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\begin{document}


\def\RunningAuthor{Sujatha K et al.}
\firstPage{93}
\articleType{Original Article}
\receivedDate{19 Jul 2020}
\acceptedDate{06 Aug 2020}
\revisedDate{05 Aug 2020}
\journalVolume{2021, 12}
\journalIssue{1}
\journalDoi{ijrps.v12i1.3940}
\copyrightYear{2021}
\def\authorCount{3}
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\def\journalTitle{International Journal of Research in Pharmaceutical Sciences}

\title{\textbf{A Novel and Rapid RP-HPLC Quantitative Method for the estimation of Canagliflozin in Human Plasma}}
\author{Ajitha~A\textsuperscript{1},  
            Sujatha~K\textsuperscript{*}\textsuperscript{2}, 
            Abbulu~K\textsuperscript{3}~\\[5pt]{\textsuperscript{1}Department of Pharmaceutical Analysis, CMR college of Pharmacy, Kandlakoya (V), Medchal Road, Hyderabad - 501 401, India (ORCID ID-0000-0003-0270-2842)}~\\{\textsuperscript{2}Department of Pharmaceutical Chemistry, Sri Ramachandra Faculty of pharmacy, Sri Ramachandra Institute of Higher Education and Research (Deemed to be University), No.1 Ramachandra Nagar, Porur, Chennai - 600 116 India. (ORCID ID-0000-0001-6130-5412)}~\\{\textsuperscript{3}Department of Pharmaceutics, CMR college of Pharmacy, Kandlakoya (V), Medchal Road, Hyderabad - 501 401,India. (ORCID ID-0000-0002-3932-4315)}}

\begin{abstract}
A simple, precise and accurate method was developed for the quantitative estimation of Canagliflozin in human plasma using Dapagliflozin as internal standard by Reverse Phase-High Performance Liquid Chromatographic technique. Chromatographic conditions were of stationary phase Phenomenex Luna C18 (2) (150 x 4.6 mm, 5m), Mobile phase 0.01 N Potassium dihydrogen Phosphate buffer pH (3.5\ensuremath{\pm}0.05) : acetonitrile in the ratio of 45:55 and flow rate at 1.0 ml/min, detection wave length was UV 222 nm, column oven temperature was maintained at 30\textsuperscript{o}C, and sample injection volume of 10 \ensuremath{\mu}L. Retention time of Canagliflozin was found to be about 8.7 min. Coefficient of Variation for Canagliflozin peak was 3.15 \%, \% recovery was 94.58 \%. The linearity of method was studied from 0.06 \ensuremath{\mu}g/ml to 2.4 \ensuremath{\mu}g/ml (R\ensuremath{^{2}} = 0.999). The Signal to Noise ratio of lower limit of quantification (0.06 \ensuremath{\mu}g/ml) was found to be 50. The proposed bio-analytical method was validated by following ICH guidelines.
\end{abstract}\def\keywordstitle{Keywords}

\begin{keywords}Canagliflozin,\newline Human Plasma,\newline Validation,\newline ICH Guidelines
\end{keywords}
\twocolumn[ \maketitle {\printKwdAbsBox}]
\makeatletter{\raggedright\textsuperscript{*}Corresponding Author\par Name:\ Sujatha~K~\\ Phone:\ +91 9444481844~\\  Email:\ sujamano73@gmail.com
        \par}\vspace*{-11pt}\hrulefill\par{\fontsize{12}{14}\selectfont ISSN: 0975-7538}\par% 
        \textsc{DOI:}\ \href{https://doi.org/10.26452/\@journalDoi}{\textcolor{blue}{\underline{\smash{https://doi.org/10.26452/\@journalDoi}}}}\par%
        \vspace*{-11pt}\hrulefill\\{\fontsize{9.12}{10.12}\selectfont Production and Hosted by}\par{IJRPS |~\href{www.ijrps.com}{www.ijrps.com}}\par%
        \vspace*{-2pt}{\fontsize{9.12}{10.12}\selectfont\textcopyright\ \@copyrightYear\ $|$ All rights reserved.}\par%
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        \makeatother
        
    
\section{Introduction}
Diabetes mellitus affects 425 million people in the world, which accounts for this number rising to around 629 million diabetes globally by 2045 \unskip~\citep{861407:20147960}. Sodium-Glucose co-transporter 2 (SGLT2) inhibitors are a novel approach in the treatment plan of type 2 diabetes mellitus \unskip~\citep{861407:20147972}. Canagliflozin is the first SGLT2 inhibitor available on the market (2015) which reduces systolic blood pressure and has a low risk of hypoglycemia \unskip~\citep{861407:20147965}. Canagliflozin hemihydrate (Figure~\ref{f-87e2dcd38645}) is chemically 1-(beta-d-glucopyranosyl)-4-methyl-3-[5-(4-fluorophenyl)-2-thienylmethyl]benzene hemihydrates with a molecular weight of 453.52 g/mol. For the quantification of Canagliflozin in pharmaceutical dosage forms and biological fluids only few analytical methods like LC-MS/MS \unskip~\citep{861407:20147971}, UV \unskip~\citep{861407:20147970}, RP-HPLC \unskip~\citep{861407:20147967,861407:20147961,861407:20147966,861407:20147968,861407:20147963,861407:20147969}, HPTLC method \unskip~\citep{861407:20147962,861407:20147964} are reported. Simple, rapid (to reduce analytical down time in turn revenue) and cost effective bio-analytical method development is a thirst area of importance in the Pharmaceutical Industry. Hence a simple, precise, accurate, sensitive, selective and robust RP-HPLC bioanalytical method was developed and validated as per ICH guideline for the estimation of Canagliflozin, in human plasma is reported in this article.
\bgroup
\fixFloatSize{images/04396b28-faa2-4d12-bf7a-6c5615fe119b-upic-1.png}
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\centering \makeatletter\IfFileExists{images/04396b28-faa2-4d12-bf7a-6c5615fe119b-upic-1.png}{\includegraphics{images/04396b28-faa2-4d12-bf7a-6c5615fe119b-upic-1.png}}{}
\makeatother 
\caption{\boldmath {Structural Formula of Canagliflozin hemihydrate}}
\label{f-87e2dcd38645}
\end{figure}
\egroup

    
\section{Materials and Methods}
\textbf{Reagents and Chemicals } Canagliflozin hemihydrate and Dapagliflozin were obtained as gift samples. HPLC grade Acetonitrile and water were purchased from the local vendor (Make: Merck, Mumbai, India). Potassium di-hydrogen phosphate (ACS grade) and ortho-phosphoric acid (ACS Grade) were procured from the local supplier (Make: Rankem, Hyderabad, India).


