\documentclass[twocolumn,twoside]{article}
\makeatletter\if@twocolumn\PassOptionsToPackage{switch}{lineno}\else\fi\makeatother

\usepackage{amsfonts,amssymb,amsbsy,latexsym,amsmath,tabulary,graphicx,times,xcolor}

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
% Following additional macros are required to function some 
% functions which are not available in the class used.
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
\usepackage{url,multirow,morefloats,floatflt,cancel,tfrupee}
\makeatletter


\AtBeginDocument{\@ifpackageloaded{textcomp}{}{\usepackage{textcomp}}}
\makeatother
\usepackage{colortbl}
\usepackage{xcolor}
\usepackage{pifont}
\usepackage[nointegrals]{wasysym}
\urlstyle{rm}
\makeatletter

%%%For Table column width calculation.
\def\mcWidth#1{\csname TY@F#1\endcsname+\tabcolsep}

%%Hacking center and right align for table
\def\cAlignHack{\rightskip\@flushglue\leftskip\@flushglue\parindent\z@\parfillskip\z@skip}
\def\rAlignHack{\rightskip\z@skip\leftskip\@flushglue \parindent\z@\parfillskip\z@skip}

%Etal definition in references
\@ifundefined{etal}{\def\etal{\textit{et~al}}}{}


%\if@twocolumn\usepackage{dblfloatfix}\fi
\usepackage{ifxetex}
\ifxetex\else\if@twocolumn\@ifpackageloaded{stfloats}{}{\usepackage{dblfloatfix}}\fi\fi

\AtBeginDocument{
\expandafter\ifx\csname eqalign\endcsname\relax
\def\eqalign#1{\null\vcenter{\def\\{\cr}\openup\jot\m@th
  \ialign{\strut$\displaystyle{##}$\hfil&$\displaystyle{{}##}$\hfil
      \crcr#1\crcr}}\,}
\fi
}

%For fixing hardfail when unicode letters appear inside table with endfloat
\AtBeginDocument{%
  \@ifpackageloaded{endfloat}%
   {\renewcommand\efloat@iwrite[1]{\immediate\expandafter\protected@write\csname efloat@post#1\endcsname{}}}{\newif\ifefloat@tables}%
}%

\def\BreakURLText#1{\@tfor\brk@tempa:=#1\do{\brk@tempa\hskip0pt}}
\let\lt=<
\let\gt=>
\def\processVert{\ifmmode|\else\textbar\fi}
\let\processvert\processVert

\@ifundefined{subparagraph}{
\def\subparagraph{\@startsection{paragraph}{5}{2\parindent}{0ex plus 0.1ex minus 0.1ex}%
{0ex}{\normalfont\small\itshape}}%
}{}

% These are now gobbled, so won't appear in the PDF.
\newcommand\role[1]{\unskip}
\newcommand\aucollab[1]{\unskip}
  
\@ifundefined{tsGraphicsScaleX}{\gdef\tsGraphicsScaleX{1}}{}
\@ifundefined{tsGraphicsScaleY}{\gdef\tsGraphicsScaleY{.9}}{}
% To automatically resize figures to fit inside the text area
\def\checkGraphicsWidth{\ifdim\Gin@nat@width>\linewidth
	\tsGraphicsScaleX\linewidth\else\Gin@nat@width\fi}

\def\checkGraphicsHeight{\ifdim\Gin@nat@height>.9\textheight
	\tsGraphicsScaleY\textheight\else\Gin@nat@height\fi}

\def\fixFloatSize#1{}%\@ifundefined{processdelayedfloats}{\setbox0=\hbox{\includegraphics{#1}}\ifnum\wd0<\columnwidth\relax\renewenvironment{figure*}{\begin{figure}}{\end{figure}}\fi}{}}
\let\ts@includegraphics\includegraphics

\def\inlinegraphic[#1]#2{{\edef\@tempa{#1}\edef\baseline@shift{\ifx\@tempa\@empty0\else#1\fi}\edef\tempZ{\the\numexpr(\numexpr(\baseline@shift*\f@size/100))}\protect\raisebox{\tempZ pt}{\ts@includegraphics{#2}}}}

