Synthesis, Characterization, Antimicrobial, DNA Cleavage and Fluorescent Activity of Metal ion(II) Coordinate with 2H-Chromene Azo novel ligand

Abstract

A new ligand 2H-chromene containing azo group 2-(4-nitrophenyl)-N-(4-(phenyldiazenyl)-2H-chromen-4-amine (AH), were synthesized from the condensation reaction (1:2) of 2'-hydroxychalcone and p-aminoazobenzene. Co(II), Cu(II) and Ni(II) complexes of the new ligand have been synthesized and characterized using C.H.N. analysis, 1H NMR spectra, FT.IR, UV/Visible, magnetic susceptible, conductance measuring, and fluorescence spectral spectroscopy; 13C NMR spectroscopy of the ligand was also studied. Spectroscopic results revealed the 2H-Chromene Azo (AH) ligand behaves as monodentate chelating via the nitrogen atom of amine group at position 4 having 1:1 [M:L] ratio; suggested that the cobalt and nickel complexes have the tetrahedral structure and a distorted tetrahedral geometry for the copper complex, indicating their non-electrolyte nature. The new ligand shows a fluorescence emissioncomparing with this fluorescence quenching was noticed in its metal complexes. The antibacterial potency of the free ligand and its chelates with metal ion(II) were screened against E.coli, K.pneumoniae, Staph.aureus  and B.Subtilis; The DNA cleavage activity of the free ligand and its 2H-chromene azo metal (II) complexes was performed by the gel electrophoresis process, which given that these compounds are effective upon DNA cleavage.