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\begin{document}


\def\RunningAuthor{Pranaya P and Akila Devi D}
\firstPage{3475}
\articleType{Original Article}
\receivedDate{27.05.2019}
\acceptedDate{18.08.2019}
\revisedDate{13.08.2019}
\journalVolume{10}
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\journalDoi{ijrps.v10i4.1664}
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\title{Study of in-vitro antioxidant activity of various extracts of aerial parts of\textit{\space } \textit{Olax scandens} \textbf{\space }}
\author{Pranaya~P\textsuperscript{*}\textsuperscript{1}, 
            Akila~Devi~D\textsuperscript{2}~\\[5pt]{\textsuperscript{1}Sultan Ul Uloom College of Pharmacy, Hyderabad, India}~\\{\textsuperscript{2}School of Pharmaceutical Sciences, Vels University, Pallavaram, Chennai, Tamil Nadu, India}}

\begin{abstract}
\textit{Olax scandens}Roxb. (family \textit{Olacaceae) }Available in\textit{\space } throughout tropical India. The current study, aerial parts of different concentrates of \textit{Olax scandens}was evaluated for its \textit{in-vitro } antioxidant potential by Diphenylpicrylhydrazyl activity, superoxide scavenging activity, iron chelating activity taking ascorbate, quercetin \& Ethylenediamine tetraacetate as the standard correspondingly. An IC\ensuremath{_{50}} value was originated that methanolic concentrates of \textit{Olax scandens}is more efficient in Diphenylpicrylhydrazyl radical, superoxide radical activity, Iron chelating capacity compared EA \& PE concentrates. The methanolic concentrates of \textit{Olax scandens }\& ascorbate exhibited antioxidant potential possessing IC\ensuremath{_{50\ }}226\ensuremath{\mu}g/ml \& 66\ensuremath{\mu}g/ml (DPPH). 185\ensuremath{\mu}g/ml \& 60\ensuremath{\mu}g/ml (Superoxide) , 287\ensuremath{\mu}g/ml \& 65\ensuremath{\mu}g/ml (iron-chelating Activity) respectively. Invitro antioxidant studies obviously show methanolic concentrates of \textit{Olax scandens} \textit{\space }have better antioxidant activity. These results indicate that aerial parts of methanolic concentrates \textit{Olax scandens }could serve as a natural antioxidant, which may be useful in preventing free radical-induced diseases. 
\end{abstract}\def\keywordstitle{Keywords}

\begin{keywords}Olax scandens,\newline DPPH radical scavenging,\newline Superoxide radical,\newline Iron chelating activity
\end{keywords}
\twocolumn[ \maketitle {\printKwdAbsBox}]
\makeatletter{\raggedright\textsuperscript{*}Corresponding Author\par Name:\ Pranaya~P~\\ Phone:\ 9443171712~\\  Email:\ akottaimuthu@gmail.com
        \par}\vspace*{-11pt}\hrulefill\par{\fontsize{12}{14}\selectfont ISSN: 0975-7538}\par% 
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\section{Introduction}
A free radical is any species capable of freelance existence that contains a lot of odd unpaired electrons. Electron unpaired being single that is only in an orbital \unskip~\citep{601208:13998241}. The electron unpaired gives convinced characteristic property to the free radical, like as paramagnetism. The chemical reactivity of free radicals is usually more. They may be + charged, - charged, or neutral charge electrically \unskip~\citep{601208:13998249}. These RO having a major task in the pathogenesis of many oxidative stress associated disorders like carcinogenesis, cardiovascular diseases, arthritis, diabetics, \& neurological disorders \unskip~\citep{601208:13998240}. The generally used synthetic antioxidants at present are BHA, BHT, PG, \& TBHQ. However, these drugs are assumed accountable for liver harm and performing as carcinogens in lab animals \unskip~\citep{601208:13998235}. The investigate for new products with antioxidative potential \& a smaller number of adverse effects is an active domain of research. Thus, the extension and utilization of huge efficient antioxidants of the natural source was popular \unskip~\citep{601208:13998246}.

