Development and Validation of an HPTLC Method for Qualitative and Quantitative Estimation of Quercetin in Glinus oppositifolius (L.)
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Quercetin, HPTLC, Glinus oppositifolius (L.), Flavonoid
Abstract
Glinus oppositifolius (L.) is a perennial herb used in Indian folk medicine as a stomachic, aperients, antiseptic, uterine stimulant, and to promote menses and lochia. The reported pharmacological activities of this plant are immunomodulatory, hepatoprotectivite, anthelmintic, anti-hyperglycemic etc. activities. To an HPTLC densitometric method was developed and validated for the qualitative and quantitative estimation quercetin in Glinus oppositifolius (L.) available from West Bengal. The shad-dried leaves of Glinus oppositifolius (L.) were extracted with Methanol. HPTLC analysis was carried out on aluminum-backed silica gel 60 F254 plates with Ethyl acetate-Toluene–Formic acid 5:4:0.2 (v/v/v) as mobile phase. The HPTLC densitometric method was developed and validated as per ICH guidelines for estimation of quercetin. Total Flavonoid Content (TFC) is 102.95 ± 3.85 mg QE/ gm. In HPTLC analysis, G. oppositifolius ethanolic extract showed a maximum of 8 well-resolved peaks at Rf 0.005, 0.098, 0.266, 0.466, 0.655, 0.724, 0.776 and 0.827. Well separated and compact spots (Rf) of quercetin (0.81±0.06) were detected. The regression equation obtained was y = 0.0002x + 0.0019, with a correlation coefficient (R2) of 0.9852. The linearity range (µg/spot) 20-100. Quercetin content was found to be 0.25 ± 0.0047 mg of quercetin /100gm sample.The developed method was fond precise, robust an accurate and was successfully used for the detection and quantification of quercetin in Glinus oppositifolius (L.) and the quantities of quercetin was 0.25 ± 0.0047 mg of quercetin /100gm sample.
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