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\def\RunningAuthor{Raghavendra Shanbhog }
\firstPage{7716}
\articleType{Original Article}
\receivedDate{01 Oct 2020}
\acceptedDate{09 Nov 2020}
\revisedDate{02 Nov 2020}
\journalVolume{2020, 11}
\journalIssue{4}
\journalDoi{ijrps.v11i4.4123}
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\title{\textbf{Prevalence of dental caries and Clinical sequel of untreated dental caries evaluated with PUFA index among school going children in the rural Mysuru Taluk, Karnataka}}
\author{Raghavendra~Shanbhog\textsuperscript{*}\textsuperscript{1},  
            Srilatha~K~T\textsuperscript{1},  
            Madhushree~B\textsuperscript{2},  
            Manjunatha~S~N\textsuperscript{3}, 
            Chanchala~H~P\textsuperscript{1}~\\[5pt]{\textsuperscript{1}Department of Pediatric and Preventive Dentistry, JSS Dental College and Hospital, JSSAHER, Mysuru, Karnataka, India }~\\{\textsuperscript{2}Department of  Public Health and Preventive Medicine, JSS Medical College and Hospital, JSSAHER, Mysuru, Karnataka, India }~\\{\textsuperscript{3}Department of Public Health and Preventive Medicine, Mysuru Medical College and Hospital, Mysuru, Karnataka, India}}

\begin{abstract}
In literature real time monitoring of dental caries in rural parts of India are lacking. This warrants periodic prevalence studies to plan and implement oral health programs. So the present study aims at evaluating the dental caries status among rural population in Mysuru Taluk, of Karnataka. A cross-sectional study was conducted among 1800 children with recruited populations at varying age levels of three population groups 4-6, 8-10 and 12-14 years. Data regarding oral health status (DMFT, deft, PUFA, pufa) were collected by type-III clinical examinations. The collected data were administered statistically. In 8-10 years children permanent teeth caries prevalence was found to be 54.3\% which increased to 67.8 \% in12-14 years. Among 4-6 years children primary teeth caries prevalence was found to be 47.4\% which increased to 73.2\% in 8-10 years. Among 4-6 and 12-14 years children percentage prevalence of PUFA was 32.2\% which increased to 52.3\% in 8-10 years. No clinically significant variation was observed in Caries prevalence among gender. The study outcome revealed oral health condition in children of rural Mysuru was neglected. High prevalence of dental caries was observed among Children with low dental care utilization.  
\end{abstract}\def\keywordstitle{Keywords}

\begin{keywords}Rural Mysuru,\newline Dental caries,\newline PUFA Index,\newline Caries Prevalence,\newline Children
\end{keywords}
\twocolumn[ \maketitle {\printKwdAbsBox}]
\makeatletter{\raggedright\textsuperscript{*}Corresponding Author\par Name:\ Raghavendra~Shanbhog~\\ Phone:\ +91-9945112080~\\  Email:\ drraghu.kiddoc@gmail.com
        \par}\vspace*{-11pt}\hrulefill\par{\fontsize{12}{14}\selectfont ISSN: 0975-7538}\par% 
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\section{Introduction}
Dental caries is a biofilm based, diet controlled, multifactorial, non-communicable, active disease resulting in gross mineral loss of dental hard tissues. It is mediated by factors like biological, behavioral, psychosocial, and environmental\unskip~\citep{980677:21196240,980677:21196369}. Un-treated dental caries with related discomfort will impact quality of life, the cognitive development and growth parameters in young children\unskip~\citep{980677:21196372,980677:21196353}.

In past 25 years the trends of dental caries in Indian children revealed average pooled caries prevalence of 56.7\%, 48.9\%, 69.1\%, and 52.1\% for 2{\textendash}5, 6{\textendash}10, and 11{\textendash}15 year, of age-group, correspondingly. It is also observed that one out of two children in India are affected by dental caries with weighed mean caries experience of 2.3\unskip~\citep{980677:21196361}.

Urban and rural population of India showed a vast difference in health status both General and oral health Rural Indian oral health care facilities are inadequate due to shortage of manpower, financial confines and the lack of perceived need and knowledge among rural population\unskip~\citep{980677:21196351,980677:21196348}. A critical analyses done on ample dental literature which is available about caries levels in the Indian population showed maximum data have been acquired from the metro cities and cosmopolitan areas. However, there is no appropriate and satisfactory data as yet regarding the dental caries status in the rural areas of India\unskip~\citep{980677:21196373}. Also in literature a large number of surveys have been conducted reporting the prevalence of dental caries among schoolchildren in India, but the data on severity and clinical sequel of untreated dental caries are relatively unknown.

Lack of real time monitoring of dental caries in rural parts of India warrants an urgent need to assess the prevalence for planning and execution of oral health programs. The present study aims at evaluating the dental caries prevalence among the rural population in Mysuru Taluk, of Karnataka.

\textbf{Methodology}

\textbf{Ethical clearance}

The study protocol was reviewed and approved by the Institutional Research Ethics Committee. After explaining the study procedure permission was obtained from concerned government and school authority. Written informed consent was acquired from the parents of children who participated in the study.