\textit{Olax scandens }Roxb. (family \textit{Olacaceae} ) is commonly known as ``Parrot Olax, Sprawling olax '' in English \& locally known as `Kurpodur' in Telugu. This plants fruits and leaves has been used for therapeutic \& food purpose. \textit{O. Scandens leaves were} used as constipation. \textit{Olax scandens}(Roxb.), is generally known as Badrul in Odiya, used for cooking \& different therapeutic purposes\unskip~\citep{601208:13998248}. \textit{Olax scandens} bark decoction is used treatment fever \& cough.\unskip~\citep{601208:13998250}. \textit{O. scandens }leaves were used for mouth ulcers.\unskip~\citep{601208:13998236}. \textit{O.scandens} boiled leaves were applied in the head for the treatment of headache.\unskip~\citep{601208:13998244}. Still, no literature are available on the antioxidant potency of \textit{Olax scandens}. Thus, the present study to assess the antioxidant activities of \textit{Olax scandens.\textit{}}
    
\section{Materials and Methods}
\textbf{Gathering \& Identification of Plant }

The aerial parts of \textit{O.scandens}(family \textit{Olacaceae}) were gathered from Medak, Telangana state, India. Plant identification was made from the Botanical investigation of India, Telangana regional center, Hyderabad, (BSI/DRC/2019-20/Tech/174). The \textit{O.scandens,} were desiccated under shadowy, segregate, crushed through the grinder \unskip~\citep{601208:13998247}.


\begin{table*}[!htbp]
\caption{\boldmath {DPPH radical activity of \textit{Olax scandens} PE extract} }
\label{tw-aa019700ef50}
\centering 
\begin{threeparttable}

\def\arraystretch{1.1}
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\begin{tabulary}{\linewidth}{p{\dimexpr.1358\linewidth-2\tabcolsep}p{\dimexpr.19119999999999997\linewidth-2\tabcolsep}p{\dimexpr.3711\linewidth-2\tabcolsep}p{\dimexpr.3019\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}{\multirow{2}{*}{ S.no}} & {\multirow{2}{*}{ Extract \mbox{}\protect\newline  (\ensuremath{\mu}g/ml)}} & \multicolumn{2}{p{\dimexpr(.673\linewidth-2\tabcolsep)}}{\cAlignHack \% of activity(\ensuremath{\pm}SEM)*}\\
\rowcolor{kwdboxcolor} &  &  PE concentrates  &  Ascorbate\\
\tblmidrule 
1 &
  100 &
  10.29\ensuremath{\pm}0.023 &
  54.19 \ensuremath{\pm}0.024\\
2 &
  200 &
  19.74\ensuremath{\pm}0.045 &
  59.24 \ensuremath{\pm}0.032\\
3 &
  400 &
  34.56\ensuremath{\pm}0.063 &
  65.32 \ensuremath{\pm}0.054\\
4 &
  800 &
  45.08\ensuremath{\pm}0.043 &
  72.82 \ensuremath{\pm}0.062\\
\multicolumn{2}{p{\dimexpr(.32699999999999996\linewidth-2\tabcolsep)}}{} &
  IC50  = 990 \ensuremath{\mu}g/ml &
  IC50  = 66 \ensuremath{\mu}g/ml\\
\tblbottomrule 
\end{tabulary}\par 
\begin{tablenotes}\footnotesize 
    
\item{*Every value was articulated as mean \ensuremath{\pm} SEM for 3 experimentation}
\end{tablenotes}
\end{threeparttable}

\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {DPPH radical activity of \textit{Olax scandens} EA extract} }
\label{tw-3eaaff095fee}
\centering 
\begin{threeparttable}

