Assessment of Chromosome Cassette Pattern of MRSA in Correlation to Coagulase Gene

Salama Mostafa Abdel-Hafez (1) , Essam Hassan Mohamed (2) , Youssef Saeed Alghamdi (3) , Mohamed Mohamed Soliman (4) , Saad Hommod Alotaibi (5) , Adel Alkhedaide (6) , Magdy Yassin Hassan (7) , Hamada Hassan Amer (8)
(1) Department of Biology, Turabah University College, Taif University, Turabah-21995, Saudi Arabia, Saudi Arabia ,
(2) Department of Biology, Turabah University College, Taif University, Turabah-21995, Saudi Arabia, Saudi Arabia ,
(3) Department of Biology, Turabah University College, Taif University, Turabah-21995, Saudi Arabia, Saudi Arabia ,
(4) Cinical Laboratories Sciences, Turabah University College, Taif University, Turabah-21995, Saudi Arabia, Saudi Arabia ,
(5) Chemistry Department, Turabah University College, Turabah-29541, Taif University, Saudi Arabia, Saudi Arabia ,
(6) Cinical Laboratories Sciences, Turabah University College, Taif University, Turabah-21995, Saudi Arabia, Saudi Arabia ,
(7) Department of Biology, Turabah University College, Taif University, Turabah-21995, Saudi Arabia, Saudi Arabia ,
(8) Chemistry Department, Turabah University College, Turabah-29541, Taif University, Saudi Arabia, Saudi Arabia

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Vol. 12 No. 3 (2021): Volume 12 Issue 3
Keywords:
    MRSA, Chromosome Cassette Patterns, Coagulase Repeats, PCR
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Abstract

The resistance of methicillin-resistant Staphylococcus aureus (MRSA) to antimicrobials drugs is due to expression of the mecA gene. Current study was conducted on 33 MRSA clinical samples (Cefotaxime and Oxacillin positive). All MRSA isolates were examined using Antibiogram, Minimum Inhibitory concentration (MIC) and PCR to clarify the expression of SCCmec genes and to detect the differences on repeats of coagulase gene (Coa). Results showed that all isolates were 100% resistant against Amoxycillin-clavulanate, Ampicillin and Cefotaxime, 45.5% were resistant against Ciprofloxacin and erythromycin. mecA gene is expressed in all examined isolates (100%). All examined isolates harbored and expressed coagulase gene repeats. Coagulase repeats were 27.27% with 5 repeats (81pb), and 72.72% with 4 repeats. In conclusion, the virulence of MRSA strains is increased and gave different antibiogram activities from different global regions and the repeats of Coa gene give no detectable differences among MRSA strains.

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References

Batabyal, B., Kundu, G. K., Biswas, S. 2012. Methicillin-resistant Staphylococcus aureus: a brief review. Int. Res. J. Biological Sci, 1(7):65–71.

Beck, W. D., Berger-Bächi, B., Kayser, F. H. 1986. Additional DNA in methicillin-resistant Staphylococcus aureus and molecular cloning of mec-specific DNA. Journal of Bacteriology, 165(2):373–378.

de Sousa, M. A., de Lencastre, H. 2003. Evolution of Sporadic Isolates of Methicillin-Resistant Staphylococcus aureus (MRSA) in Hospitals and Their Similarities to Isolates of Community-Acquired MRSA. Journal of Clinical Microbiology, 41(8):3806–3815.

Deurenberg, R. H., Vink, C., Kalenic, S., Friedrich, A. W., Bruggeman, C. A., Stobberingh, E. E. 2007. The molecular evolution of methicillin-resistant Staphylococcus aureus. Clinical Microbiology and Infection, 13(3):222–235.

Goh, S. H., Byrne, S. K., Zhang, J. L., Chow, A. W. 1992. Molecular typing of Staphylococcus aureus on the basis of coagulase gene polymorphisms. Journal of Clinical Microbiology, 30(7):1642–1645.

Hanssen, A. M., Sollid, J. U. 2006. SCCmec in staphylococci: genes on the move. FEMS Immunology and Medical Microbiology, 46(1):8–20.

Hartman, B. J., Tomasz, A. 1984. Low-affinity penicillin-binding protein associated with beta-lactam resistance in Staphylococcus aureus. Journal of Bacteriology, 158(2):513–516.