\textbf{Study design and location }

The present study was a cross-sectional survey conducted in Mysuru of south India falling in the survey of India degree sheet Nos. 48P, 57D, 57H and 58A. Mysuru is bound by north latitudes 11045' - 12040' and east longitudes 75059' -77005' covering 6269 Sq. km. The present study was undertaken in 35 villages of Mysuru Taluk.

\textbf{Study sample }

Study sample comprised of consented children (4-6, 8-10 and 12-14 years), free of systemic illness, attending anganwadi centers and government schools along with parents. Schools children were targeted for high, anticipated levels of cooperation and low population mobility. Subjects were selected with a view to recruiting children at varying age levels of three population groups 4-6, 8-10 and 12-14 years. Equal number of subjects between the subject groups with the configuration of recruitment was scrutinized to reduce imbalance between the subject groups.

\textbf{Sample and Sampling Technique}

Based on literature search Caries Prevalence of rural India shows the caries prevalence of 40 to 80\% in rural India\unskip~\citep{980677:21196351,980677:21196348,980677:21196373,980677:21196363}. The sample size was calculated based on the prevalence rate of dental caries to be 60\% with 4\% type I~error and 90\% power of the study the sample size was estimated to be 576 children which were rounded off to 600 in each age group. As we had recruited populations at varying age levels of three population groups 4-6, 8-10 and 12-14 years total sample size will be 1800 child along with parent.

\textbf{Clinical examination }

Children were examined in the school sites with the students seated. Natural day light was used for illumination. Oral clinical examination was accomplished by three trained and calibrated pediatric dentists. Caries was analyzed as per the criteria established by the World Health Organization \unskip~\citep{980677:21196357}  using a mouth mirror and a community periodontal index (CPI) probe. Caries was recorded for both permanent and primary teeth in terms of decayed, missing and filled teeth index (DMFT and dmft), as per World Health Organization recommendations for oral health surveys (1997) \unskip~\citep{980677:21196357}  and untreated caries was assessed using PUFA/pufa index according to the standard procedure protocol recommended by \unskip~\citet{980677:21196366}.


\begin{table*}[!htbp]
\caption{\boldmath {Distribution of study subjects according to age and sex} }
\label{tw-f0970a3069be}
\centering 
\begin{threeparttable}

\def\arraystretch{1.1}
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\begin{tabulary}{\linewidth}{p{\dimexpr.1953\linewidth-2\tabcolsep}p{\dimexpr.19909999999999997\linewidth-2\tabcolsep}p{\dimexpr.20970000000000002\linewidth-2\tabcolsep}p{\dimexpr.20700000000000003\linewidth-2\tabcolsep}p{\dimexpr.1889\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}\multicolumn{2}{p{\dimexpr(.3944\linewidth-2\tabcolsep)}}{\cAlignHack Age in years} & \multicolumn{2}{p{\dimexpr(.4167\linewidth-2\tabcolsep)}}{\cAlignHack Gender} & Total\\
\rowcolor{kwdboxcolor} &  & Male & Female & \\
\tblmidrule 
\multicolumn{1}{p{\dimexpr(.1953\linewidth-2\tabcolsep)}}{\multirow{3}{\linewidth}{4-6}} &
  N &
  300 &
  300 &
  600\\
 &
  \% &
  50.0\% &
  50.0\% &
  100.0\%\\
 &
  \% of Total &
  16.7\% &
  16.7\% &
  33.3\%\\
\multicolumn{1}{p{\dimexpr(.1953\linewidth-2\tabcolsep)}}{\multirow{3}{\linewidth}{8-10}} &
  N &
  302 &
  298 &
  600\\
 &
  \% &
  50.3\% &
  49.7\% &
  100.0\%\\
 &
  \% of Total &
  16.8\% &
  16.6\% &
  33.3\%\\
\multicolumn{1}{p{\dimexpr(.1953\linewidth-2\tabcolsep)}}{\multirow{3}{\linewidth}{12-14}} &
  N &
  282 &
  318 &
  600\\
 &
  \% &
  47.0\% &
  53.0\% &
  100.0\%\\
 &
  \% of Total &
  15.7\% &
  17.7\% &
  33.3\%\\
\multicolumn{1}{p{\dimexpr(.1953\linewidth-2\tabcolsep)}}{\multirow{3}{\linewidth}{Total}} &
  N &
  884 &
  916 &
  1800\\
 &
  \% &
  49.1\% &
  50.9\% &
  100.0\%\\
 &
  \% of Total &
  49.1\% &
  50.9\% &
  100.0\%\\
\tblbottomrule 
\end{tabulary}\par 
\begin{tablenotes}\footnotesize 
    