\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.1272\linewidth-2\tabcolsep}p{\dimexpr.231\linewidth-2\tabcolsep}p{\dimexpr.3433\linewidth-2\tabcolsep}p{\dimexpr.2985\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}{\multirow{2}{*}{ S.no}} & {\multirow{2}{*}{ Extract \mbox{}\protect\newline  (\ensuremath{\mu}g/ml)}} & \multicolumn{2}{p{\dimexpr(.6418\linewidth-2\tabcolsep)}}{\cAlignHack \% of activity(\ensuremath{\pm}SEM)*}\\
\rowcolor{kwdboxcolor} &  &  (EA concentrates)  &  (Ascorbate)\\
\tblmidrule 
1 &
  100 &
  28.33 \ensuremath{\pm} 0.024 &
  54.19 \ensuremath{\pm}0.024\\
2 &
  200 &
  39.75 \ensuremath{\pm} 0.034 &
  59.24 \ensuremath{\pm}0.032\\
3 &
  400 &
  47.12 \ensuremath{\pm} 0.023 &
  65.32 \ensuremath{\pm}0.054\\
4 &
  800 &
  59.12 \ensuremath{\pm} 0.015 &
  72.82 \ensuremath{\pm}0.062\\
\multicolumn{2}{p{\dimexpr(.3582\linewidth-2\tabcolsep)}}{} &
  IC50  = 504\ensuremath{\mu}g/ml &
  IC50  = 66 \ensuremath{\mu}g/ml\\
\tblbottomrule 
\end{tabulary}\par 
\begin{tablenotes}\footnotesize 
    
\item{* Every value was articulated as mean \ensuremath{\pm} SEM for 3 experimentation}
\end{tablenotes}
\end{threeparttable}

\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {DPPH radical activity of \textit{Olax scandens} metrhanolic extract} }
\label{tw-af103ae1205e}
\centering 
\begin{threeparttable}

\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.1375\linewidth-2\tabcolsep}p{\dimexpr.18080000000000002\linewidth-2\tabcolsep}p{\dimexpr.44730000000000004\linewidth-2\tabcolsep}p{\dimexpr.2344\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}{\multirow{2}{*}{ S.no}} & {\multirow{2}{*}{ Extract \mbox{}\protect\newline  (\ensuremath{\mu}g/ml)}} & \multicolumn{2}{p{\dimexpr(.6817\linewidth-2\tabcolsep)}}{\cAlignHack \% of activity(\ensuremath{\pm}SEM)*}\\
\rowcolor{kwdboxcolor} &  &  (Methanolic concentrates)  & Ascorbate\\
\tblmidrule 
1 &
  100 &
  40.23\ensuremath{\pm}0.022 &
  54.19 \ensuremath{\pm}0.024\\
2 &
  200 &
  49.34\ensuremath{\pm}0.043 &
  59.24 \ensuremath{\pm}0.032\\
3 &
  400 &
  57.55\ensuremath{\pm}0.048 &
  65.32 \ensuremath{\pm}0.054\\
4 &
  800 &
  66.24\ensuremath{\pm}0.023 &
  72.82 \ensuremath{\pm}0.062\\
\multicolumn{2}{p{\dimexpr(.31830000000000002\linewidth-2\tabcolsep)}}{} &
  IC50  = 226\ensuremath{\mu}g/ml &
  IC50  = 66\ensuremath{\mu}g/ml\\
\tblbottomrule 
\end{tabulary}\par 
\begin{tablenotes}\footnotesize 
    
\item{* Every value was articulated as mean \ensuremath{\pm} SEM for 3 experimentation}
\end{tablenotes}
\end{threeparttable}

\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {Activity of PE concentrates of \textit{Olax scandens} on Superoxide radical method} }
\label{tw-551f2933000d}
\centering 
\begin{threeparttable}