%\renewcommand{\includegraphics}[1]{\ts@includegraphics[width=\checkGraphicsWidth]{#1}}
\AtBeginDocument{\def\includegraphics{\@ifnextchar[{\ts@includegraphics}{\ts@includegraphics[width=\checkGraphicsWidth,height=\checkGraphicsHeight,keepaspectratio]}}}

\DeclareMathAlphabet{\mathpzc}{OT1}{pzc}{m}{it}

\def\URL#1#2{\@ifundefined{href}{#2}{\href{#1}{#2}}}

%%For url break
\def\UrlOrds{\do\*\do\-\do\~\do\'\do\"\do\-}%
\g@addto@macro{\UrlBreaks}{\UrlOrds}



\edef\fntEncoding{\f@encoding}
\def\EUoneEnc{EU1}
\makeatother
\def\floatpagefraction{0.8} 
\def\dblfloatpagefraction{0.8}
\def\style#1#2{#2}
\def\xxxguillemotleft{\fontencoding{T1}\selectfont\guillemotleft}
\def\xxxguillemotright{\fontencoding{T1}\selectfont\guillemotright}

\newif\ifmultipleabstract\multipleabstractfalse%
\newenvironment{typesetAbstractGroup}{}{}%

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%

\usepackage[authoryear]{natbib}



\newif\ifnokeywords\nokeywordsfalse

\makeatletter\input{size10-pointfive.clo}\makeatother%
\definecolor{kwdboxcolor}{RGB}{242,242,242}
\usepackage[hidelinks,colorlinks=true,allcolors=blue]{hyperref}
\linespread{1}                 
\def\floatpagefraction{0.8}

\usepackage[paperheight=11.69in,paperwidth=8.26in,top=1in,bottom=1in,left=1in,right=.75in,headsep=24pt]{geometry}

\usepackage{multirow-custom}

\makeatletter

\def\hlinewd#1{%
  \noalign{\ifnum0=`}\fi\hrule \@height #1%
  \futurelet\reserved@a\@xhline}
\def\tbltoprule{\hlinewd{1pt}\\[-14pt]}
\def\tblbottomrule{\noalign{\vspace*{6pt}}\hline\noalign{\vspace*{2pt}}}
\def\tblmidrule{\hline\noalign{\vspace*{2pt}}}



\let\@articleType\@empty
\let\@journalDoi\@empty
\let\@journalVolume\@empty
\let\@journalIssue\@empty
\let\@crossMarkLink\@empty
\let\@receivedDate\@empty
\let\@acceptedDate\@empty
\let\@revisedDate\@empty
\let\@publishedDate\@empty
\let\@copyrightYear\@empty
\let\@firstPage\@empty
\def\articleType#1{\gdef\@articleType{#1}}
\def\journalDoi#1{\gdef\@journalDoi{#1}}
\def\crossMarkLink#1{\gdef\@crossMarkLink{#1}}
\def\receivedDate#1{\gdef\@receivedDate{#1}}
\def\acceptedDate#1{\gdef\@acceptedDate{#1}}
\def\revisedDate#1{\gdef\@revisedDate{#1}}
\def\publishedDate#1{\gdef\@publishedDate{#1}}
\def\copyrightYear#1{\gdef\@copyrightYear{#1}}
\def\journalVolume#1{\gdef\@journalVolume{#1}}
\def\journalIssue#1{\gdef\@journalIssue{#1}}
\def\firstPage#1{\gdef\@firstPage{#1}}
\let\@issuelogo\@empty%
\let\@issuelogoex\@empty%
\def\issuelogo#1{\gdef\@issuelogo{#1}}
\def\issuelogoex#1{\gdef\@issuelogoex{#1}}


\def\author#1{%
  \gdef\@author{%
    \hskip-\dimexpr(\tabcolsep)\hskip5pt%
    \parbox{\dimexpr\textwidth-1pt}%
    {\fontsize{11}{13}\selectfont\raggedright #1}%
  }%
}


\usepackage{pharmascope-abs}
\usepackage{caption}
\usepackage{lastpage}
\usepackage{fancyhdr}
\usepackage[noindentafter,explicit]{titlesec}
\usepackage{fontspec}

