Cytokine Mediated Immunomodulatory Properties of Kaempferol-5-O-β-D- glucopyranoside from methanol extract of aerial parts of Indigofera aspalathoides Vahl ex DC

Abstract

The objective of the study was to evaluate the immunomodulatory effect of isolate compound Kaempferol-5-O-β- D-glucopyranoside from methanol extract of aerial parts of Indigofera aspalathoides Vahl ex DC. on Swiss albino rats by Cyclophosphamide induced myelosuppression, In vivo phagocytic activity by carbon clearance assay, Pla- que forming cell (PFC) assay, flow cytometric studies for the estimation of T lymphocytes (CD3 and CD19) and Th1 cytokines (IL-2, IFN-γ and IL-4). Levamisole (50 mg/kg b.w.p.o) and Cyclophosphamide (30mg/kg b.w.i.p.) were used as immunostimulatory and immunosuppressive standard drugs. Administration of Kaempferol-5-O-β-D- glucopyranoside at the doses of 10, 20, 40, 50 mg/kg b. w. p. o. showed significant increase in the phagocytic in- dex and in Plaque forming cell assay, and an significant protection against cyclophosphamide induced neutropenia and showed dose dependent increase in the production of T lymphocytes (CD3 and CD19) and Th 1 cytokines (IL-2, IFN-γ and IL-4). The maximum increase in the production of CD3, CD19, IL-2, IFN-γ and IL-4 at 50 mg kg b.w of the test compound was observed. The above results suggest that Kaempferol-5-O-β-D-glucopyranoside from methanol extract of aerial parts of Indigofera aspalathoides Vahl ex DC. can be further explored for the development of potent immunomodulatory drug.