\bgroup
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\centering \makeatletter\IfFileExists{images/d7680fbe-f9af-447d-85db-9afa7af9e3be-upic-2.png}{\includegraphics{images/d7680fbe-f9af-447d-85db-9afa7af9e3be-upic-2.png}}{}
\makeatother 
\caption{\boldmath {Typical Chromatogram of MQC Sample}}
\label{f-1012b1a51437}
\end{figure}
\egroup

\bgroup
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\begin{figure}[!htbp]
\centering \makeatletter\IfFileExists{images/4b739a0b-ff56-4238-afa7-7dbe08377d95-upic-3.png}{\includegraphics{images/4b739a0b-ff56-4238-afa7-7dbe08377d95-upic-3.png}}{}
\makeatother 
\caption{\boldmath {Representative Chromatogram of plasma extracted blank}}
\label{f-f5d1053c2469}
\end{figure}
\egroup
\textbf{Instrumentation and Chromatographic Condition}

Chromatographic analysis was performed on Waters Alliance HPLC 2695 model equipped with quaternary pumps, Photodiode array detector and auto sampler. Empower 2 Software is used thorough out the analysis. The HPLC column used was  Phenomenex Luna C18 (2) (4.6 x 150 mm, 5m). The mobile phase consist of 0.01N potassium di-hydrogen phosphate in water (pH 3.5\ensuremath{\pm}0.04 adjusted with 10\% ortho-phosphoric acid solution) and Acetonitrile in the ratio of (45:55) \% v/v. Filtered the mobile phase through 0.45 \ensuremath{\mu} membrane filter under vacuum and degassed. Mobile phase flow rate was set at 1.0 ml/min and column oven temperature was set at 30\ensuremath{^\circ}\ensuremath{\pm}2\ensuremath{^\circ}C. The detector wavelength was set at UV 222 nm. The auto-sample cooler temperature was set at 5\ensuremath{^\circ}\ensuremath{\pm}2\ensuremath{^\circ}C. Chromatographic run time was monitored for 15 min.


\bgroup
\fixFloatSize{images/fcf40e40-92bf-474e-9a8e-1fb910094663-upic-4.png}
\begin{figure}[!htbp]
\centering \makeatletter\IfFileExists{images/fcf40e40-92bf-474e-9a8e-1fb910094663-upic-4.png}{\includegraphics{images/fcf40e40-92bf-474e-9a8e-1fb910094663-upic-4.png}}{}
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\caption{\boldmath {Representative Chromatogram of Internal standard solution (Dapagliflozin)}}
\label{f-297be67985e2}
\end{figure}
\egroup

\bgroup
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\begin{figure}[!htbp]
\centering \makeatletter\IfFileExists{images/526e93b2-d609-4608-9dd7-c646ebf790bd-upic-5.png}{\includegraphics{images/526e93b2-d609-4608-9dd7-c646ebf790bd-upic-5.png}}{}
\makeatother 
\caption{\boldmath {Calibration Curve for Canagliflozin}}
\label{f-c4b54c556646}
\end{figure}
\egroup
\textbf{Preparation of Solutions}

\textbf{Preparation of diluent }Mixture of 0.01N potassium di-hydrogen phosphate in water (pH 3.5\ensuremath{\pm}0.1 


