Development and validation of chemometric assisted analytical methods for simultaneous estimation of Atorvastatin calcium and Aspirin in capsule dosage form

Abstract

To develop two analytical methods like UV and RP-HPLC methods for the quantification of calcium (ASC) and Aspirin (APN) in the capsule dosage form. models used in UV were Principal component regression model (PCRM) and Partial least-square regression (PLSR). Both the models were applied for the drugs in the calibration ranges of 4-20 and 30-150 / for ASC and APN respectively. Total of nineteen laboratory prepared mixtures were used for calibration and prediction set of the models. In addition, RP-HPLC method by using approach for was developed using C18 column at room temperature with a mobile phase of : methanol: (53.1:11.9:35 v/v/v), pH- 3.0, with detection at 275 nm.PCRM and PLSR models were evaluated by statistical parameters and RP-HPLC method was optimized by using Response surface methodology. The developed methods like UV and RP-HPLC by using showed almost similar results and both the methods can be used for their analysis.