Geno protective efficacy of D-Pinitol isolated from aerial parts of Glycine max L. (Merr.) on Doxorubicin-mediated genotoxicity assessed through in vivo comet assay

Abstract

The goal of this investigation was to determine whether D-pinitol could protect against doxorubicin-mediated genotoxicity using the in vivo Comet test. Ten groups of Swiss albino mice (n = 6) were formed. Group I (Control) received a 0.9% NaCl treatment for fifteen days. Group II (Positive control) received doxorubicin (DOX) at 5 mg/kg intraperitoneally (i.p.) for three days on the first, eighth, and fifteenth days. Mice were administered D-pinitol (D-P) at various concentrations (100 mg/kg, 200 mg/kg, 300 mg/kg, and 400 mg/kg p.o.) for 15 days, categorized as Groups III, IV, V, and VI based on increasing D-P dosages. Group VII received DOX followed by D-P 100 mg/kg, while Group VIII received D-P 200 mg/kg before DOX. Group IX received D-P 300 mg/kg before DOX, and Group X received D-P 400 mg/kg before DOX. The in vivo Comet assay assessed DNA damage after collecting EDTA blood samples (50 µl) from all experimental and control groups. A highly significant increase in DNA damage (P<0.001) was observed in the DOX-only group. D-P showed no significant effect on DNA damage compared to the Control group. However, the group receiving D-P before DOX demonstrated a significant (P<0.001) dose-dependent reduction in DNA damage compared to the positive control.