\documentclass[twocolumn,twoside]{article}
\makeatletter\if@twocolumn\PassOptionsToPackage{switch}{lineno}\else\fi\makeatother

\usepackage{amsfonts,amssymb,amsbsy,latexsym,amsmath,tabulary,graphicx,times,xcolor}

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
% Following additional macros are required to function some 
% functions which are not available in the class used.
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
\usepackage{url,multirow,morefloats,floatflt,cancel,tfrupee}
\makeatletter


\AtBeginDocument{\@ifpackageloaded{textcomp}{}{\usepackage{textcomp}}}
\makeatother
\usepackage{colortbl}
\usepackage{xcolor}
\usepackage{pifont}
\usepackage[nointegrals]{wasysym}
\urlstyle{rm}
\makeatletter

%%%For Table column width calculation.
\def\mcWidth#1{\csname TY@F#1\endcsname+\tabcolsep}

%%Hacking center and right align for table
\def\cAlignHack{\rightskip\@flushglue\leftskip\@flushglue\parindent\z@\parfillskip\z@skip}
\def\rAlignHack{\rightskip\z@skip\leftskip\@flushglue \parindent\z@\parfillskip\z@skip}

%Etal definition in references
\@ifundefined{etal}{\def\etal{\textit{et~al}}}{}


%\if@twocolumn\usepackage{dblfloatfix}\fi
\usepackage{ifxetex}
\ifxetex\else\if@twocolumn\@ifpackageloaded{stfloats}{}{\usepackage{dblfloatfix}}\fi\fi

\AtBeginDocument{
\expandafter\ifx\csname eqalign\endcsname\relax
\def\eqalign#1{\null\vcenter{\def\\{\cr}\openup\jot\m@th
  \ialign{\strut$\displaystyle{##}$\hfil&$\displaystyle{{}##}$\hfil
      \crcr#1\crcr}}\,}
\fi
}

%For fixing hardfail when unicode letters appear inside table with endfloat
\AtBeginDocument{%
  \@ifpackageloaded{endfloat}%
   {\renewcommand\efloat@iwrite[1]{\immediate\expandafter\protected@write\csname efloat@post#1\endcsname{}}}{\newif\ifefloat@tables}%
}%

\def\BreakURLText#1{\@tfor\brk@tempa:=#1\do{\brk@tempa\hskip0pt}}
\let\lt=<
\let\gt=>
\def\processVert{\ifmmode|\else\textbar\fi}
\let\processvert\processVert

\@ifundefined{subparagraph}{
\def\subparagraph{\@startsection{paragraph}{5}{2\parindent}{0ex plus 0.1ex minus 0.1ex}%
{0ex}{\normalfont\small\itshape}}%
}{}

% These are now gobbled, so won't appear in the PDF.
\newcommand\role[1]{\unskip}
\newcommand\aucollab[1]{\unskip}
  
\@ifundefined{tsGraphicsScaleX}{\gdef\tsGraphicsScaleX{1}}{}
\@ifundefined{tsGraphicsScaleY}{\gdef\tsGraphicsScaleY{.9}}{}
% To automatically resize figures to fit inside the text area
\def\checkGraphicsWidth{\ifdim\Gin@nat@width>\linewidth
	\tsGraphicsScaleX\linewidth\else\Gin@nat@width\fi}

\def\checkGraphicsHeight{\ifdim\Gin@nat@height>.9\textheight
	\tsGraphicsScaleY\textheight\else\Gin@nat@height\fi}

\def\fixFloatSize#1{}%\@ifundefined{processdelayedfloats}{\setbox0=\hbox{\includegraphics{#1}}\ifnum\wd0<\columnwidth\relax\renewenvironment{figure*}{\begin{figure}}{\end{figure}}\fi}{}}
\let\ts@includegraphics\includegraphics

\def\inlinegraphic[#1]#2{{\edef\@tempa{#1}\edef\baseline@shift{\ifx\@tempa\@empty0\else#1\fi}\edef\tempZ{\the\numexpr(\numexpr(\baseline@shift*\f@size/100))}\protect\raisebox{\tempZ pt}{\ts@includegraphics{#2}}}}

%\renewcommand{\includegraphics}[1]{\ts@includegraphics[width=\checkGraphicsWidth]{#1}}
\AtBeginDocument{\def\includegraphics{\@ifnextchar[{\ts@includegraphics}{\ts@includegraphics[width=\checkGraphicsWidth,height=\checkGraphicsHeight,keepaspectratio]}}}

