Method development and validation for the simultaneous determination of omeprazole in sold dosage form by RP-HPLC

Abstract

The present work describes a simple Reverse Phase HPLC method for the determination of Omeprazole from Capsule formulations. The determination was carried out on a Gasco, ODS, C-18 (250×4. 5mm, 5 microns) column using a mobile phase of Methanol: HPLC water (80:20). The flow rate and runtime were 1 ml/min and 10 min, respectively. The eluent was monitored at 280 nm. The method was reproducible, with good resolution and sharp peak of omeprazole. The detector response was found to be linear in the concentration range of 6-20 µg/ml for omeprazole.