Evaluation of the total antioxidant capacity, total polyphenol content, and flavonoids content in ethanolic fruit extract of Raphia hookeri and determination of the correlation between the antioxidant effects and the phytochemical contents

Akighir  J; Yakubu  O E; Imo  C; Ojogbane  E; Owuna  R I; Jato  J A

doi:10.26452/ijrps.v14i3.4306

Akighir J ⁽¹⁾ , Yakubu O E ⁽²⁾ , Imo C ⁽³⁾ , Ojogbane E ⁽⁴⁾ , Owuna R I ⁽⁵⁾ , Jato J A ⁽⁶⁾

(1) Department of Biochemistry, Federal University Wukari 200 Katsina-Ala Road, P.M.B 1020 Wukari, Taraba State, Nigeria, Nigeria ,

(2) Department of Biochemistry, Federal University Wukari 200 Katsina-Ala Road, P.M.B 1020 Wukari, Taraba State, Nigeria, Nigeria ,

(3) Department of Biochemistry, Federal University Wukari 200 Katsina-Ala Road, P.M.B 1020 Wukari, Taraba State, Nigeria, Nigeria ,

(4) Department of Biochemistry, Taraba State University P.M.B. 1167 Jalingo, Taraba State, Nigeria, Nigeria ,

(5) Department of Medical Laboratory Science, Hospitals Management Board, Makurdi-Aliade Road, Beside Road Safety Office P.M.B 102338 Makurdi, Benue State Nigeria, Nigeria ,

(6) Department of Biochemistry, Joseph SaawuanTarka University P.M.B. 2373 Makurdi, Benue State, Nigeria, Nigeria

https://doi.org/10.26452/ijrps.v14i3.4306

Issue
Vol. 14 No. 3 (2023): Volume 14 Issue 3

Keywords:

Raphia hookeri, total antioxidant capacity, total polyphenol content, total flavonoids content, Thiobarbituric Acid Reactive Substance, Superoxide dismutase and catalase

PDF LaTeX HTML ePUB

Abstract

In order to assess the total antioxidant capacity (TAC), total polyphenol content (TPC), and total flavonoid content (TFC) in ethanol fruit extract, as well as to ascertain the relationship between the antioxidant effects and phytochemical levels, this study looked at all three variables. 11 groups of 10 rats each were formed out of the experimental animals. All experimental subjects received a single intraperitoneal injection of aluminium chloride, 4.2 mg/kg body weight. Groups 4 and 5 received n-hexane fraction at 10 and 20 mg/kg b. w., respectively, whereas the other groups received various fractions and crude extracts of R. hookeri. Ethyl acetate fraction was given to groups 6 and 7 at doses of 10 and 20 mg/kg b. w. Aqueous fractions at 10 and 20 mg/kg b. w. were given to groups 8 and 9. While groups 10 and 11 got ethanolic crude extract daily for 21 days at doses of 200 mg/kg b. w. and 400 mg/kg b. w., respectively. All groups that were given AlCl3 - induced TBARS showed a significant (P 0.05) increase in vivo antioxidant activity. However, in the liver, kidney, and testes of all treatment groups, superoxide dismutase (SOD) and catalase (CAT) activity considerably increased (P 0.05). This suggests that a significant portion of the antioxidant activity displayed by the ethanolic fruit extract of R. hookeri is due to flavonoids.

Full text article

Generated from XML file

References

P Si-Yuan, L G4erhard, G Si-Hua, Z Shu- Feng, Y Zhi-Ling, Chen Hou-Q, Z Shuo-Feng, T Min-Ke, Sun Jian-Ning, and K Kam-Ming. Historical Perspective of Traditional Indigenous Medical Practices: The Current Renaissance and Conservation of Herbal Resources. Evidence-Based Complementary and Alternative Medicine, 2014, 2014.

M R Ahsan, K M Islam, M E Haque, and M A Mossaddik. In vitro antibacterial screening and toxicity study of some different medicinal plants. World Journal of Agriculture Science, 5(5):617–621, 2009.

A Matkowski, M Hajones, K Skalicka-Wozniak, and K Slusarczy. Antioxidant activity of polyphenols from Lycopus lucidus Turcz. Food Chemistry, 120(4):88–89, 2009.

A P Oluyori, D Oluwasogo, and Adejumoke. Phytochemical Analysis and Antioxidant Potential of Raphia Hookeri Leaf and Epicarp. Oriental Journal of Chemistry, (6):34–34, 2018.