\DeclareMathAlphabet{\mathpzc}{OT1}{pzc}{m}{it}

\def\URL#1#2{\@ifundefined{href}{#2}{\href{#1}{#2}}}

%%For url break
\def\UrlOrds{\do\*\do\-\do\~\do\'\do\"\do\-}%
\g@addto@macro{\UrlBreaks}{\UrlOrds}



\edef\fntEncoding{\f@encoding}
\def\EUoneEnc{EU1}
\makeatother
\def\floatpagefraction{0.8} 
\def\dblfloatpagefraction{0.8}
\def\style#1#2{#2}
\def\xxxguillemotleft{\fontencoding{T1}\selectfont\guillemotleft}
\def\xxxguillemotright{\fontencoding{T1}\selectfont\guillemotright}

\newif\ifmultipleabstract\multipleabstractfalse%
\newenvironment{typesetAbstractGroup}{}{}%

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%

\usepackage[authoryear]{natbib}



\newif\ifnokeywords\nokeywordsfalse

\makeatletter\input{size10-pointfive.clo}\makeatother%
\definecolor{kwdboxcolor}{RGB}{242,242,242}
\usepackage[hidelinks,colorlinks=true,allcolors=blue]{hyperref}
\linespread{1}                 
\def\floatpagefraction{0.8}

\usepackage[paperheight=11.69in,paperwidth=8.26in,top=1in,bottom=1in,left=1in,right=.75in,headsep=24pt]{geometry}

\usepackage{multirow-custom}

\makeatletter

\def\hlinewd#1{%
  \noalign{\ifnum0=`}\fi\hrule \@height #1%
  \futurelet\reserved@a\@xhline}
\def\tbltoprule{\hlinewd{1pt}\\[-14pt]}
\def\tblbottomrule{\noalign{\vspace*{6pt}}\hline\noalign{\vspace*{2pt}}}
\def\tblmidrule{\hline\noalign{\vspace*{2pt}}}



\let\@articleType\@empty
\let\@journalDoi\@empty
\let\@journalVolume\@empty
\let\@journalIssue\@empty
\let\@crossMarkLink\@empty
\let\@receivedDate\@empty
\let\@acceptedDate\@empty
\let\@revisedDate\@empty
\let\@publishedDate\@empty
\let\@copyrightYear\@empty
\let\@firstPage\@empty
\def\articleType#1{\gdef\@articleType{#1}}
\def\journalDoi#1{\gdef\@journalDoi{#1}}
\def\crossMarkLink#1{\gdef\@crossMarkLink{#1}}
\def\receivedDate#1{\gdef\@receivedDate{#1}}
\def\acceptedDate#1{\gdef\@acceptedDate{#1}}
\def\revisedDate#1{\gdef\@revisedDate{#1}}
\def\publishedDate#1{\gdef\@publishedDate{#1}}
\def\copyrightYear#1{\gdef\@copyrightYear{#1}}
\def\journalVolume#1{\gdef\@journalVolume{#1}}
\def\journalIssue#1{\gdef\@journalIssue{#1}}
\def\firstPage#1{\gdef\@firstPage{#1}}
\let\@issuelogo\@empty%
\let\@issuelogoex\@empty%
\def\issuelogo#1{\gdef\@issuelogo{#1}}
\def\issuelogoex#1{\gdef\@issuelogoex{#1}}


\def\author#1{%
  \gdef\@author{%
    \hskip-\dimexpr(\tabcolsep)\hskip5pt%
    \parbox{\dimexpr\textwidth-1pt}%
    {\fontsize{11}{13}\selectfont\raggedright #1}%
  }%
}


\usepackage{pharmascope-abs}
\usepackage{caption}
\usepackage{lastpage}
\usepackage{fancyhdr}
\usepackage[noindentafter,explicit]{titlesec}
\usepackage{fontspec}

\setmainfont[%
BoldFont=cambriab.ttf,%
ItalicFont=CAMBRIAI.TTF,%
BoldItalicFont=CAMBRIAZ.TTF]{Cambria.ttf}
\lefthyphenmin = 3

