Genoprotective effect of D-Pinitol isolated from aerial parts of Soybean plants against Doxorubicin-induced genotoxicity evaluated by in vitro comet assay in Vero cell lines
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Genotoxicity, Doxorubicin, D-Pinitol, In vitro Comet assay
Abstract
Doxorubicin is a chemotherapeutic agent with a genotoxic effect on normal cells at its therapeutic dose itself. D-Pinitol is an abundantly available carbohydrate in Soybean plants and has been proven for antioxidant and anti-inflammatory activities. Our investigation was examined by in vitro comet assay to explore the genoprotective effect of D-Pinitol in normal cells against Doxorubicin-induced genotoxicity in Vero cell lines. In vitro comet assay treatment groups were Vero Cell lines with culture medium (control group), Doxorubicin (0.15μg/ml), D-Pinitol (0.05×103 mM, 0.125×103 mM, and 0.25×103 mM) alone, and pretreatment with D-Pinitol (0.05×103 mM, 0.125×103 mM, and 0.25×103 mM) before Doxorubicin (0.15μg/ml) treatment. When compared to the control group, D-Pinitol alone treated groups showed no significant changes in the percentage of DNA damage. For the evaluation of the genoprotective effect of D-Pinitol, the % DNA damage in the D-Pinitol, and Doxorubicin simultaneously treated groups were compared to the Doxorubicin alone treated group. The results showed that Doxorubicin-induced genotoxic effect in Vero cell lines was significantly reduced by D-Pinitol in a dose-dependent manner by reducing DNA damage. Our findings confirmed that D-Pinitol had no genotoxic effect and it showed a genoprotective effect against Doxorubicin-induced genotoxicity.
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References
Al-Ashaal, H. A., Fahmy, M. A., Melek, F. R., Aly, N. H., Hasan, Z. M. 2012. Effect of supplemented soybean (Glycine max L) diet and extracts on aluminum sulfate-induced genotoxicity. Toxicological & Environmental Chemistry, 94(5):965–986. ISSN 0277-2248,1029-0486.
Al-Shdefat, R. I., Abd-ElAziz, M. A., Al-Saikhan, F. I. 2014. Genoprotective and Genotoxic Effects of Thymoquinone on Doxorubicin-Induced Damage in Isolated Human Leukocytes. Tropical Journal of Pharmaceutical Research, 13(12):2015–2020. ISSN 1596-5996, 1596-9827.
Anderson, D., Yu, T., Phillips, B. J., Schmezer, P. 1994. The effect of various antioxidants and other modifying agents on oxygen-radical-generated DNA damage in human lymphocytes in the Comet assay. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 307:261–271. ISSN 0027-5107.
Arts, I. C., Hollman, P. C. 2005. Polyphenols and disease risk in epidemiologic studies. The American Journal of Clinical Nutrition, 81(1):317S–325S. ISSN 0002-9165, 1938-3207.
Choi, M. S., Lee, M. K., Jung, U. J., Kim, H. J., Do, G. M., Park, Y. B., Jeon, S. M. 2009. Metabolic response of soy pinitol on lipid-lowering, antioxidant and hepatoprotective action in hamsters fed-high fat and high cholesterol diet. Molecular Nutrition & Food Research, 53(6):751–759.
Hajra, S., Patra, A. R., Basu, A., Bhattacharya, S. 2018. Prevention of doxorubicin (DOX)-induced genotoxicity and cardiotoxicity: Effect of plant derived small molecule indole-3-carbinol (I3C) on oxidative stress and inflammation. Biomedicine & Pharmacotherapy, 101:228–243. ISSN 0753-3322.
Kim, J. C., Shin, J. Y., Shin, D. H., Kim, Y. B., Park, S. C., Park, R. D., Shin, Y. C. 2005a. Synergistic anti-inflammatory effects of pinitol and glucosamine in rats. Phytotherapy Research, 19(12):1048–1051.
Kim, J. I., Kim, J. C., Kang, M. J., Lee, M. S., Kim, J. J., Cha, I. J. 2005b. Effects of pinitol isolated from soybeans on glycaemic control and cardiovascular risk factors in Korean patients with type II diabetes mellitus: a randomized controlled study. European Journal of Clinical Nutrition, 59(3):456–458. ISSN 0954-3007, 1476-5640.