\setmainfont[%
BoldFont=cambriab.ttf,%
ItalicFont=CAMBRIAI.TTF,%
BoldItalicFont=CAMBRIAZ.TTF]{Cambria.ttf}
\lefthyphenmin = 3

\def\title#1{%
  \gdef\@title{%
      \vspace*{-40pt}%
       \ifx\@articleType\@empty\else{\fontsize{10}{12}\scshape\selectfont\hspace{8pt}\@articleType\hfill\mbox{}\par\vspace{2pt}}\fi%
       \minipage{\linewidth}
         \hrulefill\\[-0.7pt]%%{\includegraphics[width=75pt,height=50pt]{ijrps_logo.png}}\hfill
         \mbox{~}\hspace{5pt}\parbox{.1\linewidth}{\IfFileExists{images/\@issuelogo.\@issuelogoex}{\includegraphics[width=79pt,height=61pt]{images/\@issuelogo.\@issuelogoex}}{\includegraphics[width=75pt,height=50pt]{ijrps_logo.png}}}\hfill
         \fcolorbox{kwdboxcolor}{kwdboxcolor}{\parbox{.792\linewidth}{%
          \begin{center}\fontsize{17}{17}\selectfont\scshape\vskip-7pt International Journal of Research in Pharmaceutical Sciences\hfill\end{center}%
          \vspace*{-10pt}\hspace*{4pt}{\fontsize{8}{9}\selectfont Published by JK Welfare \& Pharmascope Foundation\hfill  Journal Home Page: \href{www.ijrps.com}{\color{blue}\underline{\smash{www.ijrps.com}}}}\hspace*{4pt}\mbox{}}}%
       \par\vspace*{-1pt}\rule{\linewidth}{1.3pt}%
       \endminipage%
    \par\vspace*{9.2pt}\parbox{.98\linewidth}{\linespread{.9}\raggedright\fontsize{14}{17}\selectfont #1}%
    \vspace*{-8pt}%
  }
}


\setlength{\parindent}{0pt}
\setlength{\parskip}{0.4pc plus 1pt minus 1pt}

\def\abbrvJournalTitle{Int. J. Res. Pharm. Sci.}

\fancypagestyle{headings}{%
  \renewcommand{\headrulewidth}{0pt}%
  \renewcommand{\footrulewidth}{0.3pt}
  \fancyhf{}%
  \fancyhead[R]{%
    \fontsize{9.12}{11}\selectfont\RunningAuthor,\ \abbrvJournalTitle,\ \ifx\@journalVolume\@empty X\else\@journalVolume\fi%
    \ifx\@journalIssue\@empty\else(\@journalIssue)\fi%
    ,\ \ifx\@firstPage\@empty 1\else\@firstPage\fi-\pageref*{LastPage}%
  }%
  \fancyfoot[LO,RE]{\fontsize{9.12}{11}\selectfont\textcopyright\ International Journal of Research in Pharmaceutical Sciences}%
  \fancyfoot[RO,LE]{\fontsize{9.12}{11}\selectfont\thepage}
}\pagestyle{headings}

                                                         
\fancypagestyle{plain}{%
  \renewcommand{\headrulewidth}{0pt}%
  \renewcommand{\footrulewidth}{0.3pt}%
  \fancyhf{}%
  \fancyhead[R]{%
    \fontsize{9.12}{11}\selectfont\RunningAuthor,\ \abbrvJournalTitle,\ \ifx\@journalVolume\@empty X\else\@journalVolume\fi%
    \ifx\@journalIssue\@empty\else(\@journalIssue)\fi%
    ,\ \ifx\@firstPage\@empty 1\else\@firstPage\fi-\pageref*{LastPage}%
  }%
  \fancyfoot[LO,RE]{\fontsize{9.12}{11}\selectfont\textcopyright\ International Journal of Research in Pharmaceutical Sciences}%
  \fancyfoot[RO,LE]{\fontsize{9.12}{11}\selectfont\thepage}
  \ifx\@firstPage\@empty\else\setcounter{page}{\@firstPage}\fi
}