\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.11690000000000001\linewidth-2\tabcolsep}p{\dimexpr.17200000000000003\linewidth-2\tabcolsep}p{\dimexpr.3694\linewidth-2\tabcolsep}p{\dimexpr.3417\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}{\multirow{2}{*}{ S.no}} & {\multirow{2}{*}{ Extract \mbox{}\protect\newline (\ensuremath{\mu}g/ml)}} & \multicolumn{2}{p{\dimexpr(.7111\linewidth-2\tabcolsep)}}{\cAlignHack \% inhibition (\ensuremath{\pm}SEM)*}\\
\rowcolor{kwdboxcolor} &  &  (PE concentrates)  & (Quercetin) \\
\tblmidrule 
1 &
  125 &
  19.15 \ensuremath{\pm}0.012 &
  72.76 \ensuremath{\pm}0.012\\
2 &
  250 &
  27.09 \ensuremath{\pm}0.014 &
  90.29 \ensuremath{\pm}0.014\\
3 &
  500 &
  35.33 \ensuremath{\pm}0.022 &
  96.89 \ensuremath{\pm}0.010\\
4 &
  1000 &
  48.65 \ensuremath{\pm}0.026 &
  99.12 \ensuremath{\pm}0.018\\
\multicolumn{2}{p{\dimexpr(.28890000000000004\linewidth-2\tabcolsep)}}{} &
  IC50  = 1070 \ensuremath{\mu}g/ml &
  IC50  = 60 \ensuremath{\mu}g/ml\\
\tblbottomrule 
\end{tabulary}\par 
\begin{tablenotes}\footnotesize 
    
\item{* Every value was articulated as mean \ensuremath{\pm} SEM for 3 experimentation}
\end{tablenotes}
\end{threeparttable}

\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {Activity of EA concentrates of \textit{Olax scandens} on Superoxide radical method} }
\label{tw-7320a08481e1}
\centering 
\begin{threeparttable}

\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.1531\linewidth-2\tabcolsep}p{\dimexpr.15829999999999995\linewidth-2\tabcolsep}p{\dimexpr.4368\linewidth-2\tabcolsep}p{\dimexpr.2518\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}{\multirow{2}{*}{ S.no}} & {\multirow{2}{*}{ Extract \mbox{}\protect\newline (\ensuremath{\mu}g/ml)}} & \multicolumn{2}{p{\dimexpr(.6886\linewidth-2\tabcolsep)}}{\cAlignHack \% of inhibition (\ensuremath{\pm}SEM)*}\\
\rowcolor{kwdboxcolor} &  &  (Ethyl acetate concentrates)  &  (Quercetin) \\
\tblmidrule 
1 &
  125 &
  20.12 \ensuremath{\pm}0.014 &
  72.76 \ensuremath{\pm}0.012\\
2 &
  250 &
  44.49 \ensuremath{\pm}0.025 &
  90.29 \ensuremath{\pm}0.014\\
3 &
  500 &
  50.23 \ensuremath{\pm}0.033 &
  96.89 \ensuremath{\pm}0.010\\
4 &
  1000 &
  64.12 \ensuremath{\pm}0.024 &
  99.12 \ensuremath{\pm}0.018\\
\multicolumn{2}{p{\dimexpr(.31139999999999993\linewidth-2\tabcolsep)}}{} &
  IC50  = 495 \ensuremath{\mu}g/ml &
  IC50  = 60 \ensuremath{\mu}g/ml\\
\tblbottomrule 
\end{tabulary}\par 
\begin{tablenotes}\footnotesize 
    
\item{* Every value was articulated as mean \ensuremath{\pm} SEM for 3 experimentation}
\end{tablenotes}
\end{threeparttable}

\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {Activity of Methanolic concentrates \textit{Olax scandens }on Superoxide radical method} }
\label{tw-53bb1fb23dcd}
\centering 
\begin{threeparttable}

\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.1099\linewidth-2\tabcolsep}p{\dimexpr.16869999999999997\linewidth-2\tabcolsep}p{\dimexpr.4264000000000001\linewidth-2\tabcolsep}p{\dimexpr.295\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}{\multirow{2}{*}{ S.no}} & {\multirow{2}{*}{ Extract \mbox{}\protect\newline (\ensuremath{\mu}g/ml)}} & \multicolumn{2}{p{\dimexpr(.7214000000000001\linewidth-2\tabcolsep)}}{\cAlignHack \% inhibition (\ensuremath{\pm}SEM)*}\\
\rowcolor{kwdboxcolor} &  & Methanolic extract  & Quercetin \\
\tblmidrule 
1 &
  125 &
  35.18 \ensuremath{\pm}0.071 &
  72.76 \ensuremath{\pm}0.012\\
2 &
  250 &
  54.23 \ensuremath{\pm}0.024 &
  90.29 \ensuremath{\pm}0.014\\
3 &
  500 &
  62.34 \ensuremath{\pm}0.044 &
  96.89 \ensuremath{\pm}0.010\\
4 &
  1000 &
  70.28 \ensuremath{\pm}0.017 &
  99.12 \ensuremath{\pm}0.018\\
\multicolumn{2}{p{\dimexpr(.2786\linewidth-2\tabcolsep)}}{} &
  IC50  = 185 \ensuremath{\mu}g/ml &
  IC50  = 60 \ensuremath{\mu}g/ml\\
\tblbottomrule 
\end{tabulary}\par 
\begin{tablenotes}\footnotesize 
    