\def\title#1{%
  \gdef\@title{%
      \vspace*{-40pt}%
       \ifx\@articleType\@empty\else{\fontsize{10}{12}\scshape\selectfont\hspace{8pt}\@articleType\hfill\mbox{}\par\vspace{2pt}}\fi%
       \minipage{\linewidth}
         \hrulefill\\[-0.7pt]%%{\includegraphics[width=75pt,height=50pt]{ijrps_logo.png}}\hfill
         \mbox{~}\hspace{5pt}\parbox{.1\linewidth}{\IfFileExists{images/\@issuelogo.\@issuelogoex}{\includegraphics[width=79pt,height=61pt]{images/\@issuelogo.\@issuelogoex}}{\includegraphics[width=75pt,height=50pt]{ijrps_logo.png}}}\hfill
         \fcolorbox{kwdboxcolor}{kwdboxcolor}{\parbox{.792\linewidth}{%
          \begin{center}\fontsize{17}{17}\selectfont\scshape\vskip-7pt International Journal of Research in Pharmaceutical Sciences\hfill\end{center}%
          \vspace*{-10pt}\hspace*{4pt}{\fontsize{8}{9}\selectfont Published by JK Welfare \& Pharmascope Foundation\hfill  Journal Home Page: \href{www.ijrps.com}{\color{blue}\underline{\smash{www.ijrps.com}}}}\hspace*{4pt}\mbox{}}}%
       \par\vspace*{-1pt}\rule{\linewidth}{1.3pt}%
       \endminipage%
    \par\vspace*{9.2pt}\parbox{.98\linewidth}{\linespread{.9}\raggedright\fontsize{14}{17}\selectfont #1}%
    \vspace*{-8pt}%
  }
}


\setlength{\parindent}{0pt}
\setlength{\parskip}{0.4pc plus 1pt minus 1pt}

\def\abbrvJournalTitle{Int. J. Res. Pharm. Sci.}

\fancypagestyle{headings}{%
  \renewcommand{\headrulewidth}{0pt}%
  \renewcommand{\footrulewidth}{0.3pt}
  \fancyhf{}%
  \fancyhead[R]{%
    \fontsize{9.12}{11}\selectfont\RunningAuthor,\ \abbrvJournalTitle,\ \ifx\@journalVolume\@empty X\else\@journalVolume\fi%
    \ifx\@journalIssue\@empty\else(\@journalIssue)\fi%
    ,\ \ifx\@firstPage\@empty 1\else\@firstPage\fi-\pageref*{LastPage}%
  }%
  \fancyfoot[LO,RE]{\fontsize{9.12}{11}\selectfont\textcopyright\ International Journal of Research in Pharmaceutical Sciences}%
  \fancyfoot[RO,LE]{\fontsize{9.12}{11}\selectfont\thepage}
}\pagestyle{headings}

                                                         
\fancypagestyle{plain}{%
  \renewcommand{\headrulewidth}{0pt}%
  \renewcommand{\footrulewidth}{0.3pt}%
  \fancyhf{}%
  \fancyhead[R]{%
    \fontsize{9.12}{11}\selectfont\RunningAuthor,\ \abbrvJournalTitle,\ \ifx\@journalVolume\@empty X\else\@journalVolume\fi%
    \ifx\@journalIssue\@empty\else(\@journalIssue)\fi%
    ,\ \ifx\@firstPage\@empty 1\else\@firstPage\fi-\pageref*{LastPage}%
  }%
  \fancyfoot[LO,RE]{\fontsize{9.12}{11}\selectfont\textcopyright\ International Journal of Research in Pharmaceutical Sciences}%
  \fancyfoot[RO,LE]{\fontsize{9.12}{11}\selectfont\thepage}
  \ifx\@firstPage\@empty\else\setcounter{page}{\@firstPage}\fi
}


\def\NormalBaseline{\def\baselinestretch{1.1}}
\usepackage{textcase}
\setcounter{secnumdepth}{0}