\def\NormalBaseline{\def\baselinestretch{1.1}}
\usepackage{textcase}
\setcounter{secnumdepth}{0}

\titleformat{\section}[block]{\bfseries\boldmath\NormalBaseline\filright\fontsize{10.5}{13}\selectfont}
{\thesection}
{6pt}
{\MakeTextUppercase{#1}}
[]
\titleformat{\subsection}[block]{\bfseries\boldmath\NormalBaseline\filright\fontsize{10.5}{12}\selectfont}
{\thesubsection}
{6pt}
{#1}
[]
\titleformat{\subsubsection}[block]{\NormalBaseline\filright\fontsize{10.5}{12}\selectfont}
{\thesubsubsection}
{6pt}
{#1}
[]
\titleformat{\paragraph}[block]{\NormalBaseline\filright\fontsize{10.5}{10}\selectfont}
{\theparagraph}
{6pt}
{#1}
[]
\titleformat{\subparagraph}[block]{\NormalBaseline\filright\fontsize{10.5}{12}\selectfont}
{\thesubparagraph}
{6pt}
{#1}
[]

\titlespacing{\section}{0pt}{.5\baselineskip}{.5\baselineskip}  
\titlespacing{\subsection}{0pt}{.5\baselineskip}{.5\baselineskip}  
\titlespacing{\subsubsection}{0pt}{.5\baselineskip}{.5\baselineskip}  
\titlespacing{\paragraph}{0pt}{.5\baselineskip}{.5\baselineskip}  
\titlespacing{\subparagraph}{0pt}{.5\baselineskip}{.5\baselineskip}

\captionsetup[figure]{skip=1.4pt,font=bf,labelsep=colon,justification=raggedright,singlelinecheck=false}
\captionsetup[table]{skip=1.4pt,font=bf,labelsep=colon,justification=raggedright,singlelinecheck=false}

\def\bibyear#1{#1}
%\def\bibjtitle#1{#1}   %%Publisher request
\def\bibauand{}
\setlength\bibsep{3pt}
\setlength\bibhang{8pt}

\makeatother
\date{}


\usepackage{float}

\begin{document}


\def\RunningAuthor{Smitha M et al.}
\firstPage{292}
\articleType{Original Article}
\receivedDate{11 Nov 2020}
\acceptedDate{19 Dec 2020}
\revisedDate{16 Dec 2020}
\journalVolume{2020, 11 (SPL4)}
\journalDoi{ijrps.v11iSPL4.3787}
\copyrightYear{2020}
\def\authorCount{4}
\def\affCount{2}

\def\journalTitle{International Journal of Research in Pharmaceutical Sciences}

\title{\textbf{Analysis on Bulk Pharmaceutical Formulation, Validation and Estimation Using Levetiracetam Methods}}
\author{Smitha~M\textsuperscript{*}\textsuperscript{1},  
            Kal~Satyavathi~Devi\textsuperscript{1},  
            Virendra~Kumar\textsuperscript{2}, 
            Jaffar~Hussain\textsuperscript{2}~\\[5pt]{\textsuperscript{1}Department of OBG, Konaseema Institute of Medical Sciences Research Foundation, Amalapuram, Andhra Pradesh- 533201, India}~\\{\textsuperscript{2}Department of Forensic Medicine, Meenakshi Academy of Higher Education and Research, Chennai - 600078, Tamilnadu, India}}