\begin{table*}[!htbp]
\caption{\boldmath {Evaluation of System Suitability} }
\label{tw-6aa3b7766b62}
\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.0839\linewidth-2\tabcolsep}p{\dimexpr.17110000000000003\linewidth-2\tabcolsep}p{\dimexpr.1918\linewidth-2\tabcolsep}p{\dimexpr.1919\linewidth-2\tabcolsep}p{\dimexpr.1877\linewidth-2\tabcolsep}p{\dimexpr.1736\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}\cAlignHack S No & \cAlignHack Analyte (Canagliflozin) Area & \cAlignHack Retention Time of Canagliflozin (min) & \cAlignHack Internal Standard (Dapagliflozin) Area & \cAlignHack Retention Time for Internal Standard (Dapagliflozin) (min) & \cAlignHack Area Ratio (Analyte / Internal Standard)\\
\tblmidrule 
\cAlignHack 1 &
  \cAlignHack 135280 &
  \cAlignHack 8.75 &
  \cAlignHack 1073287 &
  \cAlignHack 4.15 &
  \cAlignHack 0.1260\\
\cAlignHack 2 &
  \cAlignHack 136747 &
  \cAlignHack 8.75 &
  \cAlignHack 1066933 &
  \cAlignHack 4.15 &
  \cAlignHack 0.1282\\
\cAlignHack 3 &
  \cAlignHack 131298 &
  \cAlignHack 8.77 &
  \cAlignHack 1063306 &
  \cAlignHack 4.15 &
  \cAlignHack 0.1235\\
\cAlignHack 4 &
  \cAlignHack 134214 &
  \cAlignHack 8.77 &
  \cAlignHack 1046126 &
  \cAlignHack 4.15 &
  \cAlignHack 0.1283\\
\cAlignHack 5 &
  \cAlignHack 137520 &
  \cAlignHack 8.78 &
  \cAlignHack 1061621 &
  \cAlignHack 4.16 &
  \cAlignHack 0.1295\\
\cAlignHack 6 &
  \cAlignHack 134681 &
  \cAlignHack 8.78 &
  \cAlignHack 1050177 &
  \cAlignHack 4.16 &
  \cAlignHack 0.1282\\
\multicolumn{2}{p{\dimexpr(.25500000000000004\linewidth-2\tabcolsep)}}{\cAlignHack \textbf{Mean Area}} &
  \cAlignHack 8.767 &
  \multicolumn{1}{p{\dimexpr(.1919\linewidth-2\tabcolsep)}}{\multirow{3}{\linewidth}{\cAlignHack NA}} &
  \cAlignHack 4.153 &
  \cAlignHack 0.1273\\
\multicolumn{2}{p{\dimexpr(.25500000000000004\linewidth-2\tabcolsep)}}{\cAlignHack \textbf{SD }} &
  \cAlignHack 0.0173 &
   &
  \cAlignHack 0.0055 &
  \cAlignHack 0.00218\\
\multicolumn{2}{p{\dimexpr(.25500000000000004\linewidth-2\tabcolsep)}}{\cAlignHack \textbf{\%CV}} &
  \cAlignHack 0.20 &
   &
  \cAlignHack 0.13 &
  \cAlignHack 1.71\\
\tblbottomrule 
\end{tabulary}\par 
\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {Data for Calibration Curve of Canagliflozin} }
\label{tw-fc641ccd573e}
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\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.1396\linewidth-2\tabcolsep}p{\dimexpr.11459999999999999\linewidth-2\tabcolsep}p{\dimexpr.10719999999999999\linewidth-2\tabcolsep}p{\dimexpr.11459999999999999\linewidth-2\tabcolsep}p{\dimexpr.10440000000000003\linewidth-2\tabcolsep}p{\dimexpr.11009999999999998\linewidth-2\tabcolsep}p{\dimexpr.1035\linewidth-2\tabcolsep}p{\dimexpr.10440000000000001\linewidth-2\tabcolsep}p{\dimexpr.10159999999999998\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}{\multirow{2}{*}{S No.}} & Standard Solution-1  & Standard Solution-2 & Standard Solution-3 & Standard Solution-4  & Standard Solution-5 & Standard Solution-6 & Standard Solution-7 & Standard Solution-8\\
\rowcolor{kwdboxcolor} & \multicolumn{8}{p{\dimexpr(.8603999999999999\linewidth-2\tabcolsep)}}{\cAlignHack Concentration Found (\ensuremath{\mu}g/ml)}\\
\tblmidrule 
1 &
  \cAlignHack 0.057 &
  \cAlignHack 0.118 &
  \cAlignHack 0.177 &
  \cAlignHack 0.476 &
  \cAlignHack 1.181 &
  \cAlignHack 1.428 &
  \cAlignHack 1.918 &
  \cAlignHack 2.386\\
2 &
  \cAlignHack 0.063 &
  \cAlignHack 0.111 &
  \cAlignHack 0.182 &
  \cAlignHack 0.480 &
  \cAlignHack 1.194 &
  \cAlignHack 1.432 &
  \cAlignHack 1.911 &
  \cAlignHack 2.399\\
3 &
  \cAlignHack 0.059 &
  \cAlignHack 0.121 &
  \cAlignHack 0.169 &
  \cAlignHack 0.486 &
  \cAlignHack 1.203 &
  \cAlignHack 1.446 &
  \cAlignHack 1.921 &
  \cAlignHack 2.462\\
\textbf{Mean \mbox{}\protect\newline Concentration} &
  \cAlignHack 0.0596 &
  \cAlignHack 0.1166 &
  \cAlignHack 0.1760 &
  \cAlignHack 0.4806 &
  \cAlignHack 1.192 &
  \cAlignHack 1.4353 &
  \cAlignHack 1.9166 &
  \cAlignHack 2.415\\
\textbf{SD} &
  \cAlignHack 0.0031 &
  \cAlignHack 0.0052 &
  \cAlignHack 0.0066 &
  \cAlignHack 0.0051 &
  \cAlignHack 0.0111 &
  \cAlignHack 0.0095 &
  \cAlignHack 0.0052 &
  \cAlignHack 0.0406\\
\textbf{\%CV} &
  \cAlignHack 5.12 &
  \cAlignHack 4.40 &
  \cAlignHack 3.73 &
  \cAlignHack 1.05 &
  \cAlignHack 0.93 &
  \cAlignHack 0.66 &
  \cAlignHack 0.27 &
  \cAlignHack 1.68\\
\textbf{Mean Accuracy (\%)} &
  \cAlignHack 99.44 &
  \cAlignHack 97.22 &
  \cAlignHack 97.78 &
  \cAlignHack 100.14 &
  \cAlignHack 99.39 &
  \cAlignHack 99.68 &
  \cAlignHack 99.83 &