Shaik Kasmoor Kalesha Vali, Narendra Kumar Reddy Kolli, and P. Swetha. Study of antihypertensive agents in rural areas by the community pharmacists. Indian Journal of Research in Pharmacy and Biotechnology, 5(1):28–30, 2017.

E J Akpan and F I Usoh. Phytochemical screening and effect of aqueous root extract of Raphia hookeri (raffia palm) on metabolic clearance rate of ethanol in rabbits. Nigerian Society for Experimental Biology, 16(1):37–42, 2004.

A Bauer and M Brönstrup. Industrial natural product chemistry for drug discovery and development. pages 35–60, 2014.

D J Newman and G M Cragg. Natural Products as Sources of New Drugs over the Nearly Four Decades from 01/1981 to 09/2019. Journal of Natural Products, 83(3):770–803, 2020.

G H Schmelzer and E A Omino. Proceedings of the first PROTA international Workshop. pages 23–25, 2002.

A Adedotun, I B Temitope, and G O Afolan Yan. Sublethal Effects of methanolic Extracts of R. hookeri on the reproductive capacity of Clarias gariepinus. Advances in Zoology, 2014.

N Ncube, S A J Afolayan, and A I Okoh. Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends. African Journal for Biotechnol, 7(12):1797–1806, 2008.

V T Nguyen, J A Sakoff, and C J Scarlett. Physicochemical properties, antioxidant and cytotoxic activities of crude extracts and fractions from Phyllanthus amarus. Medicines, pages 42–42, 2017.

E Yakubu, O F C Nwodo, P E Joshua, M N Ugwu, A D Odu, and F Okwo. Fraction and determination of total antioxidant capacity, total phenolic and total flavanoid contents of aqueous, ethanol and n-hexane extracts of vitex doniana leaves. African Journal for Biotechnology, 13(5):693–698, 2014.

Coskun. Separation techniques: Chromatography. Northern Clinics of Istanbul, 3:156–160, 2016.

V L Singleton, R Orthofer, and R M Lamuela-Raventos. Analysis of total phenol and other oxidative substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymology, 299:152–169, 2002.

J Lachman, K Hamouz, M Orsak, and V Pivec. Potato tubers as a significant source of antioxidants on human nutrition. Rostl. Vyr, 46:231– 236, 2000.

C C Chang, M H Yang, H M Wen, and J C Chm. Estimation of total flavonoids in propolis by two complementary colorimetric methods. Journal of Food Drug Annals, 10:178–182, 2002.

E Thomas, T P Aneesh, D G Thomas, and R Ananda. GC-MS analysis of phytochemical compounds present in rhizomes of Nervilia aragoana gaud. 2013.

A D Jesus and R B Chad. Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay. pages 12–12, 2020.

J W Christine and J C Joseph. Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue. Natural protocols, 5:51–66, 2010.

L A Goth. Simple method for determination of serum catalase activity and revision of reference range. Clinica Chimica Acta, 196:143–152, 1991.

Authors

Akighir J

Department of Biochemistry, Federal University Wukari 200 Katsina-Ala Road, P.M.B 1020 Wukari, Taraba State, Nigeria

johnakighir2016@gmail.com (Primary Contact)

Yakubu O E

Department of Biochemistry, Federal University Wukari 200 Katsina-Ala Road, P.M.B 1020 Wukari, Taraba State, Nigeria

Imo C

Department of Biochemistry, Federal University Wukari 200 Katsina-Ala Road, P.M.B 1020 Wukari, Taraba State, Nigeria

Ojogbane E

Department of Biochemistry, Taraba State University P.M.B. 1167 Jalingo, Taraba State, Nigeria

Owuna R I

Department of Medical Laboratory Science, Hospitals Management Board, Makurdi-Aliade Road, Beside Road Safety Office P.M.B 102338 Makurdi, Benue State Nigeria

Jato J A

Department of Biochemistry, Joseph SaawuanTarka University P.M.B. 2373 Makurdi, Benue State, Nigeria

J, A. ., O E, Y. ., C, I. ., E, O. ., R I, O. ., & J A, J. . (2023). Evaluation of the total antioxidant capacity, total polyphenol content, and flavonoids content in ethanolic fruit extract of Raphia hookeri and determination of the correlation between the antioxidant effects and the phytochemical contents. International Journal of Research in Pharmaceutical Sciences, 14(3), 141–152`. https://doi.org/10.26452/ijrps.v14i3.4306