\begin{abstract}
A new sensitive, specific, direct, exact and correct RP-HPLC technique is established and authenticated to estimate Levetiracetam in Majority and Pharmaceutical Tablet Formulations. An isocratic, turned around period HPLC system might have been created should differentiate the pill starting with the corruption products, Phenomenex Gemini 5\ensuremath{\mu} C18 (2) 100A (250 x 4.60mm, 5 \ensuremath{\mu}) section. Hamilton syringe (705 NR, 50 \ensuremath{\mu}L) might have been utilized to injecting example Furthermore standard result. The versatile stage comprises about mixture of Methanol: Acetonitrile in the proportion (90:10 v/v) toward A stream rate about 1.0 ml /min. UV identification might have been performed toward 210 nm. The linearity might have been made for Levetiracetam in the extent from claiming 5- 30\ensuremath{\mu}g/ml for relationship coefficient about 0.9997. LOD Also LOQ were found will make 0.076\ensuremath{\mu}g/ml Furthermore 0.23\ensuremath{\mu}g/ml individually. Maintenance duration of the time of Levetiracetam were found with make 2.281min and 2.274min. \% recuperation might have been discovered on be 99.78-100.45 What's more \%RSD might have been found for over \ensuremath{\pm}2. Those system needs been approved as stated by ICH rules to linearity, precision, accuracy, robustness, ruggedness, LOD furthermore LOQ. Those produced approved system might have been effectively connected to dependable quantification about Levetiracetam to mass and pharmaceutical measurement type.
\end{abstract}\def\keywordstitle{Keywords}

\begin{keywords}Levetiracetam,\newline RP- HPLC,\newline Authentication,\newline Pharmaceutical Formulations
\end{keywords}
\twocolumn[ \maketitle {\printKwdAbsBox}]
\makeatletter{\raggedright\textsuperscript{*}Corresponding Author\par Name:\ Smitha~M~\\ Phone:\ 8106141143~\\  Email:\ smitha43@gmail.com
        \par}\vspace*{-11pt}\hrulefill\par{\fontsize{12}{14}\selectfont ISSN: 0975-7538}\par% 
        \textsc{DOI:}\ \href{https://doi.org/10.26452/\@journalDoi}{\textcolor{blue}{\underline{\smash{https://doi.org/10.26452/\@journalDoi}}}}\par%
        \vspace*{-11pt}\hrulefill\\{\fontsize{9.12}{10.12}\selectfont Production and Hosted by}\par{IJRPS |~\href{www.ijrps.com}{www.ijrps.com}}\par%
        \vspace*{-2pt}{\fontsize{9.12}{10.12}\selectfont\textcopyright\ \@copyrightYear\ $|$ All rights reserved.}\par%
        \vspace*{-11pt}\rule{\linewidth}{1.2pt}
        \makeatother
        
    
\section{Introduction}
Levetiracetam is an anticonvulsant drug used treat epilepsy \unskip~\citep{963646:21047374}. Levetiracetam is a drug within the pyrrolidine session is utilized to delicacy numerous types of appropriations \unskip~\citep{963646:21047376}. Chemically it is known as pyrrolidinone and acetamide derivatives. Levetiracetam may selectively prevent synchronization of epileptic form bust firing and propagation of seizure activity. It is correspondingly utilized to treat neuropathic pain \unskip~\citep{963646:21047373}. The chemical term of Levetiracetam is (S)-2-(2-oxopyrollidin-1- yl) butanamide with molecular weight of 170.20g/ml Figure~\ref{f-c81028d165b3}. Fiction survey exposed that there were limited investigational approaches to be described for the purpose of the Levetiracetam in pure drug and pharmaceutical dosage form by using UV spectrophotometric,\ensuremath{^{}} HPLC, \unskip~\citep{963646:21047381,963646:21047378}. The purpose of the current work is to progress and validate a novel, fast, exact and specific area under curve UV spectrophotometric technique for approximation of Levetiracetam in bulk and tablet dosage form.
\bgroup
\fixFloatSize{images/a118722a-df86-463e-909a-c54efce49521-upicture1.png}
\begin{figure}[!htbp]
\centering \makeatletter\IfFileExists{images/a118722a-df86-463e-909a-c54efce49521-upicture1.png}{\includegraphics{images/a118722a-df86-463e-909a-c54efce49521-upicture1.png}}{}
\makeatother 
\caption{\boldmath {Chemical structure of levetiracetam.}}
\label{f-c81028d165b3}
\end{figure}
\egroup

    
\section{Materials and Methods}
\textbf{Material and  reagents}

The Levetiracetam was obtained as a gift sample from the pharmaceutical industry and Levipiltablet obtained from  Pharmacy store. Methanol and  distilled water were obtained Bharathi  College of pharmacy, Bharathi nagara, KM Doddi, Maddur Taluk, Mandya District, India. All  chemicals used are of HPLC grade. Distilled water is utilized during the research \unskip~\citep{963646:21047379}.