  \cAlignHack 100.65\\
\tblbottomrule 
\end{tabulary}\par 
\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {With-in run Accuracy and Precision data for Canagliflozin} }
\label{tw-fdcc53ac050a}
\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.205\linewidth-2\tabcolsep}p{\dimexpr.18840000000000003\linewidth-2\tabcolsep}p{\dimexpr.19670000000000016\linewidth-2\tabcolsep}p{\dimexpr.2065999999999999\linewidth-2\tabcolsep}p{\dimexpr.2032999999999999\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}{\multirow{2}{*}{S No.}} & HQC(1.9 \ensuremath{\mu}g/ml\textbf{)} & MQC(1.2 \ensuremath{\mu}g/ml) & LQC(0.18 \ensuremath{\mu}g/ml) & LLOQC \mbox{}\protect\newline (0.06 \ensuremath{\mu}g/ml\textbf{)}\\
\rowcolor{kwdboxcolor} & \multicolumn{4}{p{\dimexpr(.795\linewidth-2\tabcolsep)}}{\cAlignHack Concentration Found (\ensuremath{\mu}g/ml)}\\
\tblmidrule 
1 &
  1.915 &
  1.181 &
  0.177 &
  0.058\\
2 &
  1.917 &
  1.194 &
  0.182 &
  0.062\\
3 &
  1.913 &
  1.203 &
  0.173 &
  0.052\\
4 &
  1.911 &
  1.185 &
  0.170 &
  0.056\\
5 &
  1.922 &
  1.198 &
  0.175 &
  0.059\\
6 &
  1.912 &
  1.189 &
  0.171 &
  0.063\\
\textbf{Mean} \mbox{}\protect\newline \textbf{Concentration} \mbox{}\protect\newline \textbf{(\ensuremath{\mu}g/ml)} &
  1.9150 &
  1.1916 &
  0.1746 &
  0.0583\\
\textbf{SD} &
  0.0041 &
  0.0083 &
  0.0045 &
  0.0041\\
\textbf{\%CV} &
  0.21 &
  0.69 &
  2.53 &
  6.91\\
\textbf{Mean Accuracy (\%)} &
  99.74 &
  99.31 &
  97.04 &
  97.22\\
\tblbottomrule 
\end{tabulary}\par 
\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {With-in run Accuracy and Precision data for Canagliflozin} }
\label{tw-d780c865b618}
\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.2117\linewidth-2\tabcolsep}p{\dimexpr.18169999999999998\linewidth-2\tabcolsep}p{\dimexpr.19840000000000003\linewidth-2\tabcolsep}p{\dimexpr.22159999999999997\linewidth-2\tabcolsep}p{\dimexpr.1866\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}{\multirow{2}{*}{S No}} & HQC(1.9 \ensuremath{\mu}g/ml) & MQC(1.2 \ensuremath{\mu}g/ml) & LQC(0.18 \ensuremath{\mu}g/ml) & LLOQC \mbox{}\protect\newline (0.06 \ensuremath{\mu}g/ml)\\
\rowcolor{kwdboxcolor} & \multicolumn{4}{p{\dimexpr(.7883\linewidth-2\tabcolsep)}}{\cAlignHack Concentration Found (\ensuremath{\mu}g/ml)}\\
\tblmidrule 
1 &
  1.910 &
  1.198 &
  0.172 &
  0.057\\
2 &
  1.915 &
  1.195 &
  0.180 &
  0.058\\
3 &
  1.920 &
  1.189 &
  0.173 &
  0.063\\
4 &
  1.908 &
  1.193 &
  0.175 &
  0.062\\
5 &
  1.922 &
  1.196 &
  0.174 &
  0.055\\
6 &
  1.918 &
  1.194 &
  0.182 &
  0.059\\
\textbf{Mean \mbox{}\protect\newline concentration \mbox{}\protect\newline (\ensuremath{\mu}g/ml)} &
  1.9155 &
  1.1941 &
  0.1760 &
  0.0590\\
\textbf{SD} &
  0.0056 &
  0.0031 &
  0.0041 &
  0.0031\\
\textbf{\%CV} &
  0.29 &
  0.26 &
  2.30 &
  5.14\\
\textbf{Mean Accuracy} &
  99.77 &
  99.51 &
  97.78 &
  98.33\\
\tblbottomrule 
\end{tabulary}\par 
\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {With-in run Accuracy and Precisiondata for Canagliflozin} }
\label{tw-f45183fb4d03}
\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.195\linewidth-2\tabcolsep}p{\dimexpr.195\linewidth-2\tabcolsep}p{\dimexpr.20169999999999995\linewidth-2\tabcolsep}p{\dimexpr.22160000000000004\linewidth-2\tabcolsep}p{\dimexpr.1867\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}S. No & HQC(1.9 \ensuremath{\mu}g/ml) & MQC(1.2 \ensuremath{\mu}g/ml) & LQC(0.18 \ensuremath{\mu}g/ml) & LLOQC \mbox{}\protect\newline (0.06 \ensuremath{\mu}g/ml)\\
\rowcolor{kwdboxcolor} & \multicolumn{3}{p{\dimexpr(.6183\linewidth-2\tabcolsep)}}{\cAlignHack Concentration Found (\ensuremath{\mu}g/ml)} & \\
\tblmidrule 
1 &
  1.917 &
  1.191 &
  0.178 &
  0.062\\
2 &
  1.921 &
  1.203 &
  0.182 &
  0.058\\
3 &
  1.915 &
  1.195 &
  0.184 &
  0.055\\
4 &
  1.918 &
  1.197 &
  0.176 &
  0.063\\
5 &
  1.923 &
  1.193 &
  0.174 &
  0.056\\
6 &
  1.916 &
  1.189 &
  0.172 &
  0.059\\
\textbf{Mean \mbox{}\protect\newline Concentration \mbox{}\protect\newline (\ensuremath{\mu}g/ml)} &
  1.9183 &
  1.1946 &
  0.1776 &
  0.0588\\
\textbf{SD} &
  0.0031 &
  0.0050 &
  0.0047 &
  0.0032\\
\textbf{\%CV} &
  0.16 &
  0.42 &
  2.61 &
  5.42\\
\textbf{\% Mean \mbox{}\protect\newline Accuracy} &
  99.91 &
  99.91 &
  98.70 &
  98.06\\
\multicolumn{5}{p{\dimexpr(1\linewidth-2\tabcolsep)}}{\cAlignHack \textbf{Between Batch Accuracy and precision}}\\
\textbf{ N} &
  18 &
  18 &
  18 &
  18\\
\textbf{Mean \mbox{}\protect\newline Concentration \mbox{}\protect\newline (\ensuremath{\mu}g/ml)} &
  1.9162 &