\item{* Every value was articulated as mean \ensuremath{\pm} SEM for 3 experimentation}
\end{tablenotes}
\end{threeparttable}

\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {Iron-binding potential of \textit{O.scandens} PE concentrates} }
\label{tw-b843b57257c7}
\centering 
\begin{threeparttable}

\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.122\linewidth-2\tabcolsep}p{\dimexpr.18599999999999998\linewidth-2\tabcolsep}p{\dimexpr.3035\linewidth-2\tabcolsep}p{\dimexpr.3885\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}{\multirow{2}{*}{ S.no}} & {\multirow{2}{*}{ Extract \mbox{}\protect\newline  (\ensuremath{\mu}g/ml)}} & \multicolumn{2}{p{\dimexpr(.692\linewidth-2\tabcolsep)}}{\cAlignHack \% inhibition (\ensuremath{\pm}SEM)*}\\
\rowcolor{kwdboxcolor} &  &  PE concentrates  & Ethylenediamine tetraacetate \\
\tblmidrule 
1 &
  125 &
  26.90 \ensuremath{\pm}0.012 &
  57.52 \ensuremath{\pm}0.014\\
2 &
  250 &
  34.56 \ensuremath{\pm}0.032 &
  64.76 \ensuremath{\pm}0.022\\
3 &
  500 &
  42.65 \ensuremath{\pm}0.010 &
  82.12 \ensuremath{\pm}0.045\\
4 &
  1000 &
  51.76 \ensuremath{\pm}0.016 &
  96.34 \ensuremath{\pm}0.034\\
\multicolumn{2}{p{\dimexpr(.30799999999999997\linewidth-2\tabcolsep)}}{} &
  IC50  = 980 \ensuremath{\mu}g/ml &
  IC50  = 65 \ensuremath{\mu}g/ml\\
\tblbottomrule 
\end{tabulary}\par 
\begin{tablenotes}\footnotesize 
    
\item{* Every value was articulated as mean \ensuremath{\pm} SEM for 3 experimentation}
\end{tablenotes}
\end{threeparttable}

\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {Iron-binding potential of \textit{O.scandens} of EA concentrates } }
\label{tw-e1af5575b628}
\centering 
\begin{threeparttable}

\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.1133\linewidth-2\tabcolsep}p{\dimexpr.16700000000000003\linewidth-2\tabcolsep}p{\dimexpr.29849999999999998\linewidth-2\tabcolsep}p{\dimexpr.4212\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}{\multirow{2}{*}{ S.no}} & {\multirow{2}{*}{ Extract \mbox{}\protect\newline  (\ensuremath{\mu}g/ml)}} & \multicolumn{2}{p{\dimexpr(.7197\linewidth-2\tabcolsep)}}{\cAlignHack \% inhibition (\ensuremath{\pm}SEM)*}\\
\rowcolor{kwdboxcolor} &  &  EA concentrates  & Ethylenediamine tetraacetate \\
\tblmidrule 
1 &
  125 &
  32.30 \ensuremath{\pm}0.024 &
  57.52 \ensuremath{\pm}0.014\\
2 &
  250 &
  43.38 \ensuremath{\pm}0.013 &
  64.76 \ensuremath{\pm}0.022\\
3 &
  500 &
  50.28 \ensuremath{\pm}0.023 &
  82.12 \ensuremath{\pm}0.045\\
4 &
  1000 &
  64.70 \ensuremath{\pm}0.026 &
  96.34 \ensuremath{\pm}0.034\\
\multicolumn{2}{p{\dimexpr(.2803\linewidth-2\tabcolsep)}}{} &
  IC\ensuremath{_{50\ }} = 495\ensuremath{\mu}g/ml &
  IC\ensuremath{_{50}}  = 65\ensuremath{\mu}g/ml\\
\tblbottomrule 
\end{tabulary}\par 
\begin{tablenotes}\footnotesize 
    