Hesje, C. K., Sanfilippo, C. M., Haas, W., Morris, T. W. 2011. Molecular Epidemiology of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Isolated from the Eye. Current Eye Research, 36(2):94–102.

Hiramatsu, K., Cui, L., Kuroda, M., Ito, T. 2001. The emergence and evolution of methicillin-resistant Staphylococcus aureus. Trends in Microbiology, 9(10):486–493.

Holtfreter, S., Grumann, D., Schmudde, M., Nguyen, H. T. T., Eichler, P., Strommenger, B., Kopron, K., Kolata, J., Giedrys-Kalemba, S., Steinmetz, I., Witte, W., Broker, B. M. 2007. Clonal Distribution of Superantigen Genes in Clinical Staphylococcus aureus Isolates. Journal of Clinical Microbiology, 45(8):2669–2680.

Ito, T., Ma, X. X., Takeuchi, F., Okuma, K., Yuzawa, H., Hiramatsu, K. 2004. Novel Type V Staphylococcal Cassette Chromosome mec Driven by a Novel Cassette Chromosome Recombinase, ccrC. Antimicrobial Agents and Chemotherapy, 48(7):2637–2651.

Javid, F., Taku, A., Bhat, M. A., Badroo, G. A., Mudasir, M., Sofi, T. A. 2018. Molecular typing of Staphylococcus aureus based on coagulase gene. Veterinary World, 11(4):423–430.

Lodise, T. P., McKinnon, P. S. 2005. Clinical and economic impact of methicillin resistance in patients with Staphylococcus aureus bacteremia. Diagnostic Microbiology and Infectious Disease, 52(2):113–122.

Ma, X. X., Ito, T., Tiensasitorn, C., Jamklang, M., Chongtrakool, P., Boyle-Vavra, S., Daum, R. S., Hiramatsu, K. 2002. Novel Type of Staphylococcal Cassette Chromosome mec Identified in Community-Acquired Methicillin-Resistant Staphylococcus aureus Strains. Antimicrobial Agents and Chemotherapy, 46(4):1147–1152.

Maree, C. L., Daum, R. S., Boyle-Vavra, S., Matayoshi, K., Miller, L. G. 2007. Community-associated Methicillin-resistant Staphylococcus aureus Isolates and Healthcare-Associated Infections1. Emerging Infectious Diseases, 13(2):236–242.

Miller, L. G., Perdreau-Remington, F., Rieg, G., Mehdi, S., Perlroth, J., Bayer, A. S., Tang, A. W., Phung, T. O., Spellberg, B. 2005. Necrotizing Fasciitis Caused by Community-Associated Methicillin-Resistant Staphylococcus aureusin Los Angeles. New England Journal of Medicine, 352(14):1445–1453.

Murchan, S., Kaufmann, M. E., Deplano, A., de Ryck, R., Struelens, M., Zinn, C. E., Fussing, V., Salmenlinna, S., Vuopio-Varkila, J., Solh, N. E., Cuny, C., Witte, W., Tassios, P. T., Legakis, N., van Leeuwen, W., van Belkum, A., Vindel, A., Laconcha, I., Garaizar, J., Haeggman, S., Olsson-Liljequist, B., Ransjo, U., Coombes, G., Cookson, B. 2003. Harmonization of Pulsed-Field Gel Electrophoresis Protocols for Epidemiological Typing of Strains of Methicillin-Resistant Staphylococcus aureus: a Single Approach Developed by Consensus in 10 European Laboratories and Its Application for Tracing the Spread of Related Strains. Journal of Clinical Microbiology, 41(4):1574–1585.

Nimmo, G. R., Coombs, G. W., Pearson, J. C., Brien, F. G. O., Christiansen, K. J., Turnidge, J. D., Gosbell, I. B., Collignon, P., McLaws, M.-L. 2006. Methicillin-resistant Staphylococcus aureus in the Australian community: an evolving epidemic. Medical Journal of Australia, 184(8):384–388.

Okuma, K., Iwakawa, K., Turnidge, J. D., Grubb, W. B., Bell, J. M., O’Brien, F. G., Coombs, G. W., Pearman, J. W., Tenover, F. C., Kapi, M., Tiensasitorn, C., Ito, T., Hiramatsu, K. 2002. Dissemination of New Methicillin-Resistant Staphylococcus aureus Clones in the Community. Journal of Clinical Microbiology, 40(11):4289–4294.