\titleformat{\section}[block]{\bfseries\boldmath\NormalBaseline\filright\fontsize{10.5}{13}\selectfont}
{\thesection}
{6pt}
{\MakeTextUppercase{#1}}
[]
\titleformat{\subsection}[block]{\bfseries\boldmath\NormalBaseline\filright\fontsize{10.5}{12}\selectfont}
{\thesubsection}
{6pt}
{#1}
[]
\titleformat{\subsubsection}[block]{\NormalBaseline\filright\fontsize{10.5}{12}\selectfont}
{\thesubsubsection}
{6pt}
{#1}
[]
\titleformat{\paragraph}[block]{\NormalBaseline\filright\fontsize{10.5}{10}\selectfont}
{\theparagraph}
{6pt}
{#1}
[]
\titleformat{\subparagraph}[block]{\NormalBaseline\filright\fontsize{10.5}{12}\selectfont}
{\thesubparagraph}
{6pt}
{#1}
[]

\titlespacing{\section}{0pt}{.5\baselineskip}{.5\baselineskip}  
\titlespacing{\subsection}{0pt}{.5\baselineskip}{.5\baselineskip}  
\titlespacing{\subsubsection}{0pt}{.5\baselineskip}{.5\baselineskip}  
\titlespacing{\paragraph}{0pt}{.5\baselineskip}{.5\baselineskip}  
\titlespacing{\subparagraph}{0pt}{.5\baselineskip}{.5\baselineskip}

\captionsetup[figure]{skip=1.4pt,font=bf,labelsep=colon,justification=raggedright,singlelinecheck=false}
\captionsetup[table]{skip=1.4pt,font=bf,labelsep=colon,justification=raggedright,singlelinecheck=false}

\def\bibyear#1{#1}
%\def\bibjtitle#1{#1}   %%Publisher request
\def\bibauand{}
\setlength\bibsep{3pt}
\setlength\bibhang{8pt}

\makeatother
\date{}


\begin{document}


\def\RunningAuthor{Yasodai R et al.}
\firstPage{2090}
\articleType{Original Article}
\receivedDate{10 Jan 2020}
\acceptedDate{15 Feb 2020}
\revisedDate{12 Feb 2020}
\journalVolume{2020, 11}
\journalIssue{2}
\journalDoi{ijrps.v11i2.2150}
\copyrightYear{2020}
\def\authorCount{3}
\def\affCount{3}

\def\journalTitle{International Journal of Research in Pharmaceutical Sciences}

\title{Phytochemical analysis and quantitative nutritional evaluvation of zingiber officinale roscae (Ginger)}
\author{Yasodai~R\textsuperscript{*}\textsuperscript{1},  
            Kavimani~M\textsuperscript{2}, 
            Prabhu~K\textsuperscript{3}~\\[5pt]{\textsuperscript{1}Research scholar, Department of Anatomy, Bharat University, Seliyur, Chennai, Tamilnadu, India}~\\{\textsuperscript{2}Department of anatomy, Sree Balaji medical college \& hospital, Chennai, Tamilnadu, India}~\\{\textsuperscript{3}Sree Balaji medical college \& hospital, Chennai, Tamilnadu, India}}

\begin{abstract}
Evaluate the phytochemical analysis and nutritional value of the zingiber officinale roscae and its therapeutic utility. Phytochemical analysis is to determine the value of bioactive compounds and minerals in the plants. Phytochemical screening results showed that the presence of alkaloids, saponins, flavonoids, polyphenols, tannins like compound in the various extraction methods. The quantitative study was done by using gas chromatography method and mass spectroscopy analysis. Then the screening of all the analysis revealed that the plant has good metabolites which justify that it has therapeutic utility. The result of the proximate analysis is revealed that in zingiber officinale roscae. It is rich in calcium (46\%) when compared to other elements like sodium, iron, cadmium, manganese, mercury which are present in the standard deviation value 1.007 to 0.005\%. the most bioactive compound like gingerol, shagoal, curcumin and other compounds were shown in the qualitative analysis. The rhizome of zingiber officinale is also known as ginger. It is the most popular herb used in various foods and beverages and also traditional medicines. The herb is used to various diseases like stomach disorders, diabetes, hyper tension, cancer, spasm, etc .The chemical constituents which are present in the ginger like gingerol, shagoal, etc. The ginger has the properties like anticancer, antitumor, anti inflammatory, neuroprotective, hepto-protective activity etc.
\end{abstract}\def\keywordstitle{Keywords}