\item{* Every value was articulated as mean \ensuremath{\pm} SEM for 3 experimentation}
\end{tablenotes}
\end{threeparttable}

\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {Iron-binding potential of \textit{O.scandens} Methanolic concentrates} }
\label{tw-f6a8f3426795}
\centering 
\begin{threeparttable}

\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.1531\linewidth-2\tabcolsep}p{\dimexpr.167\linewidth-2\tabcolsep}p{\dimexpr.3555\linewidth-2\tabcolsep}p{\dimexpr.3244\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}{\multirow{2}{*}{S.no}} & {\multirow{2}{*}{Extract \mbox{}\protect\newline (\ensuremath{\mu}g/ml)}} & \multicolumn{2}{p{\dimexpr(.6799\linewidth-2\tabcolsep)}}{\cAlignHack \% inhibition(\ensuremath{\pm}SEM)*}\\
\rowcolor{kwdboxcolor} &  & Methanol concentrates  & EthyleneDiamine tetra acetate  \\
\tblmidrule 
1 &
  125 &
  36.22 \ensuremath{\pm}0.010 &
  57.52 \ensuremath{\pm}0.014\\
2 &
  250 &
  47.42 \ensuremath{\pm}0.014 &
  64.76 \ensuremath{\pm}0.022\\
3 &
  500 &
  60.56 \ensuremath{\pm}0.016 &
  82.12 \ensuremath{\pm}0.045\\
4 &
  1000 &
  68.89 \ensuremath{\pm}0.021 &
  96.34 \ensuremath{\pm}0.034\\
\multicolumn{2}{p{\dimexpr(.3201\linewidth-2\tabcolsep)}}{} &
  IC\ensuremath{_{50}}  = 287\ensuremath{\mu}g/ml &
  IC\ensuremath{_{50}}  = 65\ensuremath{\mu}g/ml\\
\tblbottomrule 
\end{tabulary}\par 
\begin{tablenotes}\footnotesize 
    
\item{* Every value was articulated as mean \ensuremath{\pm} SEM for 3 experimentation}
\end{tablenotes}
\end{threeparttable}

\end{table*}
\textbf{Preparation of Concentrates }

The pulverized materials were progressively concentrated with PE (40-60\ensuremath{^{0}}C) through hot constant percolation  method in Soxhlet equipment \unskip~\citep{601208:13998243} for twenty-four hours. At that moment, the marc was subjected to EA (76-78\ensuremath{^{0}}C) for 24 hrs \& then mark was subjected to methanol for 24 hrs. The concentrates were concentrated through the rotational evaporator and subjected to solidify drying in a lyophilizer till dry powder was acquired. \unskip~\citep{601208:13998234}.

\textbf{Assessment of Antioxidant potential through invitro methods}

The variety of concentrates of \textit{O.scandens }were used assessment of antioxidant\textit{\space }activity by \unskip~\citet{601208:13998245} method was adopted for Diphenyl picrylhydrazyl radical assay \& \unskip~\cite{601208:13998251} method described for Superoxide radical (O\ensuremath{_{2}}\ensuremath{^{-}}) assay and \unskip~\cite{601208:13998237}ss method was adopted to determine the Iron chelating activity. 
    
\section{Results and Discussion}
\textbf{DPPH scavenging activity}

The DPPH activity of PE concentrates of \textit{Olax scandens }appeared Table~\ref{tw-aa019700ef50}. The PE concentrates of \textit{Olax \textit{Scandens }exhibit}, a more DPPH activity of 45.08\% at 800 \ensuremath{\mu}g/ml \& ascorbate was recorded 72.82\% at 800 \ensuremath{\mu}g/ml. The IC\ensuremath{_{50}} of the PE concentrates of \textit{Olax scandens }\& ascorbic acid were recorded 90\ensuremath{\mu}g/ml \& 66\ensuremath{\mu}g/ml correspondingly.