  1.1935 &
  1.7611 &
  0.0587\\
\textbf{SD} &
  0.0044 &
  0.0057 &
  0.0043 &
  0.0033\\
\textbf{\%CV} &
  0.23 &
  0.47 &
  2.44 &
  5.54\\
\textbf{\% Mean \mbox{}\protect\newline Accuracy} &
  99.81 &
  99.46 &
  97.84 &
  97.87\\
\tblbottomrule 
\end{tabulary}\par 
\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {Between run Accuracy and Precision data for Canagliflozin} }
\label{tw-43ddb48c533e}
\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.060900000000000025\linewidth-2\tabcolsep}p{\dimexpr.2801\linewidth-2\tabcolsep}p{\dimexpr.15579999999999993\linewidth-2\tabcolsep}p{\dimexpr.16439999999999998\linewidth-2\tabcolsep}p{\dimexpr.1681\linewidth-2\tabcolsep}p{\dimexpr.17070000000000007\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}{\multirow{2}{*}{S No}} & {\multirow{2}{*}{\cAlignHack Change of HPLC \mbox{}\protect\newline Column ID}} & HQC \mbox{}\protect\newline (1.9 \ensuremath{\mu}g/ml) \mbox{}\protect\newline  & MQC \mbox{}\protect\newline (1.2 \ensuremath{\mu}g/ml) & LQC \mbox{}\protect\newline (0.18 \ensuremath{\mu}g/ml) & LLOQC \mbox{}\protect\newline (0.06 \ensuremath{\mu}g/ml)\\
\rowcolor{kwdboxcolor} &  & \multicolumn{4}{p{\dimexpr(.6589999999999999\linewidth-2\tabcolsep)}}{\cAlignHack Concentration Found (\ensuremath{\mu}g/ml)}\\
\tblmidrule 
1 &
  \multicolumn{1}{p{\dimexpr(.2801\linewidth-2\tabcolsep)}}{\multirow{6}{\linewidth}{\cAlignHack \textbf{HPLC Column 2 }}} &
  1.917 &
  1.196 &
  0.176 &
  0.056\\
2 &
   &
  1.923 &
  1.198 &
  0.183 &
  0.058\\
3 &
   &
  1.916 &
  1.203 &
  0.177 &
  0.062\\
4 &
   &
  1.919 &
  1.201 &
  0.179 &
  0.056\\
5 &
   &
  1.920 &
  1.197 &
  0.181 &
  0.060\\
6 &
   &
  1.924 &
  1.194 &
  0.178 &
  0.058\\
\multicolumn{2}{p{\dimexpr(.341\linewidth-2\tabcolsep)}}{\textbf{ N}} &
  6 &
  6 &
  6 &
  6\\
\multicolumn{2}{p{\dimexpr(.341\linewidth-2\tabcolsep)}}{\textbf{Mean Concentration \mbox{}\protect\newline (\ensuremath{\mu}g/ml)}} &
  1.9198 &
  1.1981 &
  0.179 &
  0.0583\\
\multicolumn{2}{p{\dimexpr(.341\linewidth-2\tabcolsep)}}{\textbf{SD}} &
  0.0032 &
  0.0034 &
  0.0027 &
  0.0024\\
\multicolumn{2}{p{\dimexpr(.341\linewidth-2\tabcolsep)}}{\textbf{\% CV}} &
  0.17 &
  0.28 &
  1.46 &
  4.01\\
\multicolumn{2}{p{\dimexpr(.341\linewidth-2\tabcolsep)}}{\textbf{\% Mean Accuracy}} &
  99.99 &
  99.85 &
  99.44 &
  97.22\\
\tblbottomrule 
\end{tabulary}\par 
\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {Between run Precision data for Canagliflozin.} }
\label{tw-038a76a20fb5}
\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.06999999999999999\linewidth-2\tabcolsep}p{\dimexpr.22100000000000005\linewidth-2\tabcolsep}p{\dimexpr.1739\linewidth-2\tabcolsep}p{\dimexpr.17509999999999998\linewidth-2\tabcolsep}p{\dimexpr.1724\linewidth-2\tabcolsep}p{\dimexpr.1876\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}{\multirow{2}{*}{\textbf{S N}}} & {\multirow{2}{*}{Change of Analyst}} & HQC \mbox{}\protect\newline (1.9 \ensuremath{\mu}g/ml) & MQC \mbox{}\protect\newline (1.2 \ensuremath{\mu}g/ml) & LQC \mbox{}\protect\newline (0.18 \ensuremath{\mu}g/ml) & LLOQC \mbox{}\protect\newline (0.06 \ensuremath{\mu}g/ml)\\
\rowcolor{kwdboxcolor} &  & \multicolumn{4}{p{\dimexpr(.709\linewidth-2\tabcolsep)}}{\cAlignHack Concentration Found (\ensuremath{\mu}g/ml)}\\
\tblmidrule 
1 &
  \multicolumn{1}{p{\dimexpr(.22100000000000005\linewidth-2\tabcolsep)}}{\multirow{6}{\linewidth}{\cAlignHack \textbf{Analyst-2}}} &
  1.921 &
  1.202 &
  0.182 &
  0.062\\
2 &
   &
  1.917 &
  1.197 &
  0.177 &
  0.058\\
3 &
   &
  1.919 &
  1.195 &
  0.174 &
  0.061\\
4 &
   &
  1.923 &
  1.204 &
  0.183 &
  0.057\\
5 &
   &
  1.916 &
  1.198 &
  0.178 &
  0.063\\
6 &
   &
  1.918 &
  1.196 &
  0.182 &
  0.059\\
\multicolumn{2}{p{\dimexpr(.29100000000000005\linewidth-2\tabcolsep)}}{\textbf{ N}} &
  6 &
  6 &
  6 &
  6\\
\multicolumn{2}{p{\dimexpr(.29100000000000005\linewidth-2\tabcolsep)}}{\textbf{Mean Concentration (\ensuremath{\mu}g/ml)}} &
  1.919 &
  1.1986 &
  0.1793 &
  0.060\\
\multicolumn{2}{p{\dimexpr(.29100000000000005\linewidth-2\tabcolsep)}}{\textbf{SD}} &
  0.0027 &
  0.0036 &
  0.0036 &
  0.0024\\
\multicolumn{2}{p{\dimexpr(.29100000000000005\linewidth-2\tabcolsep)}}{\textbf{\% CV}} &
  0.14 &
  0.30 &
  1.98 &
  3.94\\
\multicolumn{2}{p{\dimexpr(.29100000000000005\linewidth-2\tabcolsep)}}{\textbf{\% Mean Accuracy}} &
  99.95 &
  99.89 &
  99.63 &
  100.00\\
\tblbottomrule 
\end{tabulary}\par 
\end{table*}
adjusted with 10\% ortho-phosphoric acid solution): Acetonitrile in the ratio of 50:50 \% v/v.