\item{Pearson Chi-Square-1.618, p-0.445}
\end{tablenotes}
\end{threeparttable}

\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {Distribution of study subjects according to Socio-demographic variable} }
\label{tw-be991301376f}
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\tblmidrule 
Primary (1-IX) &
  \cAlignHack 1102 &
  \cAlignHack 61.2\\
Secondary(X) &
  \cAlignHack 410 &
  \cAlignHack 22.8\\
Higher secondary(XII) &
  \cAlignHack 288 &
  \cAlignHack 16.0\\
Graduate &
  \cAlignHack 0 &
  \cAlignHack 0\\
Postgraduate and higher &
  \cAlignHack 0 &
  \cAlignHack 0\\
Total &
  \cAlignHack 1800 &
  \cAlignHack 100.0\\
\textbf{Occupation} &
  \cAlignHack \textbf{N} &
  \cAlignHack \textbf{\%}\\
White collar &
  \cAlignHack 0 &
  \cAlignHack 0\\
Pink collar &
  \cAlignHack 381 &
  \cAlignHack 21.2\\
Blue collar &
  \cAlignHack 1419 &
  \cAlignHack 78.8\\
Total &
  \cAlignHack 1800 &
  \cAlignHack 100.0\\
\textbf{Socioeconomic Status} &
  \cAlignHack \textbf{N} &
  \cAlignHack \textbf{\%}\\
BPL card holder &
  \cAlignHack 1075 &
  \cAlignHack 59.7\\
Non-BPL card holder &
  \cAlignHack 725 &
  \cAlignHack 40.3\\
Total &
  \cAlignHack 1800 &
  \cAlignHack 100.0\\
\tblbottomrule 
\end{tabulary}\par 
\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {Distribution of study subjects according DMFT to age and Gender} }
\label{tw-7acd18e60597}
\centering 
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\tbltoprule \rowcolor{kwdboxcolor}\multicolumn{2}{p{\dimexpr(.1836\linewidth-2\tabcolsep)}}{\cAlignHack Age in years } & \multicolumn{7}{p{\dimexpr(.7015\linewidth-2\tabcolsep)}}{\cAlignHack Permanent Teeth Decay Component} & \cAlignHack Total\\
\tblmidrule 
 &
   &
  \textbf{0} &
  \textbf{2.00} &
  \textbf{3.00} &
  \textbf{4.00} &
  \textbf{6.00} &
  \textbf{7.00} &
  \textbf{8.00} &
  \\
\multicolumn{1}{p{\dimexpr(.104\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{4 to 6}} &
  N &
  599 &
  1 &
  0 &
  0 &
  0 &
  0 &
  0 &
  600\\
 &
  \% &
  99.8\% &
  0.2\% &
  0.0\% &
  0.0\% &
  0.0\% &
  0.0\% &
  0.0\% &
  100.0\%\\
\multicolumn{1}{p{\dimexpr(.104\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{8 to 10}} &
  N &
  274 &
  127 &
  85 &
  94 &
  0 &
  20 &
  0 &
  600\\
 &
  \% &
  45.7\% &
  21.2\% &
  14.2\% &
  15.7\% &
  0.0\% &
  3.3\% &
  0.0\% &
  100.0\%\\
\multicolumn{1}{p{\dimexpr(.104\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{12 to 14}} &
  N &
  193 &
  88 &
  108 &
  143 &
  12 &
  50 &
  6 &
  600\\
 &
  \% &
  32.2\% &
  14.7\% &
  18.0\% &
  23.8\% &
  2.0\% &
  8.3\% &
  1.0\% &
  100.0\%\\
\multicolumn{1}{p{\dimexpr(.104\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{Total}} &
  N &
  1066 &
  216 &
  193 &
  237 &
  12 &
  70 &
  6 &
  1800\\
 &
  \% &
  59.2\% &
  12.0\% &
  10.7\% &
  13.2\% &
  0.7\% &
  3.9\% &
  0.3\% &
  100.0\%\\
\multicolumn{2}{p{\dimexpr(.1836\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{\textbf{Gender }}} &
  \multicolumn{7}{p{\dimexpr(.7015\linewidth-2\tabcolsep)}}{\cAlignHack \textbf{Permanent Decay Component}} &
  \multicolumn{1}{p{\dimexpr(.11489999999999998\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{\textbf{Total}}}\\
 &
   &
  \textbf{0} &
  \textbf{2.00} &
  \textbf{3.00} &
  \textbf{4.00} &
  \textbf{6.00} &
  \textbf{7.00} &
  \textbf{8.00} &
  \\
\multicolumn{1}{p{\dimexpr(.104\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{Male}} &
  N &
  527 &
  110 &
  101 &
  105 &
  8 &
  31 &
  2 &
  884\\
 &
  \% &
  59.6\% &
  12.4\% &
  11.4\% &
  11.9\% &
  0.9\% &
  3.5\% &
  0.2\% &
  100.0\%\\
\multicolumn{1}{p{\dimexpr(.104\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{Female}} &
  N &
  539 &
  106 &
  92 &
  132 &
  4 &
  39 &
  4 &
  916\\
 &
  \% &
  58.8\% &
  11.6\% &
  10.0\% &
  14.4\% &
  0.4\% &
  4.3\% &
  0.4\% &
  100.0\%\\
\multicolumn{1}{p{\dimexpr(.104\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{Total}} &
  N &
  1066 &
  216 &
  193 &
  237 &
  12 &
  70 &
  6 &
  1800\\
 &
  \% &
  59.2\% &
  12.0\% &
  10.7\% &
  13.2\% &
  0.7\% &
  3.9\% &
  0.3\% &
  100.0\%\\
\tblbottomrule 
\end{tabulary}\par 
\begin{tablenotes}\footnotesize 
    
\item{Age - Pearson Chi-Square- 704.604, p-0.001  Gender - Pearson Chi-Square-6.052, p-0.417}
\end{tablenotes}
\end{threeparttable}

