Method development and validation of hydrochlorothiazide in tablet dosage form by UV spectroscopy

Abstract

A simple, reproducible and efficient spectroscopic method development and validation of hydrochlorothiazide in tablet dosage form. The drug was ermined by using methanol as a solvent for this study, which is determined by spectrophotometrically at 224-nm.the percentage recovery study of the drug for the proposed method range from 99-100%w/v indicating no interferences of the tablet excipients, by using methanol as a solvent. Linearity was obtained in the concentration range 10-50 μg/ml for the hydrochlorothiazide with correlation coefficient of 0.9916. The result analysis was validated statistically and recovery studies confirmed the accuracy and precision of the proposed method.