\textbf{Preparation of Canagliflozin Stock solution (120 \ensuremath{\mu}g/ml) }Accurately weighed and transferred 2 mg of Canagliflozin hemihydrate into a 200 mL volumetric flask, added 120 mL of diluent and sonicated for 5 minutes. Diluted up to the mark with diluent and mixed (120 \ensuremath{\mu}g/ml of Canagliflozin).

\textbf{Preparation of Canagliflozin Spiking Solutions (0.06 \ensuremath{\mu}g/ml to 2.4 \ensuremath{\mu}g/ml) }From the above Canagliflozin stock solution 0.1ml, 0.2ml, 0.3ml, 1.2ml, 2.0ml, 2.4ml, 3.2ml and 4.0 ml was transferred using micro pipette into 8 individual 20 ml volumetric flask and make up the volume up to the mark with diluent to produce 0.6 \ensuremath{\mu}g/ml, 1.2 \ensuremath{\mu}g/ml, 1.8 \ensuremath{\mu}g/ml, 4.8 \ensuremath{\mu}g/ml, 12.0 \ensuremath{\mu}g/ml, 14.4 \ensuremath{\mu}g/ml, 19.2 \ensuremath{\mu}g/ml and 24 \ensuremath{\mu}g/ml.

\textbf{Preparation of Calibration Standards and Quality control samples }Calibration standards and quality control (QC) samples were made by mixing blank human plasma with Canagliflozin Spiking Solution to get 0.06 \ensuremath{\mu}g/ml, 0.12  \ensuremath{\mu}g/ml, 0.18  \ensuremath{\mu}g/ml, 0.48  \ensuremath{\mu}g/ml, 1.2  \ensuremath{\mu}g/ml, 1.44  \ensuremath{\mu}g/ml, 1.92  \ensuremath{\mu}g/ml and 2.4  \ensuremath{\mu}g/ml. (of Canagliflozin)

\textbf{Preparation of internal standard Solution (5} \textbf{\ensuremath{\mu}g/ml) }Accurately weighed and transferred 12.5 mg of Dapagliflozin in to a 50 ml volumetric flask added 30 ml of diluent and sonicated for 5 minutes and 


\begin{table*}[!htbp]
\caption{\boldmath {Recovery- Study of Canagliflozin from Human Plasma.} }
\label{tw-bd3f577b585a}
\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.1815\linewidth-2\tabcolsep}p{\dimexpr.13170000000000002\linewidth-2\tabcolsep}p{\dimexpr.13400000000000002\linewidth-2\tabcolsep}p{\dimexpr.1354\linewidth-2\tabcolsep}p{\dimexpr.13999999999999998\linewidth-2\tabcolsep}p{\dimexpr.12690000000000001\linewidth-2\tabcolsep}p{\dimexpr.1505\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}{\multirow{3}{*}{S No}} & \multicolumn{2}{p{\dimexpr(.26570000000000004\linewidth-2\tabcolsep)}}{\cAlignHack HQC (1.9 \ensuremath{\mu}g/ml)} & \multicolumn{2}{p{\dimexpr(.2754\linewidth-2\tabcolsep)}}{\cAlignHack MQC (1.2 \ensuremath{\mu}g/ml)} & \multicolumn{2}{p{\dimexpr(.27740000000000002\linewidth-2\tabcolsep)}}{\cAlignHack LQC (0.18 \ensuremath{\mu}g/ml)}\\
\rowcolor{kwdboxcolor} & \multicolumn{6}{p{\dimexpr(.8185\linewidth-2\tabcolsep)}}{\cAlignHack Response (area) for}\\
\rowcolor{kwdboxcolor} & Un-extracted sample & \cAlignHack Extracted \mbox{}\protect\newline sample & Un-extracted sample & \cAlignHack Extracted sample & Un-extracted sample & \cAlignHack Extracted sample\\
\tblmidrule 
1 &
  230567 &
  213384 &
  138082 &
  135494 &
  22140 &
  20375\\
2 &
  231516 &
  213162 &
  138498 &
  136540 &
  21969 &
  20501\\
3 &
  232044 &
  212871 &
  137951 &
  135497 &
  21940 &
  19889\\
4 &
  230181 &
  212687 &
  138808 &
  134652 &
  21849 &
  20443\\
5 &
  232393 &
  213934 &
  137521 &
  135384 &
  21835 &
  21381\\
6 &
  233095 &
  217481 &
  138134 &
  134545 &
  22022 &
  20501\\
\textbf{N } &
  \textbf{6} &
  \textbf{6} &
  \textbf{6} &
  \textbf{6} &
  \textbf{6} &
  \textbf{6}\\
\textbf{Mean Area} &
  231633 &
  213920 &
  138166 &
  135352 &
  21959 &
  20515\\
\textbf{SD} &
  1108.36 &
  1797.97 &
  445.58 &
  720.39 &
  113.72 &
  483.12\\
\textbf{\% CV} &
  \textbf{0.48} &
  \textbf{0.84} &
  \textbf{0.32} &
  \textbf{0.53} &
  \textbf{0.52} &
  \textbf{2.35}\\
\textbf{Mean Recovery (\%)} &
  \multicolumn{2}{p{\dimexpr(.26570000000000004\linewidth-2\tabcolsep)}}{92.35} &
  \multicolumn{2}{p{\dimexpr(.2754\linewidth-2\tabcolsep)}}{97.96} &
  \multicolumn{2}{p{\dimexpr(.27740000000000002\linewidth-2\tabcolsep)}}{93.42}\\
\textbf{Overall Mean Recovery (\%)} &
  \multicolumn{6}{p{\dimexpr(.8185\linewidth-2\tabcolsep)}}{94.580}\\
\textbf{Overall SD} &
  \multicolumn{6}{p{\dimexpr(.8185\linewidth-2\tabcolsep)}}{2.9787}\\
\textbf{Overall CV (\%)} &
  \multicolumn{6}{p{\dimexpr(.8185\linewidth-2\tabcolsep)}}{3.15}\\
\tblbottomrule 
\end{tabulary}\par 
\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {The Matrix sample long term stability of Canagliflozin at -28 \ensuremath{\pm} 5\ensuremath{^\circ}C (60 Days).} }
\label{tw-843449f23aa6}
\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{LLLLL}
\tbltoprule \rowcolor{kwdboxcolor}{\multirow{3}{*}{S No}} & \multicolumn{2}{p{\dimexpr(\mcWidth{2}+\mcWidth{3})}}{\cAlignHack HQC (1.9 \ensuremath{\mu}g/mL)} & \multicolumn{2}{p{\dimexpr(\mcWidth{4}+\mcWidth{5})}}{\cAlignHack LQC (0.18 \ensuremath{\mu}g/mL)}\\
\rowcolor{kwdboxcolor} & \multicolumn{4}{p{\dimexpr(\mcWidth{2}+\mcWidth{3}+\mcWidth{4}+\mcWidth{5})}}{\cAlignHack Concentration Found (\ensuremath{\mu}g/ml)}\\
\rowcolor{kwdboxcolor} & Control Sample  & Stability sample & Control Sample & Stability sample\\
\tblmidrule 
1 &
  1.917 &
  1.923 &
  0.177 &
  0.175\\
2 &
  1.920 &
  1.915 &
  0.182 &
  0.185\\
3 &
  1.923 &
  1.921 &
  0.176 &
  0.182\\
4 &
  1.916 &
  1.915 &
  0.178 &
  0.178\\
5 &
  1.918 &
  1.922 &
  0.183 &
  0.176\\
6 &
  1.922 &
  1.919 &
  0.181 &
  0.179\\
\textbf{Mean Concentration } &
  1.9193 &
  1.9191 &
  0.1795 &
  0.1791\\
\textbf{SD} &
  0.0029 &
  0.0035 &
  0.0029 &
  0.0038\\
\textbf{\% CV} &
  \textbf{0.15} &
  \textbf{0.18} &
  \textbf{1.60} &
  \textbf{2.10}\\
\textbf{Mean accuracy (\%)} &
  \textbf{99.97} &
  \textbf{99.96} &
  \textbf{99.72} &
  \textbf{99.54}\\
\textbf{Mean stability (\%)} &
  \multicolumn{2}{p{\dimexpr(\mcWidth{2}+\mcWidth{3})}}{\textbf{99.96}} &
  \multicolumn{2}{p{\dimexpr(\mcWidth{4}+\mcWidth{5})}}{\textbf{99.63}}\\
\tblbottomrule 
\end{tabulary}\par 
\end{table*}
diluted up to the mark with diluent and mixed (250 \ensuremath{\mu}g/ml of Dapagliflozin). Further diluted 1.0 ml of above solution to 10 ml with diluent and mixed (25 \ensuremath{\mu}g/ml of Dapagliflozin).