\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {Comparison of the mean DMFT and deft with respect to age and gender} }
\label{tw-4004fe9d3ca2}
\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.2515\linewidth-2\tabcolsep}p{\dimexpr.1485\linewidth-2\tabcolsep}p{\dimexpr.20\linewidth-2\tabcolsep}p{\dimexpr.20\linewidth-2\tabcolsep}p{\dimexpr.20\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor} & \multicolumn{2}{p{\dimexpr(.3485\linewidth-2\tabcolsep)}}{\cAlignHack Permanent teeth} & \multicolumn{2}{p{\dimexpr(.40\linewidth-2\tabcolsep)}}{\cAlignHack Primary teeth}\\
\tblmidrule 
Age in years  &
  \cAlignHack Mean &
  \cAlignHack SD &
  \cAlignHack Mean &
  \cAlignHack SD\\
4 to 6  &
  \cAlignHack 0.03 &
  \cAlignHack 0.08 &
  \cAlignHack 1.30 &
  \cAlignHack 2.06\\
8 to 10  &
  \cAlignHack 1.71 &
  \cAlignHack 1.83 &
  \cAlignHack 2.99 &
  \cAlignHack 2.66\\
12 to 14  &
  \cAlignHack 2.57 &
  \cAlignHack 2.21 &
  \cAlignHack 0.05 &
  \cAlignHack 0.23\\
 &
  \multicolumn{2}{p{\dimexpr(.3485\linewidth-2\tabcolsep)}}{\cAlignHack \textbf{Permanent teeth}} &
  \multicolumn{2}{p{\dimexpr(.40\linewidth-2\tabcolsep)}}{\cAlignHack \textbf{Primary teeth}}\\
Gender  &
  \cAlignHack Mean &
  \cAlignHack SD &
  \cAlignHack Mean &
  \cAlignHack SD\\
Male  &
  \cAlignHack 1.38 &
  \cAlignHack 1.92 &
  \cAlignHack 1.38 &
  \cAlignHack 2.27\\
Female &
  \cAlignHack 1.47 &
  \cAlignHack 2.01 &
  \cAlignHack 1.50 &
  \cAlignHack 2.30\\
\tblbottomrule 
\end{tabulary}\par 
\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {Distribution of study subjects according deft toage and Gender} }
\label{tw-6d260d2659e4}
\centering 
\begin{threeparttable}

