Cytotoxic Test of Fraction of Gaharu Leaves (Aquilaria Malaccensis) on Cervic Cancer Cells (Hela Cells) Using MTT Assay Method

Abstract

Cervical cancer is one type of cancer that attacks women in both developed and developing countries. Cancer can arise from cell division errors or DNA damage from certain environmental exposures that cause free radicals as a factor of cancer cells. The agarwood plant (Aquilaria malaccensis Lam) has been known by society as a health tea ingredient. The content of compounds contained in tea is believed to have anti-cancer and antioxidant activity. In previous studies found some of the active compound ingredients contained in the leaves of agarwood, such as alkaloids, flavonoids, triterpenoid, saponins and tannins. Tests that have been done previously that agarwood plants have good cytotoxic activity against cancer cells MCF-7 from one of the fractions, the n-hexane fraction. In this study, another cytotoxic potential test was carried out from aloe plants on other cancer cells, namely HeLa cells. Extraction was carried out by the Soxhletation method using 96% ethanol solvent, then fractionated with n-hexane solvent, ethyl asetate, and methanol: water. The MTT assay method is used to test the cytotoxic activity of sample research on cell cultures used. Measurements were performed using an ELISA reader at 550 nm wavelength. The results showed that the IC₅₀ values of the four samples tested were ethanol extract (299,506 µg / ml), n-hexane fraction (120,913 µg / ml), ethyl acetate fraction (3490,476 µg / ml) and methanol fraction: water (294,060 µg) / ml). from extracts and fractions it is known that the highest value is shown by the n-hexane fraction in the moderately active/moderate category.