NOTE: 0.5ml (500 \ensuremath{\mu}l) of the above internal standard solution is to be mixed with spiking blank plasma and working stock dilutions of analyte to get 5 \ensuremath{\mu}g/ml Dapagliflozin (internal standard).\textbf{\space }

\textbf{Extraction procedure  }Using a 1.0 ml micro pipette transferred 750 \ensuremath{\mu}L of human plasma and 500 \ensuremath{\mu}L of internal standard solution, 250 \ensuremath{\mu}L of Canagliflozin spiking solutions into a 15 ml graduated dry centrifuge tube and vortexed for 30 sec. Added 1 ml of Acetonitrile into the above centrifugation tube and mixed. Vortexed the mixture for 3 min. Centrifuged the mixture in the centrifuge tube at 4200 rpm for 12 min. The supernatant liquid after the centrifugation was been filtered using 0.45 \ensuremath{\mu} membrane filter.


\begin{table*}[!htbp]
\caption{\boldmath {Matrix sample long term stability of Canagliflozin at -80\ensuremath{\pm}5 \ensuremath{^\circ}C (60 Days)} }
\label{tw-768ddb598ffd}
\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.21490000000000002\linewidth-2\tabcolsep}p{\dimexpr.20330000000000002\linewidth-2\tabcolsep}p{\dimexpr.20169999999999998\linewidth-2\tabcolsep}p{\dimexpr.18839999999999996\linewidth-2\tabcolsep}p{\dimexpr.1917\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}{\multirow{3}{*}{S No}} & \multicolumn{2}{p{\dimexpr(.405\linewidth-2\tabcolsep)}}{\cAlignHack HQC (1.9 \ensuremath{\mu}g/mL)} & \multicolumn{2}{p{\dimexpr(.3801\linewidth-2\tabcolsep)}}{\cAlignHack LQC (0.18 \ensuremath{\mu}g/mL)}\\
\rowcolor{kwdboxcolor} & \multicolumn{4}{p{\dimexpr(.7850999999999999\linewidth-2\tabcolsep)}}{\cAlignHack Concentration Found (\ensuremath{\mu}g/ml)}\\
\rowcolor{kwdboxcolor} & Control Sample & Stability sample & Control Sample & Stability sample\\
\tblmidrule 
1 &
  \cAlignHack 1.923 &
  \cAlignHack 1.921 &
  \cAlignHack 0.178 &
  \cAlignHack 0.182\\
2 &
  \cAlignHack 1.915 &
  \cAlignHack 1.924 &
  \cAlignHack 0.181 &
  \cAlignHack 0.184\\
3 &
  \cAlignHack 1.918 &
  \cAlignHack 1.917 &
  \cAlignHack 0.176 &
  \cAlignHack 0.185\\
4 &
  \cAlignHack 1.922 &
  \cAlignHack 1.915 &
  \cAlignHack 0.175 &
  \cAlignHack 0.177\\
5 &
  \cAlignHack 1.919 &
  \cAlignHack 1.920 &
  \cAlignHack 0.182 &
  \cAlignHack 0.181\\
6 &
  \cAlignHack 1.917 &
  \cAlignHack 1.922 &
  \cAlignHack 0.184 &
  \cAlignHack 0.178\\
\textbf{Mean} \mbox{}\protect\newline \textbf{Concentration} &
  \cAlignHack 1.919 &
  \cAlignHack 1.919 &
  \cAlignHack 0.1793 &
  \cAlignHack 0.1811\\
\textbf{SD} &
  \cAlignHack 0.0031 &
  \cAlignHack 0.0034 &
  \cAlignHack 0.0036 &
  \cAlignHack 0.0032\\
\textbf{\% CV} &
  \cAlignHack 0.16 &
  \cAlignHack 0.17 &
  \cAlignHack 1.98 &
  \cAlignHack 1.76\\
\textbf{\%Mean accuracy} &
  \cAlignHack 99.95 &
  \cAlignHack 99.99 &
  \cAlignHack 99.63 &
  \cAlignHack 100.65\\
\textbf{Mean stability (\%)} &
  \cAlignHack \textbf{99.97} &
   &
  \cAlignHack \textbf{100.14} &
  \\
\tblbottomrule 
\end{tabulary}\par 
\end{table*}

    
\section{Results and Discussion}
\textbf{Chromatographic conditions development }Method finalization was performed on basis of retention time, USP Tailing factor, USP Resolution and peak areas found for Canagliflozin and that of internal standard. The mobile phase pH was finalized on the basis to shorten the run time (15 min) and different trials were performed during method optimization. Peak retention time of Canagliflozin and Dapagliflozin were found to be about 8.7 min and 4.1 min respectively. 