\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.08530000000000001\linewidth-2\tabcolsep}p{\dimexpr.0437\linewidth-2\tabcolsep}p{\dimexpr.06539999999999999\linewidth-2\tabcolsep}p{\dimexpr.07140000000000001\linewidth-2\tabcolsep}p{\dimexpr.06619999999999999\linewidth-2\tabcolsep}p{\dimexpr.05210000000000001\linewidth-2\tabcolsep}p{\dimexpr.0584\linewidth-2\tabcolsep}p{\dimexpr.0572\linewidth-2\tabcolsep}p{\dimexpr.0588\linewidth-2\tabcolsep}p{\dimexpr.0634\linewidth-2\tabcolsep}p{\dimexpr.05380000000000001\linewidth-2\tabcolsep}p{\dimexpr.0589\linewidth-2\tabcolsep}p{\dimexpr.0651\linewidth-2\tabcolsep}p{\dimexpr.0763\linewidth-2\tabcolsep}p{\dimexpr.05930000000000001\linewidth-2\tabcolsep}p{\dimexpr.06470000000000001\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}Age in years  &  & \multicolumn{7}{p{\dimexpr(.4295\linewidth-2\tabcolsep)}}{\cAlignHack Primary Teeth Decay Component} &  &  &  &  &  &  & \cAlignHack Total\\
\tblmidrule 
 &
   &
  \textbf{0} &
  \textbf{1.00} &
  \textbf{2.00} &
  \textbf{3.00} &
  \textbf{4.00} &
  \textbf{5.00} &
  \textbf{6.00} &
  \textbf{7.00 } &
  \textbf{8.00 } &
  \textbf{9.00 } &
  \textbf{10.00 } &
  \textbf{11.00 } &
  \textbf{12.00} &
  \\
\multicolumn{1}{p{\dimexpr(.08530000000000001\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{4 to 6}} &
  N &
  316 &
  87 &
  135 &
  1 &
  1 &
  4 &
  5 &
  46 &
  4 &
  0 &
  1 &
  0 &
  0 &
  \cAlignHack 600\\
 &
  \% &
  52.7 &
  14.5 &
  22.5 &
  0.2 &
  0.2 &
  0.7 &
  0.8 &
  7.7 &
  0.7 &
  0.0 &
  0.2 &
  0.0 &
  0.0 &
  \cAlignHack 100.0\%\\
 &
   &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \\
\multicolumn{1}{p{\dimexpr(.08530000000000001\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{8 to 10}} &
  N &
  161 &
  57 &
  77 &
  59 &
  81 &
  44 &
  49 &
  33 &
  27 &
  4 &
  5 &
  2 &
  1 &
  \cAlignHack 600\\
 &
  \% &
  26.8 &
  9.5 &
  12.8 &
  9.8 &
  13.5 &
  7.3 &
  8.2 &
  5.5 &
  4.5 &
  0.7 &
  0.8 &
  0.3 &
  0.2 &
  \cAlignHack 100.0\%\\
 &
   &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \\
\multicolumn{1}{p{\dimexpr(.08530000000000001\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{12 to 14}} &
  N &
  574 &
  24 &
  2 &
  0 &
  0 &
  0 &
  0 &
  0 &
  0 &
  0 &
  0 &
  0 &
  0 &
  \cAlignHack 600\\
 &
  \% &
  95.7 &
  4.0 &
  0.3 &
  0.0 &
  0.0 &
  0.0 &
  0.0 &
  0.0 &
  0.0 &
  0.0 &
  0.0 &
  0.0 &
  0.0 &
  \cAlignHack 100.0\%\\
 &
   &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \\
\multicolumn{1}{p{\dimexpr(.08530000000000001\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{Total}} &
  N &
  1051 &
  168 &
  214 &
  60 &
  82 &
  48 &
  54 &
  79 &
  31 &
  4 &
  6 &
  2 &
  1 &
  \cAlignHack 1800\\
 &
  \% &
  58.4 &
  9.3 &
  11.9 &
  3.3 &
  4.6 &
  2.7 &
  3.0 &
  4.4 &
  1.7 &
  0.2 &
  0.3 &
  0.1 &
  0.1 &
  \cAlignHack 100.0\%\\
 &
   &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \\
\multicolumn{2}{p{\dimexpr(.12900000000000002\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{\textbf{Gender }}} &
  \multicolumn{7}{p{\dimexpr(.4295\linewidth-2\tabcolsep)}}{\cAlignHack \textbf{Primary Teeth Decay Component}} &
   &
   &
   &
   &
   &
   &
  \multicolumn{1}{p{\dimexpr(.06470000000000001\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{\cAlignHack \textbf{Total}}}\\
 &
   &
  \textbf{0} &
  \textbf{1.00} &
  \textbf{2.00} &
  \textbf{3.00} &
  \textbf{4.00} &
  \textbf{5.00} &
  \textbf{6.00} &
  \textbf{7.00 } &
  \textbf{8.00 } &
  \textbf{9.00 } &
  \textbf{10.00 } &
  \textbf{11.00 } &
  \textbf{12.00} &
  \\
\multicolumn{1}{p{\dimexpr(.08530000000000001\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{Male}} &
  N &
  511 &
  112 &
  91 &
  37 &
  32 &
  24 &
  24 &
  22 &
  21 &
  2 &
  5 &
  2 &
  1 &
  \cAlignHack 884\\
 &
  \% &
  57.8 &
  12.7 &
  10.3 &
  4.2 &
  3.6 &
  2.7 &
  2.7 &
  2.5 &
  2.4 &
  0.2 &
  0.6 &
  0.2 &
  0.1 &
  \cAlignHack 100.0\%\\
 &
   &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \\
\multicolumn{1}{p{\dimexpr(.08530000000000001\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{Female}} &
  N &
  540 &
  56 &
  123 &
  23 &
  50 &
  24 &
  30 &
  57 &
  10 &
  2 &
  1 &
  0 &
  0 &
  \cAlignHack 916\\
 &
  \% &
  59.0 &
  6.1 &
  13.4 &
  2.5 &
  5.5 &
  2.6 &
  3.3 &
  6.2 &
  1.1 &
  0.2 &
  0.1 &
  0.0 &
  0.0 &
  \cAlignHack 100.0\%\\
 &
   &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \\
\multicolumn{1}{p{\dimexpr(.08530000000000001\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{Total}} &
  N &
  1051 &
  168 &
  214 &
  60 &
  82 &
  48 &
  54 &
  79 &
  31 &
  4 &
  6 &
  2 &
  1 &
  \cAlignHack 1800\\
 &
  \% &
  58.4 &
  9.3 &
  11.9 &
  3.3 &
  4.6 &
  2.7 &
  3.0 &
  4.4 &
  1.7 &
  0.2 &
  0.3 &
  0.1 &
  0.1 &
  \cAlignHack 100.0\%\\
 &
   &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \% &
  \\
\tblbottomrule 
\end{tabulary}\par 
\begin{tablenotes}\footnotesize 
    
\item{Age Pearson Chi-Square- 940.370, p-0.001 Gender Pearson Chi-Square- 56.662, p-0.001}
\end{tablenotes}
\end{threeparttable}

\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {Distribution of study subjects according PUFA+pufa to age and Gender} }
\label{tw-717ece3bbaba}
\centering 
\begin{threeparttable}