\begin{keywords}Zingiber officinale roscae -ginger,\newline Gas chromatography -GC,\newline Mass spectroscopy analysis-MS,\newline Phytochemical  screening-PS
\end{keywords}
\twocolumn[ \maketitle {\printKwdAbsBox}]
\makeatletter{\raggedright\textsuperscript{*}Corresponding Author\par Name:\ Yasodai~R~\\ Phone:\ 9698750695~\\  Email:\ yasoanatomy@gmail.com
        \par}\vspace*{-11pt}\hrulefill\par{\fontsize{12}{14}\selectfont ISSN: 0975-7538}\par% 
        \textsc{DOI:}\ \href{https://doi.org/10.26452/\@journalDoi}{\textcolor{blue}{\underline{\smash{https://doi.org/10.26452/\@journalDoi}}}}\par%
        \vspace*{-11pt}\hrulefill\\{\fontsize{9.12}{10.12}\selectfont Production and Hosted by}\par{IJRPS |~\href{www.ijrps.com}{www.ijrps.com}}\par%
        \vspace*{-2pt}{\fontsize{9.12}{10.12}\selectfont\textcopyright\ \@copyrightYear\ $|$ All rights reserved.}\par%
        \vspace*{-11pt}\rule{\linewidth}{1.2pt}
        \makeatother
        
    
\section{Introduction}
Zingiber officinale roscae (ginger) is a well-known tropical and sub-tropical shrub medical plant. It belongs to the \textit{Family: zingerberaca} \textit{\space } which contains about 1200 species in 50 genera belongs to monocotyledon family. It is semi-woody perennial rhizome plant about 3-4 mm higher. It grows rapidly in all the parts and of used for flavor and as therapeutic agents. This plant contains a wide variety of biologically active compounds, non-active compounds called phytochemicals, moreover it contains 400 compounds \unskip~\citep{705065:16715131,705065:16715134} which include alkaloids, saponins, steroids, tannins, glycosides, amnioacids, proteins, vitamins which is widely used and has the properties like anticancer, antitumor, anti-inflammatory, neuroprotective , hepto-protective activity, antiemetic, analgesic, hypotensive, antioxidant etc. \unskip~\citep{705065:16715127}. This plant is being used from ancient times to treat various disorders like diabetes, snakebites, stomach disorder, toothaches respiratory disorders, bleedings and skin burns.
    
\section{Materials and Methods}
\textbf{Collection \& preparations of the samples}

The rhizome of zingiber officinale (ginger) were collected from a farm near Coimbatore, Tamilnadu, India. The collection of the sample was done by plucking the plants from the soil and placed into a plastic bag. The collected samples were washed and then dried, powdered and stored.

\textbf{The Apparatus and reagent}

The analysis of Na, Manganese, Fe, Cr, Cd, Lead, N, Hg (Table~\ref{tw-d69a1e85aec1}) were done by atomic absorption spectrophotometer. 

To quantify the level of Sodium was done by flame photometer using standard Enlrich volumetric concentrated metallic ion calibration method. All the chemical reagents were grade checked and double distilled water were used.

Q\textbf{uantitative and Qualitative analysis} -\textbf{Gas chromatography technique and mass spectroscopy analysis}

It consists of gc-2010 gas chromatography. The components were separated on Rtx-MS quartz capillary (60M x0.26M) with the cross band 95\%. Dimethyl poly silicone at stationary phase 80 degree for 1min. 0.3m\ensuremath{\mu} volume of sample injections with spilt ratio 1: 20 for 30mins.

This analysis is used for quantitative and qualitative compositions of the extract \unskip~\citep{705065:16715133,705065:16715129}.


\begin{table}[!htbp]
\caption{\boldmath {Phytochemical analysis} }
\label{tw-d69a1e85aec1}
\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.5572\linewidth-2\tabcolsep}p{\dimexpr.4428\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}\cAlignHack Phytochemical screening & \cAlignHack  Values\\
\tblmidrule 
Mineral elements &
  Compositions\\
Calcium  &
  46.6 \ensuremath{\pm}1.16\\
Sodium &
  31.2\ensuremath{\pm}0.15\\
Iron &
  25.5\ensuremath{\pm}0.6\\
Copper &
  25.5\ensuremath{\pm}0.3\\
Zinc &
  21\ensuremath{\pm}0.11\\
Manganese &
  15\ensuremath{\pm}.5\\
Chromium &
  3.70\ensuremath{\pm}0.008\\
Cadmium &
  0.070\ensuremath{\pm}0.03\\
Lead &
  1.050\ensuremath{\pm}0.02\\
Nickel &
  0.070\ensuremath{\pm}0.01\\
Mercury &
  0.20\ensuremath{\pm}0.01\\
\tblbottomrule 
\end{tabulary}\par 
\end{table}

