Synthesis and Evaluation of Anti-Depressant like Activity of Some Novel Thieno 1, 2, 3 – triazine 4 – ones

Abstract

Five derivatives of Thieno 1, 2, 3 triazine 4 – ones were synthesized by a well known Gewald reaction followed by Diazotization. The  chemical structures of the compounds were proved by Physical and Spectral data. Compounds were screened for its antidepressant  activity by using Tail Suspension Test (TST), Reserpine Induced Hypothermia and Forced Swim Test (FST). In Tail suspension test JSV 2a  (3- furfuryl–5,6–dihydrocyclohexathieno [2,3-d][1,2,3] triazine-4(3H)-one) and JSV 2b (3-cyclohexyl-5,6-dihydrocyclohexathieno [2,3-d] [1,2,3] triazine -4(3H)-one) showed significant activity (p<0.001) by decreasing immobility time of mice at higher dose levels (50mg/kg).  At lower dose levels (25 mg/kg) only JSV 2a had decreased the immobility time and shown significant activity (p<0.001), while JSV 2c (3-  furfuryl-5,6-dihydrocyclopentathieno [2,3-d][1,2,3] triazine -4(3H)-one), JSV 2d (3- cyclohexayl-5,6-dihydrocyclopentathieno [2,3-d][1,2,3] triazine -4(3H)-one) and JSV 2e (3-cyclohexayl-5,6-dimethylthieno [2,3-d][1,2,3] triazine -4(3H)-one) have not shown any activity at both  dose levels. JSV 2b at 25mg/kg did not show any activity. Based on the results of TST out of five only two compounds were used for  other two models as they have shown significant results. In Reserpine induced hypothermia test in Rats JSV 2a and JSV 2b at 50mg/kg  showed significant activity (p<0.001). At lower dose levels (25 mg/kg) only JSV 2a has shown significant activity (p<0.001). JSV 2b at  25mg/kg did not show any activity. In Forced Swim Test JSV 2a and JSV 2b at 50 mg/kg have shown significant activity (p<0.001). JSV 2a  at 25 mg/kg has shown significant activity (p<0.001) whereas; JSV 2b did not show any activity at 25 mg/kg. These results indicate that  compounds possessed Antidepressant activity. Anti-depressant activity of both the compounds at both the dose levels (25mg/kg and 
50mg/kg) was comparable to standard drug Imipramine (20mg/kg).