Evaluation of in vitro antioxidant activity of ethanolic extract of leaves of Rhynchosia beddomei Baker

Abstract

To evaluate the antioxidant capability of the ethanolic extract of the leaves of Rhynchosia beddomei. In vitro antioxidant activity was evaluated by studying total phenol content, flavonoid content, DPPH radical scavenging activity, nitric oxide scavenging activity, hydrogen peroxide scavenging activity, reducing power, super ion scavenging activity and metal chelating activity. Gallic acid, ascorbic acid, Butylated hydroxyl toluene and quercetin were used as standards for all the experiments. The total phenolic content, total flavonoid content, and IC₅₀ values were observed for DPPH radical scavenging activity, nitric oxide scavenging activity, hydrogen peroxide scavenging activity, reducing power, super ion scavenging activity and metal chelating activities were determined to be 52.59, 48.6, 209, 191.18, 192.14, 218.4, 190.97 and 220.73. Each experiment was carried out in triplicate and results were expressed as mean ± SD. The results clearly indicated that ethanolic extract of leaves of Rhynchosia beddomei of the study species is effective in scavenging free radicals and has the potential to be a powerful in vitro antioxidant.