\begin{table}[!htbp]
\caption{\boldmath {Quantitative analysis of 100\% (petroluem etherextract)} }
\label{tw-ea989a9d17eb}
\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.7132\linewidth-2\tabcolsep}p{\dimexpr.2868\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}Compounds & Areas \%\\
\tblmidrule 
Gingiberene (15.4\%) &
  (15.4)\\
Beta-seiquphelendene  &
  (18.1) \\
Curcumen  &
  (11.2\%)\\
Glycohexanonal &
  (0.6)\%\\
Gingerol &
  (4.46\%)\\
Shagoal  &
  (7.45\%)\\
Cadinene &
  ( 1.2\%)\\
\tblbottomrule 
\end{tabulary}\par 
\end{table}
\textbf{Phytochemical screening}

To analyze the presence of alkaloids, saponins, tannins, steroids, glycosides, terpenoids, phlobotannins were carried out by method harbored.

\textbf{Alkaloid}

5g of ginger extract with 5 ml of pure honey was mixed with 5ml of 1\% aqueous HCL stream 1ml few drops of dragged off reagent. The presence of blue-black color turbidity indicates that the presence of alkaloids.

\textbf{Saponin}

5grams of ginger extract with 5 ml honey was shaken with distilled water it results frothing, presence of saponins.

\textbf{Tannins}

5grams of ginger extract with 5-6ml honey with distilled water and filtered. Tannins results Blue black color appearance.


\begin{table*}[!htbp]
\caption{\boldmath {Composition of zingiber officinale rhizome (mg/100Dm) \ensuremath{\pm} standard deviation} }
\label{tw-40f7f926a260}
\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.2483\linewidth-2\tabcolsep}p{\dimexpr.2743\linewidth-2\tabcolsep}p{\dimexpr.2413\linewidth-2\tabcolsep}p{\dimexpr.2361\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}\cAlignHack Bioactive & \cAlignHack Methanol extract & \cAlignHack Ethanol extract & \cAlignHack Petroleum ether extract\\
\tblmidrule 
Alkaloids &
  \cAlignHack  +++ &
  \cAlignHack  +++ &
  \cAlignHack  -\\
Tannins &
  \cAlignHack  ++ &
  \cAlignHack  - &
  \cAlignHack  -\\
Glycosides &
  \cAlignHack  ++ &
  \cAlignHack  + &
  \cAlignHack  +\\
Saponins &
  \cAlignHack  +++ &
  \cAlignHack  +++ &
  \cAlignHack  -\\
Steroids &
  \cAlignHack  -- &
  \cAlignHack  - &
  \cAlignHack  +\\
Flavonoids &
  \cAlignHack  ++ &
  \cAlignHack  ++ &
  \cAlignHack  ++\\
Terpenoids &
  \cAlignHack  + &
  \cAlignHack  - &
  \cAlignHack  -\\
Phlobotannins &
  \cAlignHack  + &
  \cAlignHack  + &
  \cAlignHack  ++\\
Polyphenols &
  \cAlignHack  ++ &
  \cAlignHack  + &
  \cAlignHack  +\\
Anthraquinone &
  \cAlignHack  - &
  \cAlignHack  - &
  \cAlignHack  -\\
Anthranoids &
  \cAlignHack  -- &
  \cAlignHack  - &
  \cAlignHack  -\\
\tblbottomrule 
\end{tabulary}\par 
\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {4-Quantitative analysis: Ethanolic extract (100\%)} }
\label{tw-7d07a10113dc}
\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.15290000000000003\linewidth-2\tabcolsep}p{\dimexpr.21709999999999997\linewidth-2\tabcolsep}p{\dimexpr.2292\linewidth-2\tabcolsep}p{\dimexpr.4008\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}No. & RT & Area (\%) & Compounds\\
\tblmidrule 
1. &
  6.3 &
  0.061 &
  Cineole\\
2. &
  8.3 &
  0.682 &
  Campol\\
3. &
  11.3 &
  0.233 &
  Cyclo-isosativene\\
4. &
  11.7 &
  0.484 &
  Tricyclo \\
5. &
  12.4 &
  0.354 &
  Beta- Farnesene\\
6. &
  12.7 &
  0.276 &
  2,6,10Dodecatrien-1-ol\\
7. &
  12.7 &
  0.847 &
  Spiro [4,5] dec\\
8. &
  13.6 &
  10.278 &
   Curcumin\\
9. &
  13.8 &
  19.579 &
   Gingiberene\\
10. &
  12.6 &
  8.770 &
   Fernesene\\
11. &
  13.5 &
  9.611 &
  Cyclo Hexane\\
12. &
  13.4 &
  2.412 &
  Cadinene\\
13. &
  13.7 &
  11.713 &
  \ensuremath{\beta } Seiquphellandrene\\
14. &
  14.5 &
  0.144 &
  \ensuremath{\alpha } Pano-sinsen\\
15. &
  15.4 &
  0.055 &
  B-Nerolidol\\
16. &
  22.6 &
  6.456 &
  Cis-6-Shagole\\
17. &
  23.2 &
  3.467 &
  Gingerol\\
18 &
  24.1 &
  0.968 &
  Gingerol\\
19. &
  24.6 &
  0.639 &
  Capsaicin\\
20. &
  25.8 &
  0.840 &
  Trans-10-Shagole\\
21 &
  18.3 &
  0.651 &
  \ensuremath{\delta } Tocopherol\\
\tblbottomrule 
\end{tabulary}\par 
\end{table*}

