginosa isolates of intensive care units in Egypt and Saudi Arabia. Eastern Mediterranean Health Journal, 19(01):71–80. ISSN 1020-3397, 1687-1634.

Paterson, D. L., Bonomo, R. A. 2005. Extended-Spectrum β-Lactamases: A Clinical Update. Clinical Microbiology Reviews, 18(4):657–686. ISSN: 0893-8512, 1098-6618.

Pirnay, J.-P., De Vos, D., Mossialos, D., Vanderkelen, A., Cornelis, P., Zizi, M. 2002. Analysis of the Pseudomonas aeruginosa oprD gene from clinical and environmental isolates. Environmental Microbiology, 4(12):872–882. ISSN: 1462-2912, 1462- 2920.

Pitout, J. D. D., Revathi, G., Chow, B. L., Kabera, B., Kariuki, S., Nordmann, P., Poirel, L. 2008. Metallo-β-lactamase-producing Pseudomonas aeruginosa isolated from a large tertiary centre in Kenya. Clinical Microbiology and Infection, 14(8):755–759. ISSN: 1198-743X.

Pournaras, S., Ikonomidis, A., Markogiannakis, A., Maniatis, A. N., Tsakris, A. 2005. Heteroresistance to carbapenems in Acinetobacter baumannii. Journal of Antimicrobial Chemotherapy, 55(6):1055– 1056. ISSN: 0305-7453, 1460-2091.

Schelin, J., Wallin-Carlquist, N., Cohn, M. T., Lindqvist, R., Barker, G. C. 2011. The formation of Staphylococcus aureusenterotoxin in food environments and advances in risk assessment. Virulence, 2(6):580–592. ISSN: 2150-5594, 2150-5608.

Shah, D., Narang, M. 2005. Meropenem. Indian Pediatric Journal, 42:443–50.

Tam, V. H., Schilling, A. N., Neshat, S., Poole, K., Melnick, D. A., Coyle, E. A. 2005. Optimization of Meropenem Minimum Concentration/MIC Ratio to Suppress In Vitro Resistance of Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy, 49(12):4920–4927. ISSN: 0066-4804, 1098-6596.

Valladares, M. H., Felici, A., Weber, G., Adolph, H. W., Zeppezauer, M., Rossolini, G. M., Galleni, M. 1997. Zn (II) dependence of the Aeromonas hydrophila AE036 metallo-β-lactamase activity and stability. Biochemistry, 36(38):11534–11541.

Walsh, T. R., Li, H., Toleman, M. A., Bennett, P. M., Jones, R. N. 2008. 179-base-pair plasmid harboring the blaVIM-7 Metallo-β-Lactamase gene in a Pseudomonas aeruginosa isolate from the United States. Antimicrobial Agents of Chemotherapy, 24(9):3099–105.

Yau, W., Owen, R. J., Poudyal, A., Bell, J. M., Turnidge, J. D., Yu, H. H., Nation, R. L., Li, J. 2009. Colistin hetero-resistance in multidrug-resistant Acinetobacter baumannii clinical isolates from the Western Pacific region in the SENTRY antimicrobial surveillance programme. Journal of Infection, 58(2):138–144. ISSN: 0163-4453.

Yetkin, G., Otlu, B., Cicek, A., Kuzucu, C., Durmaz, R. 2006. Clinical, microbiologic, and epidemiologic characteristics of Pseudomonas aeruginosa infections in a University Hospital, Malatya, Turkey. American Journal of Infection Control, 34(4):188– 192. ISSN: 0196-6553.

Authors

Alaa El-Din M.S. Hosny
Ali A. Abdelrahman
Dalia M. Hamed
daliamohsen80@gmail.com (Primary Contact)
Samira Zakeer
Alaa El-Din M.S. Hosny, Ali A. Abdelrahman, Dalia M. Hamed, & Samira Zakeer. (2021). Detection of metallo beta lactamase genes in heteroresistant Pseudomonas aeruginosa isolated from clinical isolates in Egypt. International Journal of Research in Pharmaceutical Sciences, 12(2), 1127–1135. Retrieved from https://ijrps.com/home/article/view/102

Article Details

No Related Submission Found