Nandhakumar, S., Parasuraman, S., Shanmugam, M., Rao, K. R., Chand, P., Bhat, B. V. 2011. Evaluation of DNA damage using single-cell gel electrophoresis (Comet Assay). Journal of Pharmacology and Pharmacotherapeutics, 2(2):107–111. ISSN 0976-500X.
Orthen, B., Popp, M., Smirnoff, N. 1994. Hydroxyl radical scavenging properties of cyclitols. Royal Society of Edinburgh, 102:269–272. ISSN 0269-7270, 2053-5910.
Quiles, J. L., Huertas, J. R., Battino, M., Mataix, J., Ramírez-Tortosa, M. C. 2002. Antioxidant nutrients and adriamycin toxicity. Toxicology, 180(1):79–95. ISSN 0300-483X.
Remesh, A. 2012. Toxicities of anticancer drugs and its management. International Journal of Basic & Clinical Pharmacology, 1(1):2–12. ISSN 2319-2003.
Rengarajan, T., Rajendran, P., Nandakumar, N., Balasubramanian, M. P., Nishigaki, I., Haribabu, L. 2014. Free radical scavenging and antioxidant activity of D-Pinitol against 7, 12 dimethylbenz (a) anthracene induced breast cancer in sprague dawley rats. Asian Pacific Journal of Tropical Disease, 4(5):384–390. ISSN 2222-1808.
Singh, N.P., McCoy, M.T., Tice, R.R., Schneider, E.L. 1988. A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research, 175(1):184–191. ISSN 0014-4827.
Singh, R. K., Pandey, B. L., Tripathi, M., Pandey, V. B. 2001. Anti-inflammatory effect of (+)-Pinitol. Fitoterapia, 72(2):168–170. ISSN 0367-326X.
Sivakumar, S., Palsamy, P., Subramanian, S. P. 2010. Attenuation of oxidative stress and alteration of hepatic tissue ultrastructure by D-Pinitol in streptozotocin-induced diabetic rats. Free Radical Research, 44(6):668–678. ISSN 1071-5762, 1029-2470.
Sripathi, S. K., Poongothai, G. 2013. A review on Insulinomimetic Pinitol from plants. International Journal of Pharma and Bio Sciences, 4(2):992– 1009.
Streeter, J. G. 2001. Simple partial purification of D-Pinitol from soybean leaves. Crop Science, 41(6):1985–1987. ISSN 0011-183X.
Tice, R. R., Agurell, E., Anderson, D., Burlinson, B., Hartmann, A., Kobayashi, H., Miyamae, Y., Rojas, E., Ryu, J. C., Sasaki, Y. F. 2000. Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing. Environmental and Molecular Mutagenesis, 35(3):206–221. ISSN 0893-6692.
Čabarkapa, A., Živković, L., Žukovec, D., Djelić, Bajić, V., Dekanski, D., Spremo-Potparević, B. 2014. Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes. Toxicology in Vitro, 28(3):451–456. ISSN 0887-2333.
Visvardis, E.E., Haveles, K.S., Pataryas, T.A., Margaritis, L.H., Sophianopoulou, V., Sideris, E.G. 2000. Diversity of peripheral blood mononuclear cells as revealed by a novel multiple microgel “Comet assay”. Environmental and Molecular Mutagenesis, 36:32–39. ISSN 0893-6692,1098-2280.
Wang, L., Chen, Q., Qi, H., Wang, C., Wang, C., Zhang, J., Dong, L. 2016. Doxorubicin-Induced Systemic Inflammation Is Driven by Upregulation of Toll-Like Receptor TLR4 and Endotoxin Leakage. Cancer Research, 76(22):6631–6642.
Xu, Z., Lin, S., Wu, W., Tan, H., Wang, Z., Cheng, C., Lu, L., Zhang, X. 2008. Ghrelin prevents Doxorubicin induced cardiotoxicity through TNF-alpha/NF-κB pathways and mitochondrial protective mechanisms. Toxicology, 247(2-3):133–138.
Zhou, Y., Park, C. M., Cho, C. W., Song, Y. S. 2008. Protective Effect of Pinitol against D-Galactosamine Induced Hepatotoxicity in Rats Fed on a High-Fat Diet. Bioscience, Biotechnology, and Biochemistry, 72(7):1657–1666.
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