\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.0971\linewidth-2\tabcolsep}p{\dimexpr.05979999999999999\linewidth-2\tabcolsep}p{\dimexpr.07350000000000001\linewidth-2\tabcolsep}p{\dimexpr.08489999999999998\linewidth-2\tabcolsep}p{\dimexpr.0629\linewidth-2\tabcolsep}p{\dimexpr.06879999999999999\linewidth-2\tabcolsep}p{\dimexpr.0738\linewidth-2\tabcolsep}p{\dimexpr.07580000000000001\linewidth-2\tabcolsep}p{\dimexpr.0584\linewidth-2\tabcolsep}p{\dimexpr.06300000000000001\linewidth-2\tabcolsep}p{\dimexpr.07510000000000001\linewidth-2\tabcolsep}p{\dimexpr.06869999999999999\linewidth-2\tabcolsep}p{\dimexpr.07630000000000001\linewidth-2\tabcolsep}p{\dimexpr.0619\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}\multicolumn{2}{p{\dimexpr(.1569\linewidth-2\tabcolsep)}}{\cAlignHack Age in years } & \multicolumn{7}{p{\dimexpr(.4981\linewidth-2\tabcolsep)}}{\cAlignHack PUFA} &  &  &  &  & Total\\
\tblmidrule 
 &
   &
  \textbf{.00} &
  \textbf{1.00} &
  \textbf{2.00} &
  \textbf{3.00} &
  \textbf{4.00} &
  \textbf{5.00} &
  \textbf{6.00} &
  \textbf{7.00} &
  \textbf{8.00} &
  \textbf{10.00} &
  \textbf{12.00} &
  \\
\multicolumn{1}{p{\dimexpr(.0971\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{4 to 6}} &
  N &
  407 &
  87 &
  46 &
  1 &
  2 &
  2 &
  6 &
  45 &
  3 &
  1 &
  0 &
  600\\
 &
  \% &
  67.8\% &
  14.5\% &
  7.7\% &
  0.2\% &
  0.3\% &
  0.3\% &
  1.0\% &
  7.5\% &
  0.5\% &
  0.2\% &
  0.0\% &
  100.0\%\\
\multicolumn{1}{p{\dimexpr(.0971\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{8 to 10}} &
  N &
  286 &
  56 &
  28 &
  49 &
  113 &
  36 &
  17 &
  4 &
  8 &
  1 &
  2 &
  600\\
 &
  \% &
  47.7\% &
  9.3\% &
  4.7\% &
  8.2\% &
  18.8\% &
  6.0\% &
  2.8\% &
  0.7\% &
  1.3\% &
  0.2\% &
  0.3\% &
  100.0\%\\
\multicolumn{1}{p{\dimexpr(.0971\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{12 to 14}} &
  N &
  407 &
  58 &
  12 &
  34 &
  59 &
  13 &
  9 &
  3 &
  5 &
  0 &
  0 &
  600\\
 &
  \% &
  67.8\% &
  9.7\% &
  2.0\% &
  5.7\% &
  9.8\% &
  2.2\% &
  1.5\% &
  0.5\% &
  0.8\% &
  0.0\% &
  0.0\% &
  100.0\%\\
\multicolumn{1}{p{\dimexpr(.0971\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{Total}} &
  N &
  1100 &
  201 &
  86 &
  84 &
  174 &
  51 &
  32 &
  52 &
  16 &
  2 &
  2 &
  1800\\
 &
  \% &
  61.1\% &
  11.2\% &
  4.8\% &
  4.7\% &
  9.7\% &
  2.8\% &
  1.8\% &
  2.9\% &
  0.9\% &
  0.1\% &
  0.1\% &
  100.0\%\\
\multicolumn{2}{p{\dimexpr(.1569\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{\textbf{Gender}}} &
  \multicolumn{7}{p{\dimexpr(.4981\linewidth-2\tabcolsep)}}{\cAlignHack \textbf{PUFA}} &
   &
   &
   &
   &
  \multicolumn{1}{p{\dimexpr(.0619\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{\textbf{Total}}}\\
 &
   &
  \textbf{.00} &
  \textbf{1.00} &
  \textbf{2.00} &
  \textbf{3.00} &
  \textbf{4.00} &
  \textbf{5.00} &
  \textbf{6.00} &
  \textbf{7.00} &
  \textbf{8.00} &
  \textbf{10.00} &
  \textbf{12.00} &
  \\
\multicolumn{1}{p{\dimexpr(.0971\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{Male}} &
  N &
  564 &
  87 &
  47 &
  40 &
  89 &
  32 &
  16 &
  4 &
  5 &
  0 &
  0 &
  884\\
 &
  \% &
  63.8\% &
  9.8\% &
  5.3\% &
  4.5\% &
  10.1\% &
  3.6\% &
  1.8\% &
  0.5\% &
  0.6\% &
  0.0\% &
  0.0\% &
  100.0\%\\
\multicolumn{1}{p{\dimexpr(.0971\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{Female}} &
  N &
  536 &
  114 &
  39 &
  44 &
  85 &
  19 &
  16 &
  48 &
  11 &
  2 &
  2 &
  916\\
 &
  \% &
  58.5\% &
  12.4\% &
  4.3\% &
  4.8\% &
  9.3\% &
  2.1\% &
  1.7\% &
  5.2\% &
  1.2\% &
  0.2\% &
  0.2\% &
  100.0\%\\
\multicolumn{1}{p{\dimexpr(.0971\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{Total}} &
  N &
  1100 &
  201 &
  86 &
  84 &
  174 &
  51 &
  32 &
  52 &
  16 &
  2 &
  2 &
  1800\\
 &
  \% &
  61.1\% &
  11.2\% &
  4.8\% &
  4.7\% &
  9.7\% &
  2.8\% &
  1.8\% &
  2.9\% &
  0.9\% &
  0.1\% &
  0.1\% &
  100.0\%\\
\tblbottomrule 
\end{tabulary}\par 
\begin{tablenotes}\footnotesize 
    
\item{Age Pearson Chi-Square- 320.222, p-0.001 Gender Pearson Chi-Square- 51.608, p-0.001}
\end{tablenotes}
\end{threeparttable}