\begin{table*}[!htbp]
\caption{\boldmath {Quantitative analysis : Methanolic extract (100\%)} }
\label{tw-343a70620441}
\def\arraystretch{1.1}
\ignorespaces 
\centering 
\begin{tabulary}{\linewidth}{p{\dimexpr.1461\linewidth-2\tabcolsep}p{\dimexpr.1859\linewidth-2\tabcolsep}p{\dimexpr.19970000000000002\linewidth-2\tabcolsep}p{\dimexpr.4683\linewidth-2\tabcolsep}}
\tbltoprule \rowcolor{kwdboxcolor}No. & RT & Area (\%) & Compounds\\
\tblmidrule 
1. &
  12.43 &
  0.121 &
   \ensuremath{\beta } Farnesene\\
2. &
  12.8 &
  0.281 &
  Spiro [4,5] \\
3. &
  13.0 &
  7.311 &
   Curcumen\\
4. &
  13.2 &
  14.432 &
   Gingiberene\\
5. &
  13.9 &
  75.22 &
  Fernesene\\
6. &
  13.5 &
  7.629 &
  2,6,10Dodecatrien-1-ol\\
7. &
  13.5 &
  1.713 &
  \ensuremath{\gamma } {\textendash}cadinene\\
8. &
  13.8 &
  10.88 &
  \ensuremath{\beta } Sei-quphellandrene\\
9. &
  14.4 &
  0.92 &
  B Nerolidol\\
10. &
  15.8 &
  0.90 &
  Zingiberen\\
11. &
  15.9 &
  0.199 &
  Guaol\\
12. &
  15.9 &
  0.264 &
  Di-methyl-3,8 Nonadien-2-one\\
13. &
  16.1 &
  0.376 &
  qisabinene Hydrate\\
14. &
  16.3 &
  0.448 &
  Rosifaliol\\
15. &
  16.4 &
  0.554 &
  \ensuremath{\beta } {\textendash} Bisabolol\\
16. &
  16.8 &
  0.620 &
  3Farnesol\\
17. &
  17.5 &
  0.729 &
  Gemacron\\
18. &
  18.3 &
  0.870 &
  2-Norbornanone\\
19. &
  18.4 &
  0.983 &
  Thiiofenchone\\
20. &
  18.6 &
  0.031 &
  Veridiflorol\\
21. &
  19.1 &
  0.139 &
  Dlepi \ensuremath{\alpha }Cedrenepoxide\\
22. &
  19.4 &
  0.233 &
  Methyl Icosanoate\\
23. &
  19.7 &
  0.324 &
  Verbenol 3 Caren\\
24. &
  19.9 &
  0.432 &
  Curcumene\\
25. &
  20.1 &
  0.595 &
  Carveol\\
26. &
  20.7 &
  0.615 &
  \ensuremath{\beta } - pinen, 3(acetylmethyl)\\
27. &
  21.0 &
  0.746 &
  Methyl linoleate\\
28. &
  21.3 &
  0.850 &
  2,5 dibutyl-furan\\
29. &
  21.6 &
  0.942 &
  Decalin,\\
30. &
  22.0 &
  0.020 &
  Nerol propionate\\
31. &
  22.6 &
  4.172 &
   6 shagaol\\
32. &
  23.0 &
  12.278 &
  Gingerol\\
33. &
  23.6 &
  0.320 &
  2- Formy thexadecane\\
34. &
  24.1 &
  0.475 &
  Lariciresinol\\
35. &
  24.4 &
  1.536 &
  Gingerol\\
\tblbottomrule 
\end{tabulary}\par 
\end{table*}
\textbf{Phlobotannins}