\begin{table}[!htbp]
\caption{\boldmath {HPLC method development parameter.} }
\label{tw-8ccb7fca5c1a}
\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.4876\linewidth-2\tabcolsep}p{\dimexpr.5124\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}\multicolumn{2}{p{\dimexpr(1\linewidth-2\tabcolsep)}}{\cAlignHack HPLC method development parameters}\\
\tblmidrule 
Column &
  C18, 250 X 4.6 mm, 5\ensuremath{\mu}\\
Flow rate &
  1.0 mL / min\\
Wavelength &
  210 nm\\
Column temperature &
  30\ensuremath{^\circ}C\\
Injection volume &
  50 \ensuremath{\mu}L\\
Run time &
  7 minutes\\
Diluents &
  Mobile phase\\
Elution &
  Isocratic\\
\tblbottomrule 
\end{tabulary}\par 
\end{table}
\textbf{Instrumentation}

Chromatographic separation might have been performed once a Shimadzu LC-20AT HPLC framework including a variable wavelength programmable UV/ VIS identifier SPD-20A (VP series), Shimadzu LC-20AT (VP series) pump furthermore phenomenex gemini 5\ensuremath{\mu} C18 (2) 100A (250 x 4. 60mm, 5 \ensuremath{\mu}) section Table~\ref{tw-8ccb7fca5c1a}. Hamilton syringe (705 NR, 50 \ensuremath{\mu}L) might have been utilized for injecting test also standard result. Information might have been aggregated utilizing Spinchrom programming. Research of solutions mobile stage preparation. The mobile stage contained a combination of methanol (90\%), acetonitrile (10\%) in the ratio of v/v, that is clean concluded a film and vented earlier use. pH adjusted to 3.5 with 0.1\% Ortho-phosphoric acid.

\textbf{Preparation of sample Standard Solution}

The formulation tablets of Levetiracetam (Levipil- 500mg) were crushed to give finely powdered material. From the powder prepared a 100 mg of Levetiracetam was accurately weighed, transferred in a 100 ml volumetric flask, add 30 ml of diluents and sonicate to dissolve and dilute to volume with diluent. 

Transfer 10 ml of the standard stock solution into 100 ml volumetric flask and dilute to volume with diluent \unskip~\citep{963646:21047377}. And an appropriate concentration of sample was prepared at the time of analysis. 50\ensuremath{\mu }l of these solutions were injected in triplicate into HPLC system and the peak areas are verified.


\bgroup
\fixFloatSize{images/b9de0042-f002-4676-b541-f1d7be39d28b-upicture2.png}
\begin{figure}[!htbp]
\centering \makeatletter\IfFileExists{images/b9de0042-f002-4676-b541-f1d7be39d28b-upicture2.png}{\includegraphics{images/b9de0042-f002-4676-b541-f1d7be39d28b-upicture2.png}}{}
\makeatother 
\caption{\boldmath {Chromatogram of standard solution of Levetiracetam.}}
\label{f-5503b738fe7a}
\end{figure}
\egroup

\begin{table}[!htbp]
\caption{\boldmath {Specificity of Levetiracetam.} }
\label{tw-9015f28fba38}
\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.5685\linewidth-2\tabcolsep}p{\dimexpr.4315\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}Name of the solution & \cAlignHack Retention time in min\\
\tblmidrule 
Blank &
  \cAlignHack 0\\
Levetiracetam(Standard) &
  \cAlignHack 2.274\\
Levetiracetam(Sample) &
  \cAlignHack 2.281\\
\tblbottomrule 
\end{tabulary}\par 
\end{table}
\textbf{Preparation of Standard solution}

Levetiracetam weigh and transmission the tablet powder equal to 100 mg of Levetiracetam into 100 ml volumetric flask add 30 ml of dilutant, sonicate to melt for 10 proceedings and diluted to capacity by dilutant. Additional strainer the resolution finished 0.45\ensuremath{\mu} filter and an appropriate concentration of sample is equipped at the period of examination. 50\ensuremath{\mu }l of these solutions were injected in triplicate into HPLC system and preceded as said for the standard respectively.