Omar, N. Y., Ali, H. A. S., Harfoush, R. A. H., Khayat, E. H. E. 2014. Molecular Typing of Methicillin Resistant Staphylococcus aureus Clinical Isolates on the Basis of Protein A and Coagulase Gene Polymorphisms. International Journal of Microbiology, 2014:1–11.

Palma, M., Shannon, O., Quezada, H. C., Berg, A., Flock, J.-I. 2001. Extracellular Fibrinogen-binding Protein, Efb, from Staphylococcus aureus-Blocks Platelet Aggregation Due to Its Binding to the α-Chain. Journal of Biological Chemistry, 276(34):31691–31697.

Pandey, S., Raza, M. S., Bhatta, C. P. 2013. Prevalence and Antibiotic Sensitivity Pattern of Methicillin- Resistant-Staphylococcus aureus in Kathmandu Medical College-Teaching Hospital. Journal of Institute of Medicine Nepal, 34(1):13–17.

Sadeghi, J., Mansouri, S. 2014. Molecular characterization and antibiotic resistance of clinical isolates of methicillin-resistant Staphylococcus aureus obtained from Southeast of Iran (Kerman). APMIS, 122(5):405–411.

Sanjiv, K., Kataria, A. K., Sharma, R., Singh, G. J. V. 2008. Epidemiological typing of Staphylococcus aureus by DNA restriction fragment length polymorphism of coa gene. Veterinarski Arhiv, 78(1):31–38.

Shore, A. C., Coleman, D. C. 2013. Staphylococcal cassette chromosome mec: Recent advances and new insights. International Journal of Medical Microbiology, 303(6-7):350–359.

van Hal, S. J., Stark, D., Lockwood, B., Marriott, D., Harkness, J. 2007. Methicillin-Resistant Staphylococcus aureus (MRSA) Detection: Comparison of Two Molecular Methods (IDI-MRSA PCR Assay and Geno Type MRSA Direct PCR Assay) with Three Selective MRSA Agars (MRSA ID, MRSASelect, and CHROMagar MRSA) for Use with Infection-Control Swabs. Journal of Clinical Microbiology, 45(8):2486–2490.

Wisplinghoff, H., Rosato, A. E., Enright, M. C., Noto, M., Craig, W., Archer, G. L. 2003. Related Clones Containing SCCmec Type IV Predominate among Clinically Significant Staphylococcus epidermidis Isolates. Antimicrobial Agents and Chemotherapy, 47(11):3574–3579.

Wu, Z., Li, F., Liu, D., Xue, H., Zhao, X. 2015. Novel Type XII Staphylococcal Cassette Chromosome mec Harboring a New Cassette Chromosome Recombinase, CcrC2. Antimicrobial Agents and Chemotherapy, 59(12):7597–7601.

Authors

Salama Mostafa Abdel-Hafez
Department of Biology, Turabah University College, Taif University, Turabah-21995, Saudi Arabia
drsalamahafez@gmail.com (Primary Contact)
Essam Hassan Mohamed
Department of Biology, Turabah University College, Taif University, Turabah-21995, Saudi Arabia
Youssef Saeed Alghamdi
Department of Biology, Turabah University College, Taif University, Turabah-21995, Saudi Arabia
Mohamed Mohamed Soliman
Cinical Laboratories Sciences, Turabah University College, Taif University, Turabah-21995, Saudi Arabia
Saad Hommod Alotaibi
Chemistry Department, Turabah University College, Turabah-29541, Taif University, Saudi Arabia
Adel Alkhedaide
Cinical Laboratories Sciences, Turabah University College, Taif University, Turabah-21995, Saudi Arabia
Magdy Yassin Hassan
Department of Biology, Turabah University College, Taif University, Turabah-21995, Saudi Arabia
Hamada Hassan Amer
Chemistry Department, Turabah University College, Turabah-29541, Taif University, Saudi Arabia
Salama Mostafa Abdel-Hafez, Essam Hassan Mohamed, Youssef Saeed Alghamdi, Mohamed Mohamed Soliman, Saad Hommod Alotaibi, Adel Alkhedaide, Magdy Yassin Hassan, & Hamada Hassan Amer. (2021). Assessment of Chromosome Cassette Pattern of MRSA in Correlation to Coagulase Gene. International Journal of Research in Pharmaceutical Sciences, 12(3), 2207–2214. Retrieved from https://ijrps.com/home/article/view/257
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