Antifungal activity of catecholate type siderophore produced by Bacillus sp.

Bharucha UD (1) , Patel KC (2) , Trivedi UB (3)
(1) M. B. Patel Science College, Anand – 388 001, Gujarat, India, India ,
(2) B.R.D. School of Biosciences, Sardar Patel University, Vallabh Vidyanagar – 388 120, Gujarat, India, India ,
(3) B.R.D. School of Biosciences, Sardar Patel University, Vallabh Vidyanagar – 388 120, Gujarat, India, India

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Vol. 4 No. 4 (2013): Volume 4 Issue 4
Keywords:
    Bacillus sp., siderophore, catecholate, bio-control
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Abstract

Siderophore production by Bacillus sp. isolated from rhizospheric soil of a medicinally and agriculturally important plant alfalfa (Medicago sativa L.) and its antifungal activity on two common plant pathogens (Fusarium oxysporum and Aspergillus niger) has been described in present work. Siderophores during their production in succinate medium were detected by the Chrome Azurol S (CAS) assay. Arnow’s assay was performed to reveal the catecholate type of siderophore. Extraction by ethyl acetate yielded 200 mg/L of siderophore. The purified siderophore was subjected to antifungal assay to check its bio-control potential and was found to inhibit the common plant pathogens Fusarium oxysporum and Aspergillus niger.

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Authors

Bharucha UD
M. B. Patel Science College, Anand – 388 001, Gujarat, India
Patel KC
B.R.D. School of Biosciences, Sardar Patel University, Vallabh Vidyanagar – 388 120, Gujarat, India
Trivedi UB
B.R.D. School of Biosciences, Sardar Patel University, Vallabh Vidyanagar – 388 120, Gujarat, India
ubt.spu@gmail.com (Primary Contact)
Bharucha UD, Patel KC, & Trivedi UB. (2013). Antifungal activity of catecholate type siderophore produced by Bacillus sp. International Journal of Research in Pharmaceutical Sciences, 4(4), 528–531. Retrieved from https://ijrps.com/home/article/view/3723
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