\begin{table*}[!htbp]
\caption{\boldmath {Linearity of Levetiracetam.} }
\label{tw-429b89cd0d3e}
\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.359\linewidth-2\tabcolsep}p{\dimexpr.33830000000000005\linewidth-2\tabcolsep}p{\dimexpr.3027\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}\cAlignHack Concentration (\ensuremath{\mu }g/ml) & \cAlignHack Retention time (min) & \cAlignHack Peak area* (mv)\\
\tblmidrule 
\cAlignHack 5 &
  \cAlignHack 2.281 &
  \cAlignHack 25912\\
\cAlignHack 10 &
  \cAlignHack 2.283 &
  \cAlignHack 52996\\
\cAlignHack 15 &
  \cAlignHack 2.279 &
  \cAlignHack 78486\\
\cAlignHack 20 &
  \cAlignHack 2.281 &
  \cAlignHack 103841\\
\cAlignHack 25 &
  \cAlignHack 2.280 &
  \cAlignHack 128401\\
\cAlignHack 30 &
  \cAlignHack 2.279 &
  \cAlignHack 157356\\
\tblbottomrule 
\end{tabulary}\par 
\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {Results of recovery of Levetiracetam.} }
\label{tw-d50ec13e36ea}
\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.2232\linewidth-2\tabcolsep}p{\dimexpr.21\linewidth-2\tabcolsep}p{\dimexpr.16680000000000003\linewidth-2\tabcolsep}p{\dimexpr.2953\linewidth-2\tabcolsep}p{\dimexpr.1047\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}\cAlignHack Level of Addition/\% & \cAlignHack Amount added (\ensuremath{\mu }g/ml) & \cAlignHack Amount found & \cAlignHack \%Recovery \ensuremath{\pm} Standard deviation* & \cAlignHack \%RSD\\
\tblmidrule 
\cAlignHack  &
  \cAlignHack  &
  \cAlignHack 30.10 &
  \cAlignHack  &
  \cAlignHack \\
\cAlignHack 50 &
  \cAlignHack 10 &
  \cAlignHack 29.91 &
  \cAlignHack 99.78\ensuremath{\pm}0.704 &
  \cAlignHack 0.705\\
 &
  \cAlignHack  &
  \cAlignHack 29.70 &
  \cAlignHack  &
  \cAlignHack \\
\cAlignHack  &
  \cAlignHack  &
  \cAlignHack 39.89 &
  \cAlignHack  &
  \cAlignHack \\
\cAlignHack 100 &
  \cAlignHack 20 &
  \cAlignHack 40.42 &
  \cAlignHack 100.45\ensuremath{\pm}0.676 &
  \cAlignHack 0.666\\
 &
  \cAlignHack  &
  \cAlignHack 40.24 &
  \cAlignHack  &
  \cAlignHack \\
\cAlignHack  &
  \cAlignHack  &
  \cAlignHack 49.97 &
  \cAlignHack  &
  \cAlignHack \\
\cAlignHack 150 &
  \cAlignHack 30 &
  \cAlignHack 50.05 &
  \cAlignHack 99.65\ensuremath{\pm}0.567 &
  \cAlignHack 0.568\\
 &
  \cAlignHack  &
  \cAlignHack 49.5 &
  \cAlignHack  &
  \cAlignHack \\
\tblbottomrule 
\end{tabulary}\par 
\end{table*}

    
\section{Results and Discussion}
\textbf{Authentication of the projected technique}

The projected techniques authenticated as per ICH strategies \unskip~\citep{963646:21047382}. The strictures concentrated on to acceptance were specificity, linearity, precision, accuracy, robustness, framework suitability, breaking point of detection, cutoff of quantification and also result solidness in Figure~\ref{f-5503b738fe7a}.

\textbf{Specificity}

Toward thinking about those chromatograms from claiming blank, standard also example (Prepared from Formulation). It might have been found that there is no obstruction because of excipients in the tablet detailing. Furthermore, additionally discovered beneficial correspondence the middle of those maintenance times of standard What's more example \unskip~\citep{963646:21047372}. The specificity effects are indicated in Table~\ref{tw-9015f28fba38}.