DPPH activity of EA concentrates of \textit{Olax scandens }summarized in Table~\ref{tw-3eaaff095fee}. The EA concentrates of \textit{Olax \textit{Scandens }exhibit} more DPPH scavenging potential of 59.12\% at 800 \ensuremath{\mu}g/ml \& ascorbate was recorded 72.82\% at 800 \ensuremath{\mu}g/ml. The IC\ensuremath{_{50}} of the EA concentrates of \textit{Olax scandens }\& ascorbic acid were recorded 504\ensuremath{\mu}g/ml \& 66\ensuremath{\mu}g/m correspondingly. 

DPPH potential of methanolic concentrates of \textit{O.scandens }appeared in Table~\ref{tw-af103ae1205e}. The methanolic concentrates of \textit{O.scandens }having more\textit{\space } DPPH scavenging potential of 66.24\% at 800 \ensuremath{\mu}g/ml \& ascorbate was recorded 72.82\% at 800 \ensuremath{\mu}g/ml. The IC\ensuremath{_{50}} of the methanolic concentrates of \textit{O.scandens }\& ascorbic acid were recorded 226\ensuremath{\mu}g/ml \& 66\ensuremath{\mu}g/m correspondingly. 

The methanolic concentrates of \textit{Olax scandens }was recorded to more activity than PE \& EA concentrates. The IC\ensuremath{_{50}} of the methanolic concentrates of \textit{O.scandens}\& ascorbic acid were found to be 226\ensuremath{\mu}g/ml \& 66\ensuremath{\mu}g/ml correspondingly. 

\textbf{Superoxide activity}

Superoxide radical potential of PE concentrates of \textit{O.scandens }appeared Table~\ref{tw-551f2933000d}. The more Superoxide radical potential of PE concentrates \& standard at 1000 \ensuremath{\mu}g/ml was recorded at 48.65\% \& 98.01 \%. IC\ensuremath{_{50}} of PE concentrates \& standard was recorded as 1070\ensuremath{\mu}g/millilitre \& 60\ensuremath{\mu}g/millilitre correspondingly. 

Superoxide radical potential of EA concentrates of \textit{O.scandens }appeared in Table~\ref{tw-7320a08481e1}. The more SO scavenging potential of EA concentrates \& standard 1000 \ensuremath{\mu}g/ml was recorded 64.12\% \& 99.12\% correspondingly. EA concentrates \& Quercetin IC\ensuremath{_{50}} was recorded as 495\ensuremath{\mu}g/ml \& 60\ensuremath{\mu}g/ml correspondingly. 

Superoxide radical scavenging potential of methanolic concentrates of \textit{O.scandens }appeared in Table~\ref{tw-53bb1fb23dcd}. Superoxide radical scavenging potential was more in methanolic concentrates \& Quercetin (standard) at 1000 \ensuremath{\mu}g/ml was recorded 70.28\% \& 99.12\% . Methanolic concentrates \& standard IC\ensuremath{_{50}} was recorded as 185\ensuremath{\mu}g/ml \& 60\ensuremath{\mu}g/ml correspondingly.

IC\ensuremath{_{50}} values \& Superoxide radical potential revealed that methanol concentrates of \textit{Olax scandens }is better activity in scavenging superoxide radical when compared EA \& PE extracts.

\textbf{Iron chelating potential}

The iron complex potential of PE concentrates \textit{O.scandens }\& Ethylenediamine tetraacetate were appeared in Table~\ref{tw-b843b57257c7}. The more iron-binding potential of PE concentrates \& Ethylenediamine tetraacetate  1000 \ensuremath{\mu}g/ml were recorded, 51.76\% \& 96.34 \%. The IC\ensuremath{_{50}} of PE concentrates of  \textit{O.scandens}\& Ethylenediamine tetraacetate were found as 980\ensuremath{\mu}g/ml \& 65\ensuremath{\mu}g/ml correspondingly.