USP plate count for Dapagliflozin peak and Canagliflozin peak are found to be 3100 and 6500 respectively (from Medium QC (MQC) solution). USP tailing for Dapagliflozin peak and Canagliflozin peak are found to be 1.05 and 1.03 respectively (MQC solution). USP resolution between Dapagliflozin peak and Canagliflozin peak was found to be 13.7 (MQC solution). The reported method is with shorter run time and more number of aliquots can be analyzed in less time. Typical chromatogram of MQC solution obtained by optimized chromatographic condition is presented in Figure~\ref{f-1012b1a51437}.

\textbf{Method Validation (Bio-Analytical) }The developed RP-HPLC method for the estimation of Canagliflozin was validated by following ICH Guidelines. \ensuremath{^{}}

\textbf{System Suitability-Evaluation }Evaluation of System suitability parameters was performed by giving 6 replicate injections of MQC concentration. Evaluation of System suitability was performed on every day sequence as the first experiment. Results of system suitability evaluations are presented in Table~\ref{tw-6aa3b7766b62}.

\textbf{Selectivity/Specificity}

Specificity of the proposed method is validated by analyzing blank samples of the biological matrix (6 batches of di-potassium ethylene diamine tetra acetic acid blank plasma and extracted blank plasma). No peak was observed in the retention time of Canagliflozin (analyte peak) and that of Dapagliflozin (internal standard). A typical chromatogram of plasma extracted blank and chromatogram of internal standard are shown in Figures~\ref{f-f5d1053c2469} and~\ref{f-297be67985e2}. Carryover was studied by injecting the blank sample after the sample (MQC) and no impact of carryover was found.

\textbf{Linearity}

The method linearity was demonstrated over the range of 0.06 to 2.4 \ensuremath{\mu}g /ml for Canagliflozin. The coefficient of correlation (R\ensuremath{^{2}}) value was found to be 0.999 (Limit: not less than 0.99). The relationship adequately describes the response of the instrument with that of concentration of Canagliflozin. The slope and y-intercept of the calibration curve were 0.108 and 0.001 respectively. A specimen calibration curve obtained during the precision and accuracy study of validation is presented in Figure~\ref{f-c4b54c556646}. The back calculated nominal concentrations obtained for calibration standard are presented in Table~\ref{tw-fc641ccd573e} along with the mean calculated accuracy value.

\textbf{Precision and Accuracy}

Within day and between day precision and accuracy were studied by determining 6 replicates of Lower Limit of Quantitation (LLOQC), Low (LQC), MQC and High (HQC). Accuracy and Precision method validation parameter were evaluated by 6 replicate analysis for Canagliflozin at 4 concentration levels, i.e., 0.06 \ensuremath{\mu}g/ml (LLOQC), 0.18 \ensuremath{\mu}g/ml (LQC), 1.2 \ensuremath{\mu}g/ml (MQC) and 1.9 \ensuremath{\mu}g/ml (HQC). Within-day and between day accuracy of plasma samples were studied and excellent mean \% accuracy were obtained with range varied from 97.84 \% to 99.81 \% for within day and 97.22 \% to 100.00 \% for between day respectively. Precision of canagliflozin in plasma samples were studied and found to be 0.23 \% to 5.54 \% for within day and 0.14 \% to 4.01 \% for between days respectively (Tables~\ref{tw-fdcc53ac050a}, \ref{tw-d780c865b618}, \ref{tw-f45183fb4d03}, \ref{tw-43ddb48c533e} and~\ref{tw-038a76a20fb5}).

\textbf{Recovery}

Recovery studies are performed by measuring the peak areas found for Canagliflozin and that of internal standard, by comparing the results of extracted sample with corresponding extracts of blank spiked with Canagliflozin post-extraction. Accuracy values obtained for Canagliflozin at LQC, MQC and HQC were found to be 93.42 \%, 97.96 \% and 92.35\% respectively (Table~\ref{tw-bd3f577b585a}). Overall average accuracy of Dapagliflozin (Internal Standard) was found to be 98.07\%.

\textbf{Solution Stability}

\textbf{Short term stock solution stability}

During bench-top solution stability studies, 6 replicates of LQC \& HQC samples (0.12 and 3.2 \ensuremath{\mu}g/ml) were analyzed after 9 hours at controlled room temperature on the laboratory bench (about 25\ensuremath{^\circ}C). The mean stability of solution was determined and found to be 99.72 \% for LQC and 99.99 \% for HQC respectively. 

\textbf{Long term Matrix sample Solution stability at -28\ensuremath{\pm}5 }\textbf{\ensuremath{^\circ}} \textbf{C \& -80\ensuremath{\pm}5 \ensuremath{^{0}}C for 60 Days}

Long term matrix stock solution stability for the Canagliflozin was studied at a concentration of LQC-HQC level after a storage period of about 60 days at -28\ensuremath{^\circ}C \& -80\ensuremath{^\circ}C in refrigerator. The mean solution stability of Canagliflozin was 99.63 \%, 99.96 \% \& 100.14 \% and 99.97 \% (Tables~\ref{tw-843449f23aa6} and~\ref{tw-768ddb598ffd}).
    
\section{Conclusions}
The novel method validated as per ICH guidelines met with the preset acceptance criterias. This novel bio-analytical method can be used for pharmacokinetic studies in the clinical laboratories.

\textbf{Funding Support}

The authors declare that they have no funding support for this study.

\textbf{Conflict of Interest}

The authors declare that they have no conflict of interest for this study.
    

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