\textbf{Linearity}

The linearity of the reaction of the medication regardless might have been checked during six focus levels, extending starting with 5-30\ensuremath{\mu }g/ ml about Levetiracetam Previously, every linearity level were arranged. 50\ensuremath{\mu}l from claiming each centralization might have been injected in copy under those HPLC framework. This reaction might have been perused during 210 nm and the comparing chromatograms were recorded \unskip~\citep{963646:21047375}. Starting with these chromatograms, the mean crest ranges were presented in Table~\ref{tw-429b89cd0d3e}.


\begin{table}[!htbp]
\caption{\boldmath {Ruggedness of Levetiracetam.} }
\label{tw-96624aef945a}
\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.191\linewidth-2\tabcolsep}p{\dimexpr.6495\linewidth-2\tabcolsep}p{\dimexpr.15950000000000001\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}Analysts & \cAlignHack Mean area \ensuremath{\pm} Standard deviation* & \cAlignHack \%RSD\\
\tblmidrule 
Analyst 1 &
  \cAlignHack 103932\ensuremath{\pm}1001.01 &
  \cAlignHack 0.96\\
Analyst 2 &
  \cAlignHack 103895.3\ensuremath{\pm}987.67 &
  \cAlignHack 0.9\\
\tblbottomrule 
\end{tabulary}\par 
\end{table}
\textbf{Precision}

Precision of the system might have been performed Similarly as intraday precision, bury vivos trust day precision. With investigation the intraday precision, six replication standard results (20\ensuremath{\mu}g/ml) for Levetiracetam were injected. \% RSD might have been ascertained Furthermore it might have been found should a chance to be 1.029. Furthermore interday precision completed same as intraday, six replication standard results (20\ensuremath{\mu}g/ml) from claiming Levetiracetam were injected. \% RSD might have been computed and it might have been discovered on be 0. 969 which would great inside the satisfactory criteria from claiming not more than 2.0. Effects about framework precision investigations \unskip~\citep{963646:21047380}. 

\textbf{Accuracy}

Exactness of the strategy might have been examined by recuperation trials. Those recuperation investigations were performed by including referred to sums of the medications in the placebo. This recuperation might have been performed toward three levels, 50, 100 and 150\% of the name case of the tablet (500 mg from claiming Levetiracetam). The recuperation qualities for Levetiracetam went starting with 98.0 should 102.0\%. Those normal recoveries of three levels about Levetiracetam were found should be 99.7{\textendash}100.45\%. The outcomes are indicated in Table~\ref{tw-d50ec13e36ea}.

\textbf{Limit of identification and limit of quantification}

Those farthest point from claiming identification may be an explanatory technique will be those littlest measure about analyte done an example which could be dependable distinguished Toward the explanatory strategy \unskip~\citep{963646:21047383}. The breaking point from claiming quantitation is a distinctive explanatory methodology will be those littlest measure of the analyte Previously, test which might make quantitatively dead set. LOD and LOQ were ascertained utilizing equation

$\style{font-family:'Times New Roman'}{\mathrm{LOD}=3.3\left(\mathrm{SD}\right)/\mathrm S\;\mathrm{What}'\mathrm s\;\mathrm{more}\;\mathrm{LOQ}=10\left(\mathrm{SD}\right)/\mathrm S} $

\textbf{Toughness}

The toughness for test technique might have been showed by doing precision consider over six arrangements about example once an absolute clump test by separate analysts, those come about of the precision ponder are tabulated Similarly as underneath Table~\ref{tw-96624aef945a}. The \% RSD values are less than 2.
    
\section{Conclusions}
The current investigative technique is authenticated by ICH guidelines and met the acceptance criteria. It was concluded that the industrialized analytical technique is modest, precise, economical, and sensitive, and can be used for routine investigation of Levetiracetam in bulk drug and pharmaceutical dosage forms.

\textbf{Funding Support}

The authors declare that they have no funding support for this study.

\textbf{Conflict of Interest}

The authors declare that they have no conflict of interest for this study.
    

\bibliographystyle{pharmascope_apa-custom}

\bibliography{\jobname}
\end{document}