Observation of red precipitate, determine that phlobo-tannins is present.

\textbf{Flavonoids}

5 ml of dilute of ammonium solution were added with filt. Con. H\ensuremath{_{2\ }}SO\ensuremath{_{4\ }}inside of the test tube. Yellow colorations -determine that the presence of flavonoids.

\textbf{Steroids}

2 ml of acidic hydride with 0.5grams of extracts with 5ml of con.H\ensuremath{_{2}}SO\ensuremath{_{4\ ,\ }} were added inside of the test tube. The presence of violet coloration results steroid.

\textbf{Terpenods}

0.6 grams of ginger extract with 2ml of chloroform was added with con.H\ensuremath{_{2}}SO\ensuremath{_{4\ }} results brown coloration.
    
\section{Results and Discussion}
The Phytochemical analysis of zingiber officinalis shows with peak value of the chemical composition of the extract by CG-MS facilitate to identify the bioactive compounds \unskip~\citep{705065:16715136}. The major substance which are present in the ethanolic extract (Table~\ref{tw-ea989a9d17eb}, Table~\ref{tw-7d07a10113dc}, Table~\ref{tw-343a70620441}), \unskip~\citep{705065:16715135} were Gingiberene (20.4\%), Beta- seiquphelendene (12.1\%), Curcumin (11.2\%), Glycohexanonal (0.6\%), Gingerol (4.46\%), Shagoal (7.45\%) and etc. The methanolic petroleum ether extract were Gingiberene (15.4\%), Beta- seiquphelendene (18.1), Curcumen (11.2\%), Glycohexanonal (0.6\%), Gingerol (4.46\%), Shagoal (7.45\%), Cadinene (1.2\%) etc.\unskip~\citep{705065:16715136} are present.

\textbf{Phytochemical screening}

To analyze the presence of alkaloids, saponins, tannins, glycosides, terpenoids, phlobotannins were present (Table~\ref{tw-40f7f926a260}) in the methanolic and ethanolic and petroleum ether extract \unskip~\citep{705065:16715128} in the absence of steroids \unskip~\citep{705065:16715129}.

The glycoside plays the vital role to the support its strength and the rate of contraction, alkaloids have more physiological effects on the humans is more used in the relief of pain, saponions (Table~\ref{tw-ea989a9d17eb}) shows the strong expectorant and it help in the absorption of nutrient. Flavoids were possess more antioxidant properties to strengthen the capillary walls. Overall, the phytochemical and bioactive compounds of the zingiber officinalis \unskip~\citep{705065:16715132} shows many properties like anticancer, anti-tumor, anti-inflammatory, neuroprotective, hepto-protective activity, antifungal, antibacterial, Gastro-protective activity \unskip~\citep{705065:16715130}.

Spectrophotometer is to analysis the mineral elements (Table~\ref{tw-d69a1e85aec1}) which are zingiber officinalis like iron, sodium, zinc, calcium, Mn, Cr, Cd,Hg and N. Zingiber officinalis contain less amount of iron(26.6\%g), low amount of mercury and zinc. The amount of sodium which present in zingiberofficinalis 31.2\% and calcium [1] obtained more amount 47.60\%, its plays important role for blood coagulation and also for the intracellular component.
    
\section{Conclusion }
Zingiber officinalis extract solvents of various forms have more pharmacological values, because it contains alkaloids, saponins, tannins, glycosides, terpenoids, phlobatannin and other essential compounds. So it is considered to be a potential source of medicinal herbs. We can extend research on its properties.
    

\bibliographystyle{pharmascope_apa-custom}

\bibliography{\jobname}
\end{document}
