Exploring its immunogenicity and antioxidant potential of protein from Mangifera indica and Syzygium cumini

Gurpreet Singh; Shubham Tyagi; Aashrika Gupta; Abhishekh Tripathi; Karishma Ghosh; Amit Gupta

Gurpreet Singh ⁽¹⁾ , Shubham Tyagi ⁽²⁾ , Aashrika Gupta ⁽³⁾ , Abhishekh Tripathi ⁽⁴⁾ , Karishma Ghosh ⁽⁵⁾ , Amit Gupta ⁽⁶⁾

(1) Department of Biotechnology, Graphic Era (Deemed to be) University, Dehradun, India, India ,

(2) Department of Biotechnology, Graphic Era (Deemed to be) University, Dehradun, India, India ,

(3) Department of Biotechnology, Graphic Era (Deemed to be) University, Dehradun, India, India ,

(4) Department of Biotechnology, Graphic Era (Deemed to be) University, Dehradun, India, India ,

(5) Department of Biotechnology, Graphic Era (Deemed to be) University, Dehradun, India, India ,

(6) Department of Biotechnology, Graphic Era (Deemed to be) University, Dehradun, India, India

Issue
Vol. 11 No. 3 (2020): Volume 11 Issue 3

Keywords:

Mangifera indica, Syzygium cumini, immunogenicity, antioxidant, protein

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Abstract

India is considered to be one of the rich repositories of medicinal and aromatic plants, which are mainly used as starting raw materials for drug manufacturing and perfumery products. More than thirty percent of various medicinal plant species are routinely used for various medicinal purposes. In this study, our major objective is to evaluate its immunogenicity and antioxidant potential of protein extracted from leaves of these two medicinal plants, i.e. Mangifera indica and Syzygium cumini. In order to achieve this objective, immunopharmacological studies were conducted and extracted protein from leaves (using Tris HCl and ice cold acetone) and confirming its protein content through the Lowry test. In addition, immunogenicity (using the standard vaccine, i.e. Typhoid vaccine) and antioxidant (using DPPH [1, 1-diphenyl-2- picrylhydrazyl] assay) based studies were evaluated using a variable concentration of protein. The results indicate that proteins showed immunogenic effect against typhoid vaccine and also showed antioxidant effect as compared to control. In short, proteins serve as a natural antioxidant and also showed immunogenicity, which may be useful in preventing free radical-induced diseases and also claimed its immunoboosting properties as well. Overall, these studies reveal that protein possesses potential benefits in terms of immunogenicity (against specific protein antigen) and antioxidant effect.