The iron complex potential of EA concentrates of \textit{Olax scandens }\& Ethylenediamine tetraacetate were presented in Table~\ref{tw-e1af5575b628}. The more iron-binding capacity of EA concentrates \& Ethylenediamine tetraacetate 1000 \ensuremath{\mu}g/ml was recorded at 64.70\% \& 96.34 \%.  The IC\ensuremath{_{50}} value of ethyl acetate concentrates of \textit{Olax scandens} \& Ethylenediamine tetraacetate were found 495\ensuremath{\mu}g/ml \& 65\ensuremath{\mu}g/ml correspondingly.

The iron complex potential of methanolic concentrates of \textit{Olax scandens }\& Ethylenediamine tetraacetate were presented in Table~\ref{tw-f6a8f3426795}. The more iron-binding potential of methanolic concentrates \& Ethylenediamine tetraacetate 1000 \ensuremath{\mu}g/ml were recorded, 68.89\% \& 96.34 \%. The IC\ensuremath{_{50}} value of methanol concentrates of \textit{Olax scandens }\& Ethylenediamine tetraacetate was recorded as 287\ensuremath{\mu}g/ml \& 65\ensuremath{\mu}g/ml correspondingly.

IC\ensuremath{_{50}} values \& iron binding potential revealed that methanol concentrates of \textit{Olax scandens }is huge activity in iron chelating potential when compared ethyl acetate \& petroleum ether concentrates. But when compared to the all the three concentrates, the methanol concentrates of the \textit{Olax scandens }showed the better result.

Assessment of antioxidant activity, so many \textit{in vitro } methods have been used a variety of concentrates of \textit{Olax scandens}. Diphenylpicrylhydrazyl is a stable N\ensuremath{_{2}}-centered free radical generally utilized for testing the antioxidant potential of herbal concentrates. When the stable Diphenylpicrylhydrazyl radical accepts an electron from the antioxidant compound, the violet colour of the Diphenylpicrylhydrazyl as reduced to yellow colored diphenylpicrylhydrazine radical which was measured colorimetrically. Substances which are able to perform this reaction can be considered as antioxidants \& therefore radical scavengers \unskip~\citep{601208:13998238}. The results of antioxidant activity by Diphenylpicrylhydrazyl radical activity, superoxide radical activity \& iron-chelating potential were expressed in terms of \% inhibition of generated free radicals respectively with respect to various concentrations. Among the three different plant concentrates tested, interestingly, in the DPPH radical activity of the methanolic of \textit{Olax \textit{Scandens }exhibited} more Diphenylpicrylhydrazyl radical potential comparable with that of ascorbic acid. 

Superoxides could be produced in huge amounts by various biological processes. It is known to be more injurious to cellular components as an originator of the most ROS, contributing to tissue damage \& many disorders \unskip~\citep{601208:13998242}. The methanolic concentrates of \textit{Olax scandens }exhibited higher ability in scavenging superoxide anion radical when compared to the strand quercetin. 

 The iron-chelating potential of all the concentrates was measured by Fe-ferrozine complex formation. Ferrozine-Fe complex is producing red-colored, which absorbs at 562nm \unskip~\citep{601208:13998252}. It was revealed that Ethylenediamine tetraacetate, which forms \ensuremath{\sigma  } bond with iron, are efficient as secondary antioxidants, for the basis that they decrease the redox potential, thereby stabilizing the oxidized form of the iron ion \unskip~\citep{601208:13998239}. Iron chelating potential of methanolic concentrates of \textit{Olax \textit{Scandens }exhibited} higher ability in scavenging compared to standard Ethylenediamine tetraacetate. 
    
\section{Conclusion}
The current trends, antioxidative activity of the herbs having more interest due to their possible use as natural additives to substitute synthetic ones. Among the three various concentratess methanolic concentrates of \textit{Olax scandens }exhibited higher potency of antioxidant activity. These results indicate that aerial parts of methanolic concentrates \textit{Olax \textit{Scandens }could} serve as a natural antioxidant, which may be useful in preventing free radical-induced diseases. 
    

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