\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {Comparison of the mean PUFA+pufa with respect to age} }
\label{tw-32a6c1ae53e7}
\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.19450000000000003\linewidth-2\tabcolsep}p{\dimexpr.1495\linewidth-2\tabcolsep}p{\dimexpr.178\linewidth-2\tabcolsep}p{\dimexpr.1405\linewidth-2\tabcolsep}p{\dimexpr.1375\linewidth-2\tabcolsep}p{\dimexpr.20\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}Age in years & N & Mean & SD & F & P\\
\tblmidrule 
4 to 6  &
  600 &
  0.98 &
  2.04 &
  \multicolumn{1}{p{\dimexpr(.1375\linewidth-2\tabcolsep)}}{\multirow{3}{\linewidth}{37.86}} &
  \multicolumn{1}{p{\dimexpr(.20\linewidth-2\tabcolsep)}}{\multirow{3}{\linewidth}{0.001}}\\
8 to 10  &
  600 &
  1.87 &
  2.21 &
   &
  \\
12 to 14  &
  600 &
  1.00 &
  1.77 &
   &
  \\
Total &
  1800 &
  1.28 &
  2.06 &
   &
  \\
\tblbottomrule 
\end{tabulary}\par 
\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {Comparison of the mean PUFA+pufa with respect to age} }
\label{tw-d7bfa605dbe7}
\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.1198\linewidth-2\tabcolsep}p{\dimexpr.13049999999999999\linewidth-2\tabcolsep}p{\dimexpr.13409999999999998\linewidth-2\tabcolsep}p{\dimexpr.15069999999999999\linewidth-2\tabcolsep}p{\dimexpr.16490000000000002\linewidth-2\tabcolsep}p{\dimexpr.14\linewidth-2\tabcolsep}p{\dimexpr.16\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}Gender & N & Mean & SD & MD & t & P\\
\tblmidrule 
Male  &
  884 &
  1.1097 &
  1.80045 &
  \multicolumn{1}{p{\dimexpr(.16490000000000002\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{0.34}} &
  \multicolumn{1}{p{\dimexpr(.14\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{-3.458}} &
  \multicolumn{1}{p{\dimexpr(.16\linewidth-2\tabcolsep)}}{\multirow{2}{\linewidth}{0.001}}\\
Female  &
  916 &
  1.4443 &
  2.26886 &
   &
   &
  \\
\tblbottomrule 
\end{tabulary}\par 
\end{table*}
\textbf{Training and calibration of examiners}

Before the actual survey, all the examiners participated in clinical calibration training workout. Following this 180 school children were inspected by each of the three examiners to assess inter-examiner reliability. The inter-examiner calibration for caries experience between 1 and 2, 2 and 3 and 1 and 3 resulted in Kappa values of 0.82 (P \textless\ 0.001), 0.88 (P \textless\ 0.001) and 0.81 (P \textless\ 0.001), respectively. Intra-examiner reproducibility was evaluated by reassessing 10\% of the samples. There was good agreement amongst the examinations by the same examiner. 

\textbf{Statistical analysis}

The data were entered in EXCEL and analysed using SPSS Version 23. Data was presented using, calculated mean values and standard deviations to express the dmft/DMFT values. Data analysis was performed using the chi-square test to find the association of the prevalence of dental caries with gender and age. 
    
\section{Results}
Distribution of study subjects according to age and sex was presented in Table~\ref{tw-f0970a3069be}. Equal number of subjects between the subject groups with the pattern of enrollment was scrutinized to reduce imbalance between the subject groups. Of 1800 children examined 49.1\% were male and 50.9\% were females.

Socio demographic variables of parents of children examined showed 61.2\% were with primary, 22.8\% were with secondary and 16\% were with higher secondary education levels. 78.8\% parents were with blue collar jobs. Evaluated socioeconomic status showed 59.7\% parents were having BPL card and classified as below poverty line and 40.3\% parents without BPL card and classified under lower middle class (Table~\ref{tw-be991301376f}).

In 8-10 years children permanent teeth caries prevalence was found to be 54.3\% which increased to 67.8 \% in12-14 years. This percentage increase of caries prevalence observed across the age was found statistically significant (p-0.001). Among 8-10 years Mean DMF was 1.71 which increased to 2.57 among 12-14 years. Permanent teeth caries prevalence among boys was 40.4\% and in girls was 41.2\%. The variation observed between the genders was found to be statistically non-significant. Among boys Mean DMF was 1.38 and among girls it was 1.47 (Tables~\ref{tw-7acd18e60597} and~\ref{tw-4004fe9d3ca2}).

Among 4-6 years children primary teeth caries prevalence was found to be 47.4\% which increased to 73.2\% in 8-10 years. This percentage increase of caries prevalence observed across the age was found statistically significant (p-0.001). Among 4-6 years Mean def was 1.30 which increased to 2.99 among 8-10 years. Primary teeth caries prevalence among boys was 42.2\% and in girls was 41\%. The variation observed between the genders was found to be statistically non-significant. Among boys Mean def was 1.38 and among girls it was 1.50 (Tables~\ref{tw-4004fe9d3ca2} and~\ref{tw-6d260d2659e4}).

Among 4-6 and 12-14 years children percentage prevalence of PUFA+ pufa was 32.2\% which increased to 52.3\% in 8-10 years. The percentage increase observed in PUFA across the age was found to be statistically significant. Prevalence of PUFA+pufa to Gender showed 36.2\% PUFA prevalence in male children and 41.5\% PUFA prevalence in female children. The difference observed in prevalence of PUFA across the gender was found to be statistically significant (Table~\ref{tw-717ece3bbaba}). 

Mean PUFA in children showed a mean of 0.98 among 4-6 years, 1.87 among 8-10 years and 1.00 in 12-14 old. This variation across the age group was found to be statistically significant (p=0.001). (Table~\ref{tw-32a6c1ae53e7}) Mean PUFA in boys was 1.10 and 1.44 in girls. The difference observed across gender was found statistically significant (p=0.001) (Table~\ref{tw-d7bfa605dbe7}). 
    
\section{Discussion}
The present study was carried out among school going children in the age group between 4{\textendash}14 years, due to ease of accessibility. It gave us a comprehensive picture of dental caries in primary, mixed, and permanent dentition. Another reason for the inclusion of such a wide age range was that caries in the primary dentition is a robust interpreter of caries in the permanent dentition and a strong indicator of the forthcoming disease. Twelve year being an important WHO index age group and also the global monitoring age for caries assessment in children was included to facilitate comparison with other published research\unskip~\citep{980677:21196359,980677:21196347,980677:21196365}.

Present study results showed 47.4\% of Primary teeth caries prevalence in 4-6 years. Study by \unskip~\citet{980677:21196365,980677:21196350,980677:21196355}  reported almost similar prevalence in their studies\unskip~\citep{980677:21196362,980677:21196377}.

\unskip~\citet{980677:21196371,980677:21196356,980677:21196349} reported a higher prevalence ranging between 65 to 90\% in their studies. 

Present study results showed among 8-10 years old children prevalence of caries increased to 73.2\%. Studies by \unskip~\citet{980677:21196368,980677:21196345,980677:21196376}  reported similar findings as ours. However studies by \unskip~\citet{980677:21196359}  and \unskip~\citet{980677:21196371}  reported a higher prevalence of 82\% in their studies. In contrary \unskip~\citet{980677:21196367}  reported a lower prevalence of 49\% among 8-10 years old children. 

Present study results showed at 4-6 and 12-14 years 32.2\% children were having one or more PUFA in oral cavity. At 8-10 years the prevalence of PUFA increased to 52.3\%. \unskip~\citet{980677:21196356} reported similar PUFA prevalence in 4-6year old children however \unskip~\citet{980677:21196360} reported 53\% in 4-6 years and 41\% I~12-14 years. \unskip~\citet{980677:21196368}  reported 47.3\% PUFA prevalence among 8-10 years. \unskip~\citet{980677:21196346,980677:21196368}  reported very less 18-19\% PUFA prevalence in their studies contrast to present study results of 32.2\%. 

Present study results showed that no statistically significant variation in the caries prevalence amongst the gender in all three age groups. \unskip~\citet{980677:21196347}  found similar results. In conflicting Vacher, \unskip~\citet{980677:21196364,980677:21196352,980677:21196365}   reported a higher caries experience among boys than in girls. Conflicting, girls were found to have higher caries prevalence by \unskip~\citet{980677:21196370,980677:21196343,980677:21196358}.

Owing to lack of awareness, motivation, accessibility, dental neglect and/or unaffordability of dental care, \unskip~\citep{980677:21196375}  present study results showed that nearly 80-90 percent of children in rural Mysore had untreated caries. The call for preventive care was found to be higher after 9yrs of age and it increased as the age advanced.

Hence we recommend for the given population a constant monitoring for prevalence of oral diseases, at regular intervals to recognize the burden and spread of the disease, and ascertain the need of preventive and restorative care to help the population to be disease free \unskip~\citep{980677:21196354}.

As most of the parents of rural school children were employed as daily wage workers or migrant laborers and may not find time to visit the dentist as that would lead to loss of a day's earnings, on site fluoride and pit fissure sealant application program can be initiated by utilizing the services of mobile dental van for the specific protection of high-risk patients. Suggested systematic oral health promotion program for Rural India is, recruiting teachers as initial information propagators for educating and motivating the school going children as teachers have a great impact on their developing minds and act as role models. Oral health professionals can train and up-skill the Teachers for early detection of oral diseases helping to initiate rapid intervention by early referral\unskip~\citep{980677:21196344}.  Also the putting into practice of community-based oral health program is a matter of urgency. One such program could be commenced through oral health promoting school schemes in Rural Mysore while identifying the significant caries risk factors in children.
    
\section{Conclusion}
In 8-10 years children permanent teeth caries prevalence was found to be 54.3\% which increased to 67.8 \% in12-14 years. Among 4-6 years children primary teeth caries prevalence was found to be 47.4\% which increased to 73.2\% in 8-10 years. Among 4-6 and 12-14 years children percentage prevalence of PUFA was 32.2\% which increased to 52.3\% in 8-10 years. No clinically significant variation was observed in Caries prevalence among gender.\textbf{\space }
\section*{Acknowledgement}We would like to acknowledge the Government school authorities of rural Mysuru for valuable cooperation.

\textbf{Conflict of interest}

The authors declare that they have no conflict of interest for this study.

\textbf{Funding support}

The authors declare that they have no